Histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (Bos grunniens)
To adapt to the extreme conditions of plateau environments, yaks have evolved thick hair, making them an ideal model for investigating the mechanisms involved in hair growth. We can gain valuable insights into how hair follicles develop and their cyclic growth in challenging environments by studying...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1277586/full |
| _version_ | 1797596383913443328 |
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| author | Bo Liao Yan Cui Yan Cui Sijiu Yu Junfeng He Xue Yang Shengnan Zou Sijie Li Pengfei Zhao Hongwei Xu Min Long Xiaoyan Wang |
| author_facet | Bo Liao Yan Cui Yan Cui Sijiu Yu Junfeng He Xue Yang Shengnan Zou Sijie Li Pengfei Zhao Hongwei Xu Min Long Xiaoyan Wang |
| author_sort | Bo Liao |
| collection | DOAJ |
| description | To adapt to the extreme conditions of plateau environments, yaks have evolved thick hair, making them an ideal model for investigating the mechanisms involved in hair growth. We can gain valuable insights into how hair follicles develop and their cyclic growth in challenging environments by studying yaks. However, the lack of essential data on yak hair follicle histology and the absence of in vitro cell models for hair follicles serve as a limitation to such research objectives. In this study, we investigated the structure of skin tissue during different hair follicle cycles using the yak model. Additionally, we successfully established in vitro models of hair follicle-associated cells derived from yak skin, including dermal papilla cells (DPCs), preadipocytes, and fibroblasts. We optimized the microdissection technique for DPCs culture by simplifying the procedure and reducing the time required. Furthermore, we improved the methodology used to differentiate yak preadipocytes into mature adipocytes, thus increasing the differentiation efficiency. The introduction of yak as a natural model provides valuable research resources for exploring the mechanisms of hair growth and contributes to a deeper understanding of hair follicle biology and the development of regenerative medicine strategies. |
| first_indexed | 2024-03-11T02:49:40Z |
| format | Article |
| id | doaj.art-5deba2ba084a4e65a71235640fefceec |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-11T02:49:40Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-5deba2ba084a4e65a71235640fefceec2023-11-18T09:03:00ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-11-011010.3389/fvets.2023.12775861277586Histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (Bos grunniens)Bo Liao0Yan Cui1Yan Cui2Sijiu Yu3Junfeng He4Xue Yang5Shengnan Zou6Sijie Li7Pengfei Zhao8Hongwei Xu9Min Long10Xiaoyan Wang11College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou, ChinaGansu Province Livestock Embryo Engineering Research Center, Lanzhou, ChinaGansu Province Livestock Embryo Engineering Research Center, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou, ChinaTo adapt to the extreme conditions of plateau environments, yaks have evolved thick hair, making them an ideal model for investigating the mechanisms involved in hair growth. We can gain valuable insights into how hair follicles develop and their cyclic growth in challenging environments by studying yaks. However, the lack of essential data on yak hair follicle histology and the absence of in vitro cell models for hair follicles serve as a limitation to such research objectives. In this study, we investigated the structure of skin tissue during different hair follicle cycles using the yak model. Additionally, we successfully established in vitro models of hair follicle-associated cells derived from yak skin, including dermal papilla cells (DPCs), preadipocytes, and fibroblasts. We optimized the microdissection technique for DPCs culture by simplifying the procedure and reducing the time required. Furthermore, we improved the methodology used to differentiate yak preadipocytes into mature adipocytes, thus increasing the differentiation efficiency. The introduction of yak as a natural model provides valuable research resources for exploring the mechanisms of hair growth and contributes to a deeper understanding of hair follicle biology and the development of regenerative medicine strategies.https://www.frontiersin.org/articles/10.3389/fvets.2023.1277586/fullyakhair cyclefibroblastspreadipocytesinduced differentiationdermal papilla cells |
| spellingShingle | Bo Liao Yan Cui Yan Cui Sijiu Yu Junfeng He Xue Yang Shengnan Zou Sijie Li Pengfei Zhao Hongwei Xu Min Long Xiaoyan Wang Histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (Bos grunniens) Frontiers in Veterinary Science yak hair cycle fibroblasts preadipocytes induced differentiation dermal papilla cells |
| title | Histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (Bos grunniens) |
| title_full | Histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (Bos grunniens) |
| title_fullStr | Histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (Bos grunniens) |
| title_full_unstemmed | Histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (Bos grunniens) |
| title_short | Histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (Bos grunniens) |
| title_sort | histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle associated cells of yak bos grunniens |
| topic | yak hair cycle fibroblasts preadipocytes induced differentiation dermal papilla cells |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1277586/full |
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