<i>MC1R</i> Is a Prognostic Marker and Its Expression Is Correlated with MSI in Colorectal Cancer
Melanocortin 1 receptor (<i>MC1R</i>) is thought to be a marker of poor prognosis and a potential target for the treatment of melanoma. Studies have found that <i>MC1R</i> promotes several tumor behaviors, including cell proliferation and differentiation, pigment formation, a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
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| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/43/3/108 |
| Summary: | Melanocortin 1 receptor (<i>MC1R</i>) is thought to be a marker of poor prognosis and a potential target for the treatment of melanoma. Studies have found that <i>MC1R</i> promotes several tumor behaviors, including cell proliferation and differentiation, pigment formation, and genome damage repair. Some single-nucleotide polymorphisms (SNPs) of <i>MC1R</i> are involved in the occurrence and development of melanoma. A few studies have reported a relationship between <i>MC1R</i> and colorectal cancer (CRC). In this research, our objective was to examine <i>MC1R</i> expression and <i>MC1R</i> SNPs and investigate their correlation with the clinicopathological features of human CRC tissues. We evaluated <i>MC1R</i> mRNA expression by performing bioinformatic analyses on human CRC expression datasets. We used Western blotting and RT-qPCR to compare <i>MC1R</i> expression in CRC tissues with that in normal tissues, and <i>MC1R</i> SNPs in CRC tissues were detected by PCR-direct sequencing (DS). The expression of <i>MC1R</i> was significantly decreased in CRC tissues compared with normal tissue, and its expression was negatively associated with <i>P53</i> expression, <i>MLH1</i> expression, and <i>PMS2</i> expression, and high <i>MC1R</i> expression was significantly associated with microsatellite instability (MSI). <i>MC1R</i> SNPs were also associated with the clinicopathological characteristics of CRC; for example, the rs2228479 locus genotype was correlated with <i>Ki67</i> status, and the rs885479 locus genotype was correlated with age and T stage. In conclusion, <i>MC1R</i> plays a crucial role in the progression of CRC and may be a marker of poor prognosis in CRC. |
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| ISSN: | 1467-3037 1467-3045 |