<i>MC1R</i> Is a Prognostic Marker and Its Expression Is Correlated with MSI in Colorectal Cancer
Melanocortin 1 receptor (<i>MC1R</i>) is thought to be a marker of poor prognosis and a potential target for the treatment of melanoma. Studies have found that <i>MC1R</i> promotes several tumor behaviors, including cell proliferation and differentiation, pigment formation, a...
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MDPI AG
2021-10-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/43/3/108 |
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| author | Lixiong Peng Jiang Chang Xilin Liu Shiying Lu Honglin Ren Xiaoshi Zhou Zengshan Liu Pan Hu |
| author_facet | Lixiong Peng Jiang Chang Xilin Liu Shiying Lu Honglin Ren Xiaoshi Zhou Zengshan Liu Pan Hu |
| author_sort | Lixiong Peng |
| collection | DOAJ |
| description | Melanocortin 1 receptor (<i>MC1R</i>) is thought to be a marker of poor prognosis and a potential target for the treatment of melanoma. Studies have found that <i>MC1R</i> promotes several tumor behaviors, including cell proliferation and differentiation, pigment formation, and genome damage repair. Some single-nucleotide polymorphisms (SNPs) of <i>MC1R</i> are involved in the occurrence and development of melanoma. A few studies have reported a relationship between <i>MC1R</i> and colorectal cancer (CRC). In this research, our objective was to examine <i>MC1R</i> expression and <i>MC1R</i> SNPs and investigate their correlation with the clinicopathological features of human CRC tissues. We evaluated <i>MC1R</i> mRNA expression by performing bioinformatic analyses on human CRC expression datasets. We used Western blotting and RT-qPCR to compare <i>MC1R</i> expression in CRC tissues with that in normal tissues, and <i>MC1R</i> SNPs in CRC tissues were detected by PCR-direct sequencing (DS). The expression of <i>MC1R</i> was significantly decreased in CRC tissues compared with normal tissue, and its expression was negatively associated with <i>P53</i> expression, <i>MLH1</i> expression, and <i>PMS2</i> expression, and high <i>MC1R</i> expression was significantly associated with microsatellite instability (MSI). <i>MC1R</i> SNPs were also associated with the clinicopathological characteristics of CRC; for example, the rs2228479 locus genotype was correlated with <i>Ki67</i> status, and the rs885479 locus genotype was correlated with age and T stage. In conclusion, <i>MC1R</i> plays a crucial role in the progression of CRC and may be a marker of poor prognosis in CRC. |
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| language | English |
| last_indexed | 2024-03-10T04:23:52Z |
| publishDate | 2021-10-01 |
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| series | Current Issues in Molecular Biology |
| spelling | doaj.art-5debc70fff924a17a1f731795ab2577c2023-11-23T07:43:45ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452021-10-014331529154710.3390/cimb43030108<i>MC1R</i> Is a Prognostic Marker and Its Expression Is Correlated with MSI in Colorectal CancerLixiong Peng0Jiang Chang1Xilin Liu2Shiying Lu3Honglin Ren4Xiaoshi Zhou5Zengshan Liu6Pan Hu7Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Double-First Class Discipline of Human-Animal Medicine, Jilin University, Changchun 130015, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Double-First Class Discipline of Human-Animal Medicine, Jilin University, Changchun 130015, ChinaNorman Bethune Health Science Center, Jilin University, Changchun 130015, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Double-First Class Discipline of Human-Animal Medicine, Jilin University, Changchun 130015, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Double-First Class Discipline of Human-Animal Medicine, Jilin University, Changchun 130015, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Double-First Class Discipline of Human-Animal Medicine, Jilin University, Changchun 130015, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Double-First Class Discipline of Human-Animal Medicine, Jilin University, Changchun 130015, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Double-First Class Discipline of Human-Animal Medicine, Jilin University, Changchun 130015, ChinaMelanocortin 1 receptor (<i>MC1R</i>) is thought to be a marker of poor prognosis and a potential target for the treatment of melanoma. Studies have found that <i>MC1R</i> promotes several tumor behaviors, including cell proliferation and differentiation, pigment formation, and genome damage repair. Some single-nucleotide polymorphisms (SNPs) of <i>MC1R</i> are involved in the occurrence and development of melanoma. A few studies have reported a relationship between <i>MC1R</i> and colorectal cancer (CRC). In this research, our objective was to examine <i>MC1R</i> expression and <i>MC1R</i> SNPs and investigate their correlation with the clinicopathological features of human CRC tissues. We evaluated <i>MC1R</i> mRNA expression by performing bioinformatic analyses on human CRC expression datasets. We used Western blotting and RT-qPCR to compare <i>MC1R</i> expression in CRC tissues with that in normal tissues, and <i>MC1R</i> SNPs in CRC tissues were detected by PCR-direct sequencing (DS). The expression of <i>MC1R</i> was significantly decreased in CRC tissues compared with normal tissue, and its expression was negatively associated with <i>P53</i> expression, <i>MLH1</i> expression, and <i>PMS2</i> expression, and high <i>MC1R</i> expression was significantly associated with microsatellite instability (MSI). <i>MC1R</i> SNPs were also associated with the clinicopathological characteristics of CRC; for example, the rs2228479 locus genotype was correlated with <i>Ki67</i> status, and the rs885479 locus genotype was correlated with age and T stage. In conclusion, <i>MC1R</i> plays a crucial role in the progression of CRC and may be a marker of poor prognosis in CRC.https://www.mdpi.com/1467-3045/43/3/108<i>MC1R</i>CRCSNPsclinicopathologic featuresprognostic marker |
| spellingShingle | Lixiong Peng Jiang Chang Xilin Liu Shiying Lu Honglin Ren Xiaoshi Zhou Zengshan Liu Pan Hu <i>MC1R</i> Is a Prognostic Marker and Its Expression Is Correlated with MSI in Colorectal Cancer Current Issues in Molecular Biology <i>MC1R</i> CRC SNPs clinicopathologic features prognostic marker |
| title | <i>MC1R</i> Is a Prognostic Marker and Its Expression Is Correlated with MSI in Colorectal Cancer |
| title_full | <i>MC1R</i> Is a Prognostic Marker and Its Expression Is Correlated with MSI in Colorectal Cancer |
| title_fullStr | <i>MC1R</i> Is a Prognostic Marker and Its Expression Is Correlated with MSI in Colorectal Cancer |
| title_full_unstemmed | <i>MC1R</i> Is a Prognostic Marker and Its Expression Is Correlated with MSI in Colorectal Cancer |
| title_short | <i>MC1R</i> Is a Prognostic Marker and Its Expression Is Correlated with MSI in Colorectal Cancer |
| title_sort | i mc1r i is a prognostic marker and its expression is correlated with msi in colorectal cancer |
| topic | <i>MC1R</i> CRC SNPs clinicopathologic features prognostic marker |
| url | https://www.mdpi.com/1467-3045/43/3/108 |
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