Genotypic Distribution and the Epidemiology of Multidrug Resistant Tuberculosis in Upper Northern Thailand
The epidemiology and genotypes of multidrug-resistant tuberculosis (MDR-TB), a global public health threat, remain limited. The genotypic distribution and factors associated with MDR-TB in upper northern Thailand between 2015 and 2019 were investigated. The DNA sequencing of <i>rpoB</i>,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/12/1733 |
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| author | Sukanya Saikaew Aksara Thongprachum Rodjana Pongsararuk Aungkana Thanraka Naowarat Kunyanone Boonchai Chaiyasirinroje Praphan Luangsook Bordin Butr-Indr Ponrut Phunpae Usanee Wattananandkul |
| author_facet | Sukanya Saikaew Aksara Thongprachum Rodjana Pongsararuk Aungkana Thanraka Naowarat Kunyanone Boonchai Chaiyasirinroje Praphan Luangsook Bordin Butr-Indr Ponrut Phunpae Usanee Wattananandkul |
| author_sort | Sukanya Saikaew |
| collection | DOAJ |
| description | The epidemiology and genotypes of multidrug-resistant tuberculosis (MDR-TB), a global public health threat, remain limited. The genotypic distribution and factors associated with MDR-TB in upper northern Thailand between 2015 and 2019 were investigated. The DNA sequencing of <i>rpoB</i>, <i>katG</i>, and <i>inhA</i> promoter of 51 multidrug-resistant <i>Mycobacterium tuberculosis</i> isolates revealed nine patterns of the <i>rpoB</i> gene mutation distributed in seven provinces. The S531L mutation was the most common mutation in all provinces. The <i>rpoB</i> mutation in Chiang Rai, Chiang Mai, and Lampang was highly diverse compared to other areas. Here, the mutation profiles that have yet to be reported in northern Thailand (H526P, Q513P, and H526C) were detected in Chiang Rai province. The S315T <i>katG</i> mutation was the most common genotype associated with INH resistance, especially in Chiang Mai and Lampang. Further analysis of data from 110 TB patients (42 MDR-TB and 68 drug-susceptible TB) revealed that <60 years of age was a significant factor associated with MDR-TB (OR = 0.316, 95% CI 0.128–0.784, <i>p</i> = 0.011) and ≥60 years of age was a significant factor associated with the S315T <i>katG</i>-mutation (OR = 8.867, 95% CI 0.981–80.177, <i>p</i> = 0.047). This study highlighted the necessity for continuous surveillance and risk factor monitoring for effective control of MDR-TB. |
| first_indexed | 2024-03-09T17:23:58Z |
| format | Article |
| id | doaj.art-5dedb64277fa40ef918e8958cece039f |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T17:23:58Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-5dedb64277fa40ef918e8958cece039f2023-11-24T12:53:25ZengMDPI AGAntibiotics2079-63822022-12-011112173310.3390/antibiotics11121733Genotypic Distribution and the Epidemiology of Multidrug Resistant Tuberculosis in Upper Northern ThailandSukanya Saikaew0Aksara Thongprachum1Rodjana Pongsararuk2Aungkana Thanraka3Naowarat Kunyanone4Boonchai Chaiyasirinroje5Praphan Luangsook6Bordin Butr-Indr7Ponrut Phunpae8Usanee Wattananandkul9Faculty of Public Health, Chiang Mai University, Muang District, Chiang Mai 50200, ThailandFaculty of Public Health, Chiang Mai University, Muang District, Chiang Mai 50200, ThailandOffice of Disease Prevention and Control, 1 (ODPC 1) Chiang Mai, Department of Disease Control, Ministry of Public Health Thailand, Chiang Mai 50000, ThailandDepartment of Medical Technology, Chiangrai Prachanukroh Hospital, Chiang Rai 57000, ThailandDepartment of Medical Technology, Chiangrai Prachanukroh Hospital, Chiang Rai 57000, ThailandTB/HIV Research Foundation (THRF), Chiang Rai 57000, ThailandDivision of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, ThailandDivision of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, ThailandDivision of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, ThailandDivision of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, ThailandThe epidemiology and genotypes of multidrug-resistant tuberculosis (MDR-TB), a global public health threat, remain limited. The genotypic distribution and factors associated with MDR-TB in upper northern Thailand between 2015 and 2019 were investigated. The DNA sequencing of <i>rpoB</i>, <i>katG</i>, and <i>inhA</i> promoter of 51 multidrug-resistant <i>Mycobacterium tuberculosis</i> isolates revealed nine patterns of the <i>rpoB</i> gene mutation distributed in seven provinces. The S531L mutation was the most common mutation in all provinces. The <i>rpoB</i> mutation in Chiang Rai, Chiang Mai, and Lampang was highly diverse compared to other areas. Here, the mutation profiles that have yet to be reported in northern Thailand (H526P, Q513P, and H526C) were detected in Chiang Rai province. The S315T <i>katG</i> mutation was the most common genotype associated with INH resistance, especially in Chiang Mai and Lampang. Further analysis of data from 110 TB patients (42 MDR-TB and 68 drug-susceptible TB) revealed that <60 years of age was a significant factor associated with MDR-TB (OR = 0.316, 95% CI 0.128–0.784, <i>p</i> = 0.011) and ≥60 years of age was a significant factor associated with the S315T <i>katG</i>-mutation (OR = 8.867, 95% CI 0.981–80.177, <i>p</i> = 0.047). This study highlighted the necessity for continuous surveillance and risk factor monitoring for effective control of MDR-TB.https://www.mdpi.com/2079-6382/11/12/1733rifampicin resistanceisoniazid resistancemutationtuberculosisrisk factor |
| spellingShingle | Sukanya Saikaew Aksara Thongprachum Rodjana Pongsararuk Aungkana Thanraka Naowarat Kunyanone Boonchai Chaiyasirinroje Praphan Luangsook Bordin Butr-Indr Ponrut Phunpae Usanee Wattananandkul Genotypic Distribution and the Epidemiology of Multidrug Resistant Tuberculosis in Upper Northern Thailand Antibiotics rifampicin resistance isoniazid resistance mutation tuberculosis risk factor |
| title | Genotypic Distribution and the Epidemiology of Multidrug Resistant Tuberculosis in Upper Northern Thailand |
| title_full | Genotypic Distribution and the Epidemiology of Multidrug Resistant Tuberculosis in Upper Northern Thailand |
| title_fullStr | Genotypic Distribution and the Epidemiology of Multidrug Resistant Tuberculosis in Upper Northern Thailand |
| title_full_unstemmed | Genotypic Distribution and the Epidemiology of Multidrug Resistant Tuberculosis in Upper Northern Thailand |
| title_short | Genotypic Distribution and the Epidemiology of Multidrug Resistant Tuberculosis in Upper Northern Thailand |
| title_sort | genotypic distribution and the epidemiology of multidrug resistant tuberculosis in upper northern thailand |
| topic | rifampicin resistance isoniazid resistance mutation tuberculosis risk factor |
| url | https://www.mdpi.com/2079-6382/11/12/1733 |
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