Mathematical model of astaxanthin purification process using the low-pressure column chromatography method
Astaxanthin is an antioxidant compound that can be extracted from the microalgae Haematococcus pluvialis using the microwave-assisted extraction (MAE) method. Purification of the astaxanthin compound can be conducted using a low-pressure column chromatography (LPCC) method. However, the mathematical...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | South African Journal of Chemical Engineering |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1026918523000501 |
| _version_ | 1797776650978459648 |
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| author | Putri Restu Dewati Rochmadi Abdul Rohman Arief Budiman |
| author_facet | Putri Restu Dewati Rochmadi Abdul Rohman Arief Budiman |
| author_sort | Putri Restu Dewati |
| collection | DOAJ |
| description | Astaxanthin is an antioxidant compound that can be extracted from the microalgae Haematococcus pluvialis using the microwave-assisted extraction (MAE) method. Purification of the astaxanthin compound can be conducted using a low-pressure column chromatography (LPCC) method. However, the mathematical model illustrating the adsorption and desorption process using the LPCC method has not been built by the other authors yet. Therefore, the purposes of this study were (1) to build the mathematical model of the purification process using the LPCC method, and (2) to simulate the experimental data using the model to find the important kinetic constant values that can be used in the industrial scale-up in the future. The purification process was carried out with silica gel as a stationary phase and a mixture of n-hexane:acetone of 3:1 (v/v) as a mobile phase. The silica gel diameter was varied to 0.2–0.5 mm and 0.063–0.2 mm, while the eluent velocity was varied to 1.85 mL/min and 3 mL/min. The mathematical model having five kinetic constants of De1, De2, kc2, kc2.a, and H_A2 was successfully built. The De1 and De2 (m2/s) were the effective diffusivities of astaxanthin in the intraparticle direction of the adsorbent granule and in the axial direction of the column, respectively. The kc2 (m/s) was the mass transfer coefficient of astaxanthin from the solution to the adsorbent. The kc2.a (/s) was the volumetric mass transfer coefficient of astaxanthin from the solution to the adsorbent. The H_A2 (g silica gel/m3) was the Henry's constant for astaxanthin concentration at equilibrium at the liquid-solid interface. In the variation of silica gel diameter, the De2 value at the diameter of 0.2–0.5 mm was greater than that at 0.063–0.2 mm. However, the De1, kc2, kc2.a, and H_A2 values were not affected by the different diameters. In the variation of eluent velocity, the kc2 and kc2.a values at 3 mL/min were greater than those at 1.85 mL/min. However, the De1, De2, and H_A2 values were not affected by the different eluent velocities. |
| first_indexed | 2024-03-12T22:53:57Z |
| format | Article |
| id | doaj.art-5deec61800fa48b0adff1f2de9464b96 |
| institution | Directory Open Access Journal |
| issn | 1026-9185 |
| language | English |
| last_indexed | 2024-03-12T22:53:57Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | South African Journal of Chemical Engineering |
| spelling | doaj.art-5deec61800fa48b0adff1f2de9464b962023-07-20T04:37:43ZengElsevierSouth African Journal of Chemical Engineering1026-91852023-07-0145256268Mathematical model of astaxanthin purification process using the low-pressure column chromatography methodPutri Restu Dewati0 Rochmadi1Abdul Rohman2Arief Budiman3Chemical Engineering Department, Universitas Pembangunan Nasional Veteran Yogyakarta, Jl. SWK104, Yogyakarta 55283, IndonesiaChemical Engineering Department, Universitas Gadjah Mada, Jl. Grafika 2, Yogyakarta 55284, IndonesiaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, 55281, IndonesiaChemical Engineering Department, Universitas Gadjah Mada, Jl. Grafika 2, Yogyakarta 55284, Indonesia; Center of Excellence for Microalgae Biorefinery, Universitas Gadjah Mada, Sekip K1A, Yogyakarta, 55281, Indonesia; Corresponding author.Astaxanthin is an antioxidant compound that can be extracted from the microalgae Haematococcus pluvialis using the microwave-assisted extraction (MAE) method. Purification of the astaxanthin compound can be conducted using a low-pressure column chromatography (LPCC) method. However, the mathematical model illustrating the adsorption and desorption process using the LPCC method has not been built by the other authors yet. Therefore, the purposes of this study were (1) to build the mathematical model of the purification process using the LPCC method, and (2) to simulate the experimental data using the model to find the important kinetic constant values that can be used in the industrial scale-up in the future. The purification process was carried out with silica gel as a stationary phase and a mixture of n-hexane:acetone of 3:1 (v/v) as a mobile phase. The silica gel diameter was varied to 0.2–0.5 mm and 0.063–0.2 mm, while the eluent velocity was varied to 1.85 mL/min and 3 mL/min. The mathematical model having five kinetic constants of De1, De2, kc2, kc2.a, and H_A2 was successfully built. The De1 and De2 (m2/s) were the effective diffusivities of astaxanthin in the intraparticle direction of the adsorbent granule and in the axial direction of the column, respectively. The kc2 (m/s) was the mass transfer coefficient of astaxanthin from the solution to the adsorbent. The kc2.a (/s) was the volumetric mass transfer coefficient of astaxanthin from the solution to the adsorbent. The H_A2 (g silica gel/m3) was the Henry's constant for astaxanthin concentration at equilibrium at the liquid-solid interface. In the variation of silica gel diameter, the De2 value at the diameter of 0.2–0.5 mm was greater than that at 0.063–0.2 mm. However, the De1, kc2, kc2.a, and H_A2 values were not affected by the different diameters. In the variation of eluent velocity, the kc2 and kc2.a values at 3 mL/min were greater than those at 1.85 mL/min. However, the De1, De2, and H_A2 values were not affected by the different eluent velocities.http://www.sciencedirect.com/science/article/pii/S1026918523000501AstaxanthinMathematical modelLow-pressure column chromatographyPurification |
| spellingShingle | Putri Restu Dewati Rochmadi Abdul Rohman Arief Budiman Mathematical model of astaxanthin purification process using the low-pressure column chromatography method South African Journal of Chemical Engineering Astaxanthin Mathematical model Low-pressure column chromatography Purification |
| title | Mathematical model of astaxanthin purification process using the low-pressure column chromatography method |
| title_full | Mathematical model of astaxanthin purification process using the low-pressure column chromatography method |
| title_fullStr | Mathematical model of astaxanthin purification process using the low-pressure column chromatography method |
| title_full_unstemmed | Mathematical model of astaxanthin purification process using the low-pressure column chromatography method |
| title_short | Mathematical model of astaxanthin purification process using the low-pressure column chromatography method |
| title_sort | mathematical model of astaxanthin purification process using the low pressure column chromatography method |
| topic | Astaxanthin Mathematical model Low-pressure column chromatography Purification |
| url | http://www.sciencedirect.com/science/article/pii/S1026918523000501 |
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