Level of Foxp3, DNMTs, methylation of Foxp3 promoter region, and CD4 + CD25 + CD127low regulatory T cells in vulvar lichen sclerosus
Abstract This study is to investigate the pathogenesis of vulvar lichen sclerosus (VLS) by analyzing the level of Foxp3, DNMTs, methylation of Foxp3 promoter region, and CD4 + CD25 + CD127low Regulatory T cells (Tregs). This study enrolled 15 VLS patients and 25 controls. Lesional and extralesional...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-06-01
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| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/kjm2.12356 |
| _version_ | 1818865265526439936 |
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| author | Lin Wang Jin‐Ling Yi Hai‐Yan Chen Pei‐Liang Wang Yan‐Li Shen |
| author_facet | Lin Wang Jin‐Ling Yi Hai‐Yan Chen Pei‐Liang Wang Yan‐Li Shen |
| author_sort | Lin Wang |
| collection | DOAJ |
| description | Abstract This study is to investigate the pathogenesis of vulvar lichen sclerosus (VLS) by analyzing the level of Foxp3, DNMTs, methylation of Foxp3 promoter region, and CD4 + CD25 + CD127low Regulatory T cells (Tregs). This study enrolled 15 VLS patients and 25 controls. Lesional and extralesional vulvar skin tissues, normal vulvar skin tissues and peripheral blood were collected. Compared with the control group, Foxp3 protein in the lesional and extralesional skin of VLS group was significantly reduced. The levels of DNMT1 and DNMT3b proteins in lesional skin of VLS group were significantly increased. There was no difference in the total methylation rates of the promoter region of the Foxp3 gene. The methylation rates of CpG1, CpG4, CpG9, and CpG10 were significantly higher in lesional skin of VLS group than in control group. There was no correlation between the total methylation rates of 10 CpG sites and the level of Foxp3 and DNMT1 proteins; there was a positive correlation between Foxp3 and DNMT1 protein in lesional skin of VLS group (r = 0.675, p < 0.05), and a negative correlation (r = −0.665, p < 0.05) in extralesional skin of VLS group. However, there was no correlation of Foxp3 with DNMT3b. The number of CD4 + CD25 + CD127low Tregs VLS decreased significantly. The expression of Foxp3 protein and the quantity of CD4 + CD25 + CD127low Tregs in patients with VLS decreased, which may cause local or systemic abnormal immunosuppression of Tregs, leading to the occurrence of VLS. This may be related with methylation or DNMT1, which needs further verification. |
| first_indexed | 2024-12-19T10:44:48Z |
| format | Article |
| id | doaj.art-5df0ad69efb14bbdba9df05978102381 |
| institution | Directory Open Access Journal |
| issn | 1607-551X 2410-8650 |
| language | English |
| last_indexed | 2024-12-19T10:44:48Z |
| publishDate | 2021-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-5df0ad69efb14bbdba9df059781023812022-12-21T20:25:19ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502021-06-0137652052710.1002/kjm2.12356Level of Foxp3, DNMTs, methylation of Foxp3 promoter region, and CD4 + CD25 + CD127low regulatory T cells in vulvar lichen sclerosusLin WangJin‐Ling Yi0Hai‐Yan Chen1Pei‐Liang Wang2Yan‐Li Shen3Department of Gynecology the Fifth Affiliated Hospital of Xinjiang Medical University Urumqi Xinjiang ChinaDepartment of Gynecology the Fifth Affiliated Hospital of Xinjiang Medical University Urumqi Xinjiang ChinaDepartment of Gynecology the Fifth Affiliated Hospital of Xinjiang Medical University Urumqi Xinjiang ChinaDepartment of Gynecology the Fifth Affiliated Hospital of Xinjiang Medical University Urumqi Xinjiang ChinaAbstract This study is to investigate the pathogenesis of vulvar lichen sclerosus (VLS) by analyzing the level of Foxp3, DNMTs, methylation of Foxp3 promoter region, and CD4 + CD25 + CD127low Regulatory T cells (Tregs). This study enrolled 15 VLS patients and 25 controls. Lesional and extralesional vulvar skin tissues, normal vulvar skin tissues and peripheral blood were collected. Compared with the control group, Foxp3 protein in the lesional and extralesional skin of VLS group was significantly reduced. The levels of DNMT1 and DNMT3b proteins in lesional skin of VLS group were significantly increased. There was no difference in the total methylation rates of the promoter region of the Foxp3 gene. The methylation rates of CpG1, CpG4, CpG9, and CpG10 were significantly higher in lesional skin of VLS group than in control group. There was no correlation between the total methylation rates of 10 CpG sites and the level of Foxp3 and DNMT1 proteins; there was a positive correlation between Foxp3 and DNMT1 protein in lesional skin of VLS group (r = 0.675, p < 0.05), and a negative correlation (r = −0.665, p < 0.05) in extralesional skin of VLS group. However, there was no correlation of Foxp3 with DNMT3b. The number of CD4 + CD25 + CD127low Tregs VLS decreased significantly. The expression of Foxp3 protein and the quantity of CD4 + CD25 + CD127low Tregs in patients with VLS decreased, which may cause local or systemic abnormal immunosuppression of Tregs, leading to the occurrence of VLS. This may be related with methylation or DNMT1, which needs further verification.https://doi.org/10.1002/kjm2.12356CD127CD4 + CD25+ TregFoxp3methylationvulvar lichen sclerosus |
| spellingShingle | Lin Wang Jin‐Ling Yi Hai‐Yan Chen Pei‐Liang Wang Yan‐Li Shen Level of Foxp3, DNMTs, methylation of Foxp3 promoter region, and CD4 + CD25 + CD127low regulatory T cells in vulvar lichen sclerosus Kaohsiung Journal of Medical Sciences CD127 CD4 + CD25+ Treg Foxp3 methylation vulvar lichen sclerosus |
| title | Level of Foxp3, DNMTs, methylation of Foxp3 promoter region, and CD4 + CD25 + CD127low regulatory T cells in vulvar lichen sclerosus |
| title_full | Level of Foxp3, DNMTs, methylation of Foxp3 promoter region, and CD4 + CD25 + CD127low regulatory T cells in vulvar lichen sclerosus |
| title_fullStr | Level of Foxp3, DNMTs, methylation of Foxp3 promoter region, and CD4 + CD25 + CD127low regulatory T cells in vulvar lichen sclerosus |
| title_full_unstemmed | Level of Foxp3, DNMTs, methylation of Foxp3 promoter region, and CD4 + CD25 + CD127low regulatory T cells in vulvar lichen sclerosus |
| title_short | Level of Foxp3, DNMTs, methylation of Foxp3 promoter region, and CD4 + CD25 + CD127low regulatory T cells in vulvar lichen sclerosus |
| title_sort | level of foxp3 dnmts methylation of foxp3 promoter region and cd4 cd25 cd127low regulatory t cells in vulvar lichen sclerosus |
| topic | CD127 CD4 + CD25+ Treg Foxp3 methylation vulvar lichen sclerosus |
| url | https://doi.org/10.1002/kjm2.12356 |
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