ONCOBREAST-TEST Is a Quick Diagnostic, Prognostic and Predictive Method of Response to Systemic Treatment
ONCOBREAST-TEST is a diagnostic and therapeutic procedure that is part of the comprehensive care of a patient with breast cancer.: Chemosensitivity of cancer cells was assessed using the MTT test, morphological assessment of cells, LDH activity in the culture medium, and flow cytometry technique (ap...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/16/1/120 |
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| author | Anna Tankiewicz-Kwedlo Tomasz Lobacz Leszek Kozlowski Bogumila Czartoryska-Arlukowicz Mariusz Koda Krystyna Pawlak Robert Czarnomysy Magdalena Joanna Borkowska Dariusz Pawlak |
| author_facet | Anna Tankiewicz-Kwedlo Tomasz Lobacz Leszek Kozlowski Bogumila Czartoryska-Arlukowicz Mariusz Koda Krystyna Pawlak Robert Czarnomysy Magdalena Joanna Borkowska Dariusz Pawlak |
| author_sort | Anna Tankiewicz-Kwedlo |
| collection | DOAJ |
| description | ONCOBREAST-TEST is a diagnostic and therapeutic procedure that is part of the comprehensive care of a patient with breast cancer.: Chemosensitivity of cancer cells was assessed using the MTT test, morphological assessment of cells, LDH activity in the culture medium, and flow cytometry technique (apoptosis, proliferation, CD24, CD44, GATA3, cytokeratin, Ki-67). Diagnostic tools included panels of simple tests which could be used to accurately predict the chemosensitivity of tumor cells previously isolated from a patient, even before actual chemotherapy. The proposed procedure allows for a simple (based on MTT results, cell morphology, LDH concentration), minimally invasive, quick, and accurate assessment of the sensitivity of breast cancer cells to the drugs used and, to select the most effective treatment plan as part of personalized therapy. In a patient with NOS G3, the most promising therapy will be docetaxel with cyclophosphamide and in the case of a patient with NOS G1, paclitaxel alone and in combination with trastuzumab. The implementation of such a procedure would undoubtedly increase the effectiveness of chemotherapy, reduce side effects by excluding drugs that are ineffective before using them, protect the patient’s health, and shorten the treatment time, bringing economic and social benefits. |
| first_indexed | 2024-03-08T15:10:22Z |
| format | Article |
| id | doaj.art-5df244f909924fbe97585d5067375845 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-08T15:10:22Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-5df244f909924fbe97585d50673758452024-01-10T14:52:45ZengMDPI AGCancers2072-66942023-12-0116112010.3390/cancers16010120ONCOBREAST-TEST Is a Quick Diagnostic, Prognostic and Predictive Method of Response to Systemic TreatmentAnna Tankiewicz-Kwedlo0Tomasz Lobacz1Leszek Kozlowski2Bogumila Czartoryska-Arlukowicz3Mariusz Koda4Krystyna Pawlak5Robert Czarnomysy6Magdalena Joanna Borkowska7Dariusz Pawlak8Department of Pharmacodynamics, Medical University of Bialystok, Mickiewicza 2C, 15-222 Bialystok, PolandM. Skłodowska-Curie Bialystok Oncology Center, Ogrodowa 12, 15-027 Bialystok, PolandM. Skłodowska-Curie Bialystok Oncology Center, Ogrodowa 12, 15-027 Bialystok, PolandM. Skłodowska-Curie Bialystok Oncology Center, Ogrodowa 12, 15-027 Bialystok, PolandDepartment of General Pathomorphology, Medical University of Bialystok, ul. Waszyngtona 13, 15-269 Bialystok, PolandDepartment of Monitored Pharmacotherapy, Medical University of Bialystok, Mickiewicza 2C, 15-222 Bialystok, PolandDepartment of Synthesis and Technology of Drugs, Medical University of Bialystok, Kilinskiego 1, 15-089 Bialystok, PolandM. Skłodowska-Curie Bialystok Oncology Center, Ogrodowa 12, 15-027 Bialystok, PolandDepartment of Pharmacodynamics, Medical University of Bialystok, Mickiewicza 2C, 15-222 Bialystok, PolandONCOBREAST-TEST is a diagnostic and therapeutic procedure that is part of the comprehensive care of a patient with breast cancer.: Chemosensitivity of cancer cells was assessed using the MTT test, morphological assessment of cells, LDH activity in the culture medium, and flow cytometry technique (apoptosis, proliferation, CD24, CD44, GATA3, cytokeratin, Ki-67). Diagnostic tools included panels of simple tests which could be used to accurately predict the chemosensitivity of tumor cells previously isolated from a patient, even before actual chemotherapy. The proposed procedure allows for a simple (based on MTT results, cell morphology, LDH concentration), minimally invasive, quick, and accurate assessment of the sensitivity of breast cancer cells to the drugs used and, to select the most effective treatment plan as part of personalized therapy. In a patient with NOS G3, the most promising therapy will be docetaxel with cyclophosphamide and in the case of a patient with NOS G1, paclitaxel alone and in combination with trastuzumab. The implementation of such a procedure would undoubtedly increase the effectiveness of chemotherapy, reduce side effects by excluding drugs that are ineffective before using them, protect the patient’s health, and shorten the treatment time, bringing economic and social benefits.https://www.mdpi.com/2072-6694/16/1/120breast cancerchemotherapychemosensitivitypersonalized therapy |
| spellingShingle | Anna Tankiewicz-Kwedlo Tomasz Lobacz Leszek Kozlowski Bogumila Czartoryska-Arlukowicz Mariusz Koda Krystyna Pawlak Robert Czarnomysy Magdalena Joanna Borkowska Dariusz Pawlak ONCOBREAST-TEST Is a Quick Diagnostic, Prognostic and Predictive Method of Response to Systemic Treatment Cancers breast cancer chemotherapy chemosensitivity personalized therapy |
| title | ONCOBREAST-TEST Is a Quick Diagnostic, Prognostic and Predictive Method of Response to Systemic Treatment |
| title_full | ONCOBREAST-TEST Is a Quick Diagnostic, Prognostic and Predictive Method of Response to Systemic Treatment |
| title_fullStr | ONCOBREAST-TEST Is a Quick Diagnostic, Prognostic and Predictive Method of Response to Systemic Treatment |
| title_full_unstemmed | ONCOBREAST-TEST Is a Quick Diagnostic, Prognostic and Predictive Method of Response to Systemic Treatment |
| title_short | ONCOBREAST-TEST Is a Quick Diagnostic, Prognostic and Predictive Method of Response to Systemic Treatment |
| title_sort | oncobreast test is a quick diagnostic prognostic and predictive method of response to systemic treatment |
| topic | breast cancer chemotherapy chemosensitivity personalized therapy |
| url | https://www.mdpi.com/2072-6694/16/1/120 |
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