STUDYING THE EFFECTS OF SOME COMPOUNDS AND IONS ON Escherichia coli ALKALINE PHOSPHATASE ACTIVITY
The effect of some compounds and ions such as EDTA,Urea,KCN,2-Mercaptoethanol,BeCl2 and L-Phenyl alanine on the activity of Alkaline phosphatase extracted from Escherichia coli has been studied by using p-NitroPhenyl Phosphate as a substrate at a different concentrations (1.5„e10-1,3.0„e10-2,4.5„e10...
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| Format: | Article |
| Language: | English |
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University of Anbar
2012-06-01
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| Series: | مجلة جامعة الانبار للعلوم الصرفة |
| Subjects: | |
| Online Access: | https://juaps.uoanbar.edu.iq/article_15558_42ea88f6efdb78a12b11b3c0c15e2f5e.pdf |
| _version_ | 1797373393759109120 |
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| author | KHALID .F.AL-RAWI |
| author_facet | KHALID .F.AL-RAWI |
| author_sort | KHALID .F.AL-RAWI |
| collection | DOAJ |
| description | The effect of some compounds and ions such as EDTA,Urea,KCN,2-Mercaptoethanol,BeCl2 and L-Phenyl alanine on the activity of Alkaline phosphatase extracted from Escherichia coli has been studied by using p-NitroPhenyl Phosphate as a substrate at a different concentrations (1.5„e10-1,3.0„e10-2,4.5„e10-3) M.,and the alkaline glycine regulating solution pH=10.5.Inhibition constant Ki and type of inhibition(competitive or noncompetitive)using Dixon plot for competitive & noncompetitive inhibition as well as the percentage of inhibition have all been calculated & I50 for different compounds. |
| first_indexed | 2024-03-08T18:49:57Z |
| format | Article |
| id | doaj.art-5df2c144a59c4dc8baee8b3318cd93e1 |
| institution | Directory Open Access Journal |
| issn | 1991-8941 2706-6703 |
| language | English |
| last_indexed | 2024-03-08T18:49:57Z |
| publishDate | 2012-06-01 |
| publisher | University of Anbar |
| record_format | Article |
| series | مجلة جامعة الانبار للعلوم الصرفة |
| spelling | doaj.art-5df2c144a59c4dc8baee8b3318cd93e12023-12-28T21:52:09ZengUniversity of Anbarمجلة جامعة الانبار للعلوم الصرفة1991-89412706-67032012-06-0113586910.37652/juaps.2007.1555815558STUDYING THE EFFECTS OF SOME COMPOUNDS AND IONS ON Escherichia coli ALKALINE PHOSPHATASE ACTIVITYKHALID .F.AL-RAWI0Department of Chemistry - College of Education - University of AnbarThe effect of some compounds and ions such as EDTA,Urea,KCN,2-Mercaptoethanol,BeCl2 and L-Phenyl alanine on the activity of Alkaline phosphatase extracted from Escherichia coli has been studied by using p-NitroPhenyl Phosphate as a substrate at a different concentrations (1.5„e10-1,3.0„e10-2,4.5„e10-3) M.,and the alkaline glycine regulating solution pH=10.5.Inhibition constant Ki and type of inhibition(competitive or noncompetitive)using Dixon plot for competitive & noncompetitive inhibition as well as the percentage of inhibition have all been calculated & I50 for different compounds.https://juaps.uoanbar.edu.iq/article_15558_42ea88f6efdb78a12b11b3c0c15e2f5e.pdfcompoundsionsescherichia colialkaline phosphatase |
| spellingShingle | KHALID .F.AL-RAWI STUDYING THE EFFECTS OF SOME COMPOUNDS AND IONS ON Escherichia coli ALKALINE PHOSPHATASE ACTIVITY مجلة جامعة الانبار للعلوم الصرفة compounds ions escherichia coli alkaline phosphatase |
| title | STUDYING THE EFFECTS OF SOME COMPOUNDS AND IONS ON Escherichia coli ALKALINE PHOSPHATASE ACTIVITY |
| title_full | STUDYING THE EFFECTS OF SOME COMPOUNDS AND IONS ON Escherichia coli ALKALINE PHOSPHATASE ACTIVITY |
| title_fullStr | STUDYING THE EFFECTS OF SOME COMPOUNDS AND IONS ON Escherichia coli ALKALINE PHOSPHATASE ACTIVITY |
| title_full_unstemmed | STUDYING THE EFFECTS OF SOME COMPOUNDS AND IONS ON Escherichia coli ALKALINE PHOSPHATASE ACTIVITY |
| title_short | STUDYING THE EFFECTS OF SOME COMPOUNDS AND IONS ON Escherichia coli ALKALINE PHOSPHATASE ACTIVITY |
| title_sort | studying the effects of some compounds and ions on escherichia coli alkaline phosphatase activity |
| topic | compounds ions escherichia coli alkaline phosphatase |
| url | https://juaps.uoanbar.edu.iq/article_15558_42ea88f6efdb78a12b11b3c0c15e2f5e.pdf |
| work_keys_str_mv | AT khalidfalrawi studyingtheeffectsofsomecompoundsandionsonescherichiacolialkalinephosphataseactivity |