Genetic characterization and population structure of six brown layer pure lines using microsatellite markers
Objective The first stage in both breeding and programs for the conservation of genetic resources are the identification of genetic diversity in the relevant population. The aim of the present study is to identify genetic diversity of six brown layer pure chicken lines (Rhode Island Red [RIRI, RIRII...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2019-01-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-17-0870.pdf |
| _version_ | 1818134370725134336 |
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| author | Taki Karsli Murat Soner Balcıoğlu |
| author_facet | Taki Karsli Murat Soner Balcıoğlu |
| author_sort | Taki Karsli |
| collection | DOAJ |
| description | Objective The first stage in both breeding and programs for the conservation of genetic resources are the identification of genetic diversity in the relevant population. The aim of the present study is to identify genetic diversity of six brown layer pure chicken lines (Rhode Island Red [RIRI, RIRII], Barred Rock [BARI, BARII], Columbian Rock [COL], and line 54 [L-54]) with microsatellite markers. Furthermore, the study aims to employ its findings to discuss the possibilities for the conservation and sustainable use of these lines that have been bred as closed populations for a long time. Methods In the present study, a total number of 180 samples belonging to RIRI (n = 30), RIRII (n = 30), BARI (n = 30), BARII (n = 30), L-54 (n = 30), and COL (n = 30) lines were genotyped using 22 microsatellite loci. Microsatellite markers are extremely useful tools in the identification of genetic diversity since they are distributed throughout the eukaryotic genome in multitudes, demonstrate co-dominant inheritance and they feature a high rate of polymorphism and repeatability. Results In this study, we found all loci to be polymorphic and identified the average number of alleles per locus to be in the range between 4.41 (BARI) and 5.45 (RIRI); the observed heterozygosity to be in the range between 0.31 (RIRII) and 0.50 (BARII); and FIS (inbreeding coefficient) values in the range between 0.16 (L-54) and 0.46 (RIRII). The FIS values obtained in this context points out to a deviation from Hardy-Weinberg equilibrium due to heterozygote deficiency in six different populations. The Neighbour-Joining tree, Factorial Correspondence Analysis and STRUCTURE clustering analyzes showed that six brown layer lines were separated according to their genetic origins. Conclusion The results obtained from the study indicate a medium level of genetic diversity, high level inbreeding in chicken lines and high level genetic differentiation between chicken lines. |
| first_indexed | 2024-12-11T09:07:33Z |
| format | Article |
| id | doaj.art-5df4aba488074679ad8dbf60de0596da |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-11T09:07:33Z |
| publishDate | 2019-01-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-5df4aba488074679ad8dbf60de0596da2022-12-22T01:13:35ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172019-01-01321495710.5713/ajas.17.087024149Genetic characterization and population structure of six brown layer pure lines using microsatellite markersTaki Karsli0Murat Soner Balcıoğlu1 Faculty of Agriculture, Department of Animal Science, Akdeniz University, Antalya 07058, Turkey Faculty of Agriculture, Department of Animal Science, Akdeniz University, Antalya 07058, TurkeyObjective The first stage in both breeding and programs for the conservation of genetic resources are the identification of genetic diversity in the relevant population. The aim of the present study is to identify genetic diversity of six brown layer pure chicken lines (Rhode Island Red [RIRI, RIRII], Barred Rock [BARI, BARII], Columbian Rock [COL], and line 54 [L-54]) with microsatellite markers. Furthermore, the study aims to employ its findings to discuss the possibilities for the conservation and sustainable use of these lines that have been bred as closed populations for a long time. Methods In the present study, a total number of 180 samples belonging to RIRI (n = 30), RIRII (n = 30), BARI (n = 30), BARII (n = 30), L-54 (n = 30), and COL (n = 30) lines were genotyped using 22 microsatellite loci. Microsatellite markers are extremely useful tools in the identification of genetic diversity since they are distributed throughout the eukaryotic genome in multitudes, demonstrate co-dominant inheritance and they feature a high rate of polymorphism and repeatability. Results In this study, we found all loci to be polymorphic and identified the average number of alleles per locus to be in the range between 4.41 (BARI) and 5.45 (RIRI); the observed heterozygosity to be in the range between 0.31 (RIRII) and 0.50 (BARII); and FIS (inbreeding coefficient) values in the range between 0.16 (L-54) and 0.46 (RIRII). The FIS values obtained in this context points out to a deviation from Hardy-Weinberg equilibrium due to heterozygote deficiency in six different populations. The Neighbour-Joining tree, Factorial Correspondence Analysis and STRUCTURE clustering analyzes showed that six brown layer lines were separated according to their genetic origins. Conclusion The results obtained from the study indicate a medium level of genetic diversity, high level inbreeding in chicken lines and high level genetic differentiation between chicken lines.http://www.ajas.info/upload/pdf/ajas-17-0870.pdfMicrosatelliteGenetic DiversityPopulation StructurePure Chicken Line |
| spellingShingle | Taki Karsli Murat Soner Balcıoğlu Genetic characterization and population structure of six brown layer pure lines using microsatellite markers Asian-Australasian Journal of Animal Sciences Microsatellite Genetic Diversity Population Structure Pure Chicken Line |
| title | Genetic characterization and population structure of six brown layer pure lines using microsatellite markers |
| title_full | Genetic characterization and population structure of six brown layer pure lines using microsatellite markers |
| title_fullStr | Genetic characterization and population structure of six brown layer pure lines using microsatellite markers |
| title_full_unstemmed | Genetic characterization and population structure of six brown layer pure lines using microsatellite markers |
| title_short | Genetic characterization and population structure of six brown layer pure lines using microsatellite markers |
| title_sort | genetic characterization and population structure of six brown layer pure lines using microsatellite markers |
| topic | Microsatellite Genetic Diversity Population Structure Pure Chicken Line |
| url | http://www.ajas.info/upload/pdf/ajas-17-0870.pdf |
| work_keys_str_mv | AT takikarsli geneticcharacterizationandpopulationstructureofsixbrownlayerpurelinesusingmicrosatellitemarkers AT muratsonerbalcıoglu geneticcharacterizationandpopulationstructureofsixbrownlayerpurelinesusingmicrosatellitemarkers |