The impact of antibiotic residues on resistance patterns in leek at harvest
When crops are cultivated on fields fertilized with animal manure, the risk exists that plants may take up antibiotic residues and may be exposed to antibiotic resistance genes and antibiotic resistant bacteria. During cultivation in a greenhouse pot experiment, leek (Allium porrum) was fertilized w...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023032590 |
| _version_ | 1797815570820759552 |
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| author | Judith Huygens Geertrui Rasschaert Bart Cottyn Jeroen Dewulf Els Van Coillie Koen Willekens Paul Quataert Ilse Becue Els Daeseleire Marc Heyndrickx |
| author_facet | Judith Huygens Geertrui Rasschaert Bart Cottyn Jeroen Dewulf Els Van Coillie Koen Willekens Paul Quataert Ilse Becue Els Daeseleire Marc Heyndrickx |
| author_sort | Judith Huygens |
| collection | DOAJ |
| description | When crops are cultivated on fields fertilized with animal manure, the risk exists that plants may take up antibiotic residues and may be exposed to antibiotic resistance genes and antibiotic resistant bacteria. During cultivation in a greenhouse pot experiment, leek (Allium porrum) was fertilized with either pig slurry or mineral fertilizer and exposed to either no antibiotics, doxycycline (10,000 μg/kg manure), sulfadiazine (1000 μg/kg manure), or lincomycin (1000 μg/kg manure). At harvest, 4.5 months later, lincomycin, sulfadiazine or doxycycline were not detected in any of the leek samples nor in their corresponding soil samples. Further, antimicrobial susceptibility testing was performed on 181 Bacillus cereus group isolates and 52 Pseudomonas aeruginosa isolates from the grown leek. For the B. cereus group isolates, only a small shift in MIC50 for lincomycin was observed among isolates from the lincomycin and control treatment. For P. aeruginosa, only in the setup with doxycycline treatment a higher MIC50 for doxycycline was observed compared to the control, specifically the isolates selected from growth media supplemented with 8 mg/L doxycycline. Nine antibiotic resistance genes (tet(B), tet(L), tet(M), tet(O), tet(Q), tet(W), erm(B), erm(F) and sul2) were investigated at harvest in the leek and soil samples. In the leek samples, none of the antibiotic resistance genes were detected. In the soil samples fertilized with pig slurry, the genes erm(B), erm(F), tet(M), sul2, tet(W) and tet(O) were detected in significantly higher copy numbers in the lincomycin treatment as compared to the other antibiotic treatments. This could be due to a shift in soil microbiota induced by the addition of lincomycin. The results of this study indicate that consumption of leek carries a low risk of exposure to antibiotic residues or antibiotic resistance to doxycycline, sulfadiazine or lincomycin. |
| first_indexed | 2024-03-13T08:24:49Z |
| format | Article |
| id | doaj.art-5df5b07e55714cd2b33dcaf44ba26d1f |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T08:24:49Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-5df5b07e55714cd2b33dcaf44ba26d1f2023-05-31T04:46:27ZengElsevierHeliyon2405-84402023-05-0195e16052The impact of antibiotic residues on resistance patterns in leek at harvestJudith Huygens0Geertrui Rasschaert1Bart Cottyn2Jeroen Dewulf3Els Van Coillie4Koen Willekens5Paul Quataert6Ilse Becue7Els Daeseleire8Marc Heyndrickx9Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, BelgiumFlanders Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, Belgium; Corresponding author.Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Plant Science Unit, Caritasstraat 39, 9090 Melle, BelgiumGhent University, Faculty of Veterinary Medicine, Department of Internal Medicine, Reproduction an Population Medicine, Salisburylaan 133, 9820 Merelbeke, BelgiumFlanders Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, BelgiumFlanders Research Institute for Agriculture, Fisheries and Food (ILVO), Plant Science Unit, Caritasstraat 39, 9090 Melle, BelgiumFlanders Research Institute for Agriculture, Fisheries and Food (ILVO), Plant Science Unit, Caritasstraat 39, 9090 Melle, BelgiumFlanders Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, BelgiumFlanders Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, BelgiumFlanders Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, Belgium; Ghent University, Faculty of Veterinary Medicine, Department of Pathobiology, Pharmacology and Zoological Medicine, Salisburylaan 133, 9820 Merelbeke, BelgiumWhen crops are cultivated on fields fertilized with animal manure, the risk exists that plants may take up antibiotic residues and may be exposed to antibiotic resistance genes and antibiotic resistant bacteria. During cultivation in a greenhouse pot experiment, leek (Allium porrum) was fertilized with either pig slurry or mineral fertilizer and exposed to either no antibiotics, doxycycline (10,000 μg/kg manure), sulfadiazine (1000 μg/kg manure), or lincomycin (1000 μg/kg manure). At harvest, 4.5 months later, lincomycin, sulfadiazine or doxycycline were not detected in any of the leek samples nor in their corresponding soil samples. Further, antimicrobial susceptibility testing was performed on 181 Bacillus cereus group isolates and 52 Pseudomonas aeruginosa isolates from the grown leek. For the B. cereus group isolates, only a small shift in MIC50 for lincomycin was observed among isolates from the lincomycin and control treatment. For P. aeruginosa, only in the setup with doxycycline treatment a higher MIC50 for doxycycline was observed compared to the control, specifically the isolates selected from growth media supplemented with 8 mg/L doxycycline. Nine antibiotic resistance genes (tet(B), tet(L), tet(M), tet(O), tet(Q), tet(W), erm(B), erm(F) and sul2) were investigated at harvest in the leek and soil samples. In the leek samples, none of the antibiotic resistance genes were detected. In the soil samples fertilized with pig slurry, the genes erm(B), erm(F), tet(M), sul2, tet(W) and tet(O) were detected in significantly higher copy numbers in the lincomycin treatment as compared to the other antibiotic treatments. This could be due to a shift in soil microbiota induced by the addition of lincomycin. The results of this study indicate that consumption of leek carries a low risk of exposure to antibiotic residues or antibiotic resistance to doxycycline, sulfadiazine or lincomycin.http://www.sciencedirect.com/science/article/pii/S2405844023032590Antibiotic residuesAntibiotic resistanceAntibiotic resistance genesAntibiotic resistant bacteriaManureSoil |
| spellingShingle | Judith Huygens Geertrui Rasschaert Bart Cottyn Jeroen Dewulf Els Van Coillie Koen Willekens Paul Quataert Ilse Becue Els Daeseleire Marc Heyndrickx The impact of antibiotic residues on resistance patterns in leek at harvest Heliyon Antibiotic residues Antibiotic resistance Antibiotic resistance genes Antibiotic resistant bacteria Manure Soil |
| title | The impact of antibiotic residues on resistance patterns in leek at harvest |
| title_full | The impact of antibiotic residues on resistance patterns in leek at harvest |
| title_fullStr | The impact of antibiotic residues on resistance patterns in leek at harvest |
| title_full_unstemmed | The impact of antibiotic residues on resistance patterns in leek at harvest |
| title_short | The impact of antibiotic residues on resistance patterns in leek at harvest |
| title_sort | impact of antibiotic residues on resistance patterns in leek at harvest |
| topic | Antibiotic residues Antibiotic resistance Antibiotic resistance genes Antibiotic resistant bacteria Manure Soil |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023032590 |
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