Prevalence of Common Respiratory Viral Infections and Identification of Adenovirus in Hospitalized Adults in Harbin, China 2014 to 2017
Background: Respiratory infections pose a great challenge in global health, and the prevalence of viral infection in adult patients has been poorly understood in northeast China. Harbin is one of the major cities in northeast China, and more than half of any given year in Harbin is occupied by winte...
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Frontiers Media S.A.
2018-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02919/full |
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| author | Yingchen Wang Tuo Dong Guiyun Qi Lixin Qu Wei Liang Binbin Qi Zhe Zhang Lei Shang Hong Gao Xiqiao Du Bing Lu Yan Guo Zhenwei Liu Huisong Yu Qi Cui Xiaocen Wang Ye Li Weiyuan Guo Zhangyi Qu Zhangyi Qu |
| author_facet | Yingchen Wang Tuo Dong Guiyun Qi Lixin Qu Wei Liang Binbin Qi Zhe Zhang Lei Shang Hong Gao Xiqiao Du Bing Lu Yan Guo Zhenwei Liu Huisong Yu Qi Cui Xiaocen Wang Ye Li Weiyuan Guo Zhangyi Qu Zhangyi Qu |
| author_sort | Yingchen Wang |
| collection | DOAJ |
| description | Background: Respiratory infections pose a great challenge in global health, and the prevalence of viral infection in adult patients has been poorly understood in northeast China. Harbin is one of the major cities in northeast China, and more than half of any given year in Harbin is occupied by winter. To reveal the viral etiology and seasonality in adult patients from Harbin, a 4-year consecutive survey was conducted in Harbin, China.Methods: From January 2014 to December 2017, specimens were obtained from adult patients admitted to the Second Affiliated Hospital of Harbin Medical University with lower respiratory tract infections. Sputum samples were examined by direct immunofluorescence assays to detect seven common respiratory viruses, including influenza virus (type A and B), parainfluenza virus (type 1 to 3), respiratory syncytial virus and adenovirus. Adenovirus positive samples were seeded onto A549 cells to isolate viral strains. Phylogenetic analysis was conducted on the highly variable region of adenoviral hexon gene.Results: A total of 1,300 hospitalized adult patients with lower respiratory tract infections were enrolled, in which 189 patients (14.5%) were detected as having at least one viral infection. The co-infection rate in this study was 25.9% (49/189). The dominant viral pathogen from 2014 to 2017 was parainfluenza virus, with a detection rate of 7.2%, followed by influenza virus, respiratory syncytial virus and adenovirus. Based on the climate seasons determined by daily average temperature, the highest overall viral detection rate was detected in spring (22.0%, 52/236), followed by winter (13.4%, 109/813), autumn (11.4%, 13/114) and summer (10.9%, 15/137). Adenovirus type 3 strains with slight variations were isolated from positive cases, which were closely related to the GB strain from the United States, as well as the Harbin04B strain isolated locally.Conclusion: This study demonstrated that common respiratory viruses were partially responsible for hospitalized lower respiratory tract infections in adult patients from Harbin, China, with parainfluenza virus as the dominant viral pathogen. Climate seasons could be rational indicators for the seasonality analysis of airborne viral infections. Future surveillance on viral mutations would be necessary to reveal the evolutionary history of respiratory viruses. |
| first_indexed | 2024-12-11T06:34:00Z |
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| id | doaj.art-5df6eece06db47dbaab082214eeb065d |
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| language | English |
| last_indexed | 2024-12-11T06:34:00Z |
| publishDate | 2018-11-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-5df6eece06db47dbaab082214eeb065d2022-12-22T01:17:26ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-11-01910.3389/fmicb.2018.02919411197Prevalence of Common Respiratory Viral Infections and Identification of Adenovirus in Hospitalized Adults in Harbin, China 2014 to 2017Yingchen Wang0Tuo Dong1Guiyun Qi2Lixin Qu3Wei Liang4Binbin Qi5Zhe Zhang6Lei Shang7Hong Gao8Xiqiao Du9Bing Lu10Yan Guo11Zhenwei Liu12Huisong Yu13Qi Cui14Xiaocen Wang15Ye Li16Weiyuan Guo17Zhangyi Qu18Zhangyi Qu19Department of Microbiology, Public Health College, Harbin Medical University, Harbin, ChinaDepartment of Microbiology, Public Health College, Harbin Medical University, Harbin, ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Microbiology, Public Health College, Harbin Medical University, Harbin, ChinaDepartment of Microbiology, Public Health College, Harbin Medical University, Harbin, ChinaDepartment of Microbiology, Public Health College, Harbin Medical University, Harbin, ChinaDepartment of Microbiology, Public Health College, Harbin Medical University, Harbin, ChinaDepartment of Microbiology, Public Health College, Harbin Medical University, Harbin, ChinaDepartment of Microbiology, Public Health College, Harbin Medical University, Harbin, ChinaDepartment of Microbiology, Public Health College, Harbin Medical University, Harbin, ChinaDepartment of Microbiology, Public Health College, Harbin Medical University, Harbin, ChinaDepartment of Ear Nose Throat, The Second Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Microbiology, Public Health College, Harbin Medical University, Harbin, ChinaDepartment of Microbiology, Public Health College, Harbin Medical University, Harbin, ChinaDepartment of Microbiology, Public Health College, Harbin Medical University, Harbin, ChinaDepartment of Microbiology, Public Health College, Harbin Medical University, Harbin, ChinaDepartment of Microbiology, Public Health College, Harbin Medical University, Harbin, ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Microbiology, Public Health College, Harbin Medical University, Harbin, ChinaDepartment of Natural Focus Disease Control, Institute of Environment-Associated Disease, Sino-Russia Joint Medical Research Center, Harbin Medical University, Harbin, ChinaBackground: Respiratory infections pose a great challenge in global health, and the prevalence of viral infection in adult patients has been poorly understood in northeast China. Harbin is one of the major cities in northeast China, and more than half of any given year in Harbin is occupied by winter. To reveal the viral etiology and seasonality in adult patients from Harbin, a 4-year consecutive survey was conducted in Harbin, China.Methods: From January 2014 to December 2017, specimens were obtained from adult patients admitted to the Second Affiliated Hospital of Harbin Medical University with lower respiratory tract infections. Sputum samples were examined by direct immunofluorescence assays to detect seven common respiratory viruses, including influenza virus (type A and B), parainfluenza virus (type 1 to 3), respiratory syncytial virus and adenovirus. Adenovirus positive samples were seeded onto A549 cells to isolate viral strains. Phylogenetic analysis was conducted on the highly variable region of adenoviral hexon gene.Results: A total of 1,300 hospitalized adult patients with lower respiratory tract infections were enrolled, in which 189 patients (14.5%) were detected as having at least one viral infection. The co-infection rate in this study was 25.9% (49/189). The dominant viral pathogen from 2014 to 2017 was parainfluenza virus, with a detection rate of 7.2%, followed by influenza virus, respiratory syncytial virus and adenovirus. Based on the climate seasons determined by daily average temperature, the highest overall viral detection rate was detected in spring (22.0%, 52/236), followed by winter (13.4%, 109/813), autumn (11.4%, 13/114) and summer (10.9%, 15/137). Adenovirus type 3 strains with slight variations were isolated from positive cases, which were closely related to the GB strain from the United States, as well as the Harbin04B strain isolated locally.Conclusion: This study demonstrated that common respiratory viruses were partially responsible for hospitalized lower respiratory tract infections in adult patients from Harbin, China, with parainfluenza virus as the dominant viral pathogen. Climate seasons could be rational indicators for the seasonality analysis of airborne viral infections. Future surveillance on viral mutations would be necessary to reveal the evolutionary history of respiratory viruses.https://www.frontiersin.org/article/10.3389/fmicb.2018.02919/fullhuman adenovirusrespiratory viral infectionslower respiratory tract infectionsadult patientsepidemiology |
| spellingShingle | Yingchen Wang Tuo Dong Guiyun Qi Lixin Qu Wei Liang Binbin Qi Zhe Zhang Lei Shang Hong Gao Xiqiao Du Bing Lu Yan Guo Zhenwei Liu Huisong Yu Qi Cui Xiaocen Wang Ye Li Weiyuan Guo Zhangyi Qu Zhangyi Qu Prevalence of Common Respiratory Viral Infections and Identification of Adenovirus in Hospitalized Adults in Harbin, China 2014 to 2017 Frontiers in Microbiology human adenovirus respiratory viral infections lower respiratory tract infections adult patients epidemiology |
| title | Prevalence of Common Respiratory Viral Infections and Identification of Adenovirus in Hospitalized Adults in Harbin, China 2014 to 2017 |
| title_full | Prevalence of Common Respiratory Viral Infections and Identification of Adenovirus in Hospitalized Adults in Harbin, China 2014 to 2017 |
| title_fullStr | Prevalence of Common Respiratory Viral Infections and Identification of Adenovirus in Hospitalized Adults in Harbin, China 2014 to 2017 |
| title_full_unstemmed | Prevalence of Common Respiratory Viral Infections and Identification of Adenovirus in Hospitalized Adults in Harbin, China 2014 to 2017 |
| title_short | Prevalence of Common Respiratory Viral Infections and Identification of Adenovirus in Hospitalized Adults in Harbin, China 2014 to 2017 |
| title_sort | prevalence of common respiratory viral infections and identification of adenovirus in hospitalized adults in harbin china 2014 to 2017 |
| topic | human adenovirus respiratory viral infections lower respiratory tract infections adult patients epidemiology |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.02919/full |
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