AKTIVITAS URIKASE YANG DIHASILKAN DARI BERBAGAI SEL LACTOBACILLUS PLANTARUM DAN PARAMETER KINETIKANYA
Uric acid concentration could be determined by spectrophotometry method. Uric acid was oxidized into allatonin in the presence of uricase and calculated by measuring the decrease of uric acid absorbance at 293 nm. These uricase were obtained from cells of Lactobacillus plantarum. L. plantarum K. Mar...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2009-12-01
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| Series: | Jurnal Ilmu Pertanian Indonesia |
| Subjects: | |
| Online Access: | http://journal.ipb.ac.id/index.php/JIPI/article/view/6520 |
| _version_ | 1818149942750871552 |
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| author | Dyah Iswantini Novik Nurhidayat Trivadila Eka Mardiah |
| author_facet | Dyah Iswantini Novik Nurhidayat Trivadila Eka Mardiah |
| author_sort | Dyah Iswantini |
| collection | DOAJ |
| description | Uric acid concentration could be determined by spectrophotometry method. Uric acid was oxidized into allatonin in the presence of uricase and calculated by measuring the decrease of uric acid absorbance at 293 nm. These uricase were obtained from cells of Lactobacillus plantarum. L. plantarum K. Mar. E was isolated from passion fruit skin and L. plantarum Mgs. Psmb and Mgs. Bst from mangosteen. This research was conducted to observe the activity and kinetics of uricase from various cells of L. plantarum by spectrophotometric method. The plate assay method indicated that L. plantarum produced uricase, based on the clear zone about 0,2 mm on glucose yeast peptone medium contained 0,2% uric acid. The optimum condition of uricase activity from the three different sources occured in physiological condition. Uricase activity generated from cells of L. plantarum K. Mar. E, Mgs. Psmb, and Mgs. Bst were 0,1073; 0,0867; and 0,0842 U/mL, respectively. The kinetic parameters for uricase, determined with uric acid as the substrate. Vmax produced by L. plantarum K. Mar. E, Mgs. Psmb, and Mgs. Bst were 1,3635; 0,0316; and 0,0418 U/mL of bacterial culture, respectively and KM 0,1541; 0,0061; and 0,0054 mM, respectively. Uricase activity in various bacterial cells of L. plantarum was stable until the second day |
| first_indexed | 2024-12-11T13:15:03Z |
| format | Article |
| id | doaj.art-5df80eaed2624ea3bc468910a5fd5d98 |
| institution | Directory Open Access Journal |
| issn | 0853-4217 2443-3462 |
| language | English |
| last_indexed | 2024-12-11T13:15:03Z |
| publishDate | 2009-12-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Jurnal Ilmu Pertanian Indonesia |
| spelling | doaj.art-5df80eaed2624ea3bc468910a5fd5d982022-12-22T01:06:04ZengBogor Agricultural UniversityJurnal Ilmu Pertanian Indonesia0853-42172443-34622009-12-01143163169AKTIVITAS URIKASE YANG DIHASILKAN DARI BERBAGAI SEL LACTOBACILLUS PLANTARUM DAN PARAMETER KINETIKANYADyah Iswantini0Novik Nurhidayat1Trivadila2Eka Mardiah3Departemen Kimia Fakultras Matematika dan IPA, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680Divisi Mikrobiologi R&D Biologi, Lembaga Ilmu Pengetahuan Indonesia, BogorDepartemen Kimia Fakultras Matematika dan IPA, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680Departemen Kimia Fakultras Matematika dan IPA, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680Uric acid concentration could be determined by spectrophotometry method. Uric acid was oxidized into allatonin in the presence of uricase and calculated by measuring the decrease of uric acid absorbance at 293 nm. These uricase were obtained from cells of Lactobacillus plantarum. L. plantarum K. Mar. E was isolated from passion fruit skin and L. plantarum Mgs. Psmb and Mgs. Bst from mangosteen. This research was conducted to observe the activity and kinetics of uricase from various cells of L. plantarum by spectrophotometric method. The plate assay method indicated that L. plantarum produced uricase, based on the clear zone about 0,2 mm on glucose yeast peptone medium contained 0,2% uric acid. The optimum condition of uricase activity from the three different sources occured in physiological condition. Uricase activity generated from cells of L. plantarum K. Mar. E, Mgs. Psmb, and Mgs. Bst were 0,1073; 0,0867; and 0,0842 U/mL, respectively. The kinetic parameters for uricase, determined with uric acid as the substrate. Vmax produced by L. plantarum K. Mar. E, Mgs. Psmb, and Mgs. Bst were 1,3635; 0,0316; and 0,0418 U/mL of bacterial culture, respectively and KM 0,1541; 0,0061; and 0,0054 mM, respectively. Uricase activity in various bacterial cells of L. plantarum was stable until the second dayhttp://journal.ipb.ac.id/index.php/JIPI/article/view/6520ActivityuricasespectrophotometrykineticsLactobacillus plantarum cells |
| spellingShingle | Dyah Iswantini Novik Nurhidayat Trivadila Eka Mardiah AKTIVITAS URIKASE YANG DIHASILKAN DARI BERBAGAI SEL LACTOBACILLUS PLANTARUM DAN PARAMETER KINETIKANYA Jurnal Ilmu Pertanian Indonesia Activity uricase spectrophotometry kinetics Lactobacillus plantarum cells |
| title | AKTIVITAS URIKASE YANG DIHASILKAN DARI BERBAGAI SEL LACTOBACILLUS PLANTARUM DAN PARAMETER KINETIKANYA |
| title_full | AKTIVITAS URIKASE YANG DIHASILKAN DARI BERBAGAI SEL LACTOBACILLUS PLANTARUM DAN PARAMETER KINETIKANYA |
| title_fullStr | AKTIVITAS URIKASE YANG DIHASILKAN DARI BERBAGAI SEL LACTOBACILLUS PLANTARUM DAN PARAMETER KINETIKANYA |
| title_full_unstemmed | AKTIVITAS URIKASE YANG DIHASILKAN DARI BERBAGAI SEL LACTOBACILLUS PLANTARUM DAN PARAMETER KINETIKANYA |
| title_short | AKTIVITAS URIKASE YANG DIHASILKAN DARI BERBAGAI SEL LACTOBACILLUS PLANTARUM DAN PARAMETER KINETIKANYA |
| title_sort | aktivitas urikase yang dihasilkan dari berbagai sel lactobacillus plantarum dan parameter kinetikanya |
| topic | Activity uricase spectrophotometry kinetics Lactobacillus plantarum cells |
| url | http://journal.ipb.ac.id/index.php/JIPI/article/view/6520 |
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