Toxicity and Biological Effects of <i>Beauveria brongniartii</i> Fe<sup>0</sup> Nanoparticles against <i>Spodoptera litura</i> (Fabricius)
Nanotechnology has clear potential in the development of innovative insecticidal products for the biorational management of major insect pests. Metal-based nanoparticles of different microbial pest control agents have been effective against several pests. Synthesis of <i>Beauveria brongniartii...
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MDPI AG
2020-12-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/12/895 |
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| author | Jing Xu Kaihui Zhang Andrew G. S. Cuthbertson Cailian Du Shaukat Ali |
| author_facet | Jing Xu Kaihui Zhang Andrew G. S. Cuthbertson Cailian Du Shaukat Ali |
| author_sort | Jing Xu |
| collection | DOAJ |
| description | Nanotechnology has clear potential in the development of innovative insecticidal products for the biorational management of major insect pests. Metal-based nanoparticles of different microbial pest control agents have been effective against several pests. Synthesis of <i>Beauveria brongniartii</i> based Fe<sup>0</sup> nanoparticles (Fe<sup>0</sup>NPs) and their bio-efficacy against <i>Spodoptera litura</i> was observed during this study. <i>Beauveria brongniartii</i> conidia were coated with Fe<sup>0</sup>NPs and characterized by applying a selection of different analytical techniques. Ultraviolet (UV) spectroscopy showed the characteristic band of surface plasmon at 430 nm; Scanning electron microscopy (SEM) images showed spherical shaped nanoparticles with a size ranging between 0.41 to 0.80 µm; Energy-dispersive X-ray (EDX) spectral analysis revealed characteristic Fe peaks at 6.5 and 7.1 Kev; the X-ray diffractogram showed three strong peaks at 2<i>θ</i> values of 45.72°, 64.47°, and 84.05°. The bioassay studies demonstrated that mortality of 2nd instar <i>S. litura</i> larvae following Fe<sup>0</sup>NPs treatment increased with increasing concentrations of Fe<sup>0</sup>NPs at different time intervals. The median lethal concentration (LC<sub>50</sub>) values of Fe<sup>0</sup>NPs against <i>S. litura</i> after seven days of fungal treatment was 59 ppm, whereas median survival time (LT<sub>50</sub>) values for 200 and 500 ppm concentrations of Fe<sup>0</sup>NPs against <i>S. litura</i> seven days post-treatment were 5.1 and 2.29 days, respectively. <i>Beauveria brongniartii</i>-Fe<sup>0</sup>NPs caused significant reductions in feeding and growth parameters (relative growth rate, relative consumption rate, and efficiency of conversion of ingested food) of <i>S. litura</i>. <i>Beauveria brongniartii</i> Fe<sup>0</sup>NPs induced reduction in glutathione-S-transferase activities throughout the infection period whereas activities of antioxidant enzymes decreased during later periods of infection. These findings suggest that <i>B. brongniartii</i> Fe<sup>0</sup>NPs can potentially be used in biorational <i>S. litura</i> management programs. |
| first_indexed | 2024-03-10T13:53:26Z |
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| language | English |
| last_indexed | 2024-03-10T13:53:26Z |
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| spelling | doaj.art-5df9ef308d224da1a6e7c1f3c280c4292023-11-21T01:53:44ZengMDPI AGInsects2075-44502020-12-01111289510.3390/insects11120895Toxicity and Biological Effects of <i>Beauveria brongniartii</i> Fe<sup>0</sup> Nanoparticles against <i>Spodoptera litura</i> (Fabricius)Jing Xu0Kaihui Zhang1Andrew G. S. Cuthbertson2Cailian Du3Shaukat Ali4Key Laboratory of Bio-Pesticide Innovation and Application, Guangzhou 510642, ChinaKey Laboratory of Bio-Pesticide Innovation and Application, Guangzhou 510642, ChinaIndependent Science Advisor, York YO10 5AQ, UKKey Laboratory of Bio-Pesticide Innovation and Application, Guangzhou 510642, ChinaKey Laboratory of Bio-Pesticide Innovation and Application, Guangzhou 510642, ChinaNanotechnology has clear potential in the development of innovative insecticidal products for the biorational management of major insect pests. Metal-based nanoparticles of different microbial pest control agents have been effective against several pests. Synthesis of <i>Beauveria brongniartii</i> based Fe<sup>0</sup> nanoparticles (Fe<sup>0</sup>NPs) and their bio-efficacy against <i>Spodoptera litura</i> was observed during this study. <i>Beauveria brongniartii</i> conidia were coated with Fe<sup>0</sup>NPs and characterized by applying a selection of different analytical techniques. Ultraviolet (UV) spectroscopy showed the characteristic band of surface plasmon at 430 nm; Scanning electron microscopy (SEM) images showed spherical shaped nanoparticles with a size ranging between 0.41 to 0.80 µm; Energy-dispersive X-ray (EDX) spectral analysis revealed characteristic Fe peaks at 6.5 and 7.1 Kev; the X-ray diffractogram showed three strong peaks at 2<i>θ</i> values of 45.72°, 64.47°, and 84.05°. The bioassay studies demonstrated that mortality of 2nd instar <i>S. litura</i> larvae following Fe<sup>0</sup>NPs treatment increased with increasing concentrations of Fe<sup>0</sup>NPs at different time intervals. The median lethal concentration (LC<sub>50</sub>) values of Fe<sup>0</sup>NPs against <i>S. litura</i> after seven days of fungal treatment was 59 ppm, whereas median survival time (LT<sub>50</sub>) values for 200 and 500 ppm concentrations of Fe<sup>0</sup>NPs against <i>S. litura</i> seven days post-treatment were 5.1 and 2.29 days, respectively. <i>Beauveria brongniartii</i>-Fe<sup>0</sup>NPs caused significant reductions in feeding and growth parameters (relative growth rate, relative consumption rate, and efficiency of conversion of ingested food) of <i>S. litura</i>. <i>Beauveria brongniartii</i> Fe<sup>0</sup>NPs induced reduction in glutathione-S-transferase activities throughout the infection period whereas activities of antioxidant enzymes decreased during later periods of infection. These findings suggest that <i>B. brongniartii</i> Fe<sup>0</sup>NPs can potentially be used in biorational <i>S. litura</i> management programs.https://www.mdpi.com/2075-4450/11/12/895biological controlentomopathogenic fungusfeedinggrowthtoxicity |
| spellingShingle | Jing Xu Kaihui Zhang Andrew G. S. Cuthbertson Cailian Du Shaukat Ali Toxicity and Biological Effects of <i>Beauveria brongniartii</i> Fe<sup>0</sup> Nanoparticles against <i>Spodoptera litura</i> (Fabricius) Insects biological control entomopathogenic fungus feeding growth toxicity |
| title | Toxicity and Biological Effects of <i>Beauveria brongniartii</i> Fe<sup>0</sup> Nanoparticles against <i>Spodoptera litura</i> (Fabricius) |
| title_full | Toxicity and Biological Effects of <i>Beauveria brongniartii</i> Fe<sup>0</sup> Nanoparticles against <i>Spodoptera litura</i> (Fabricius) |
| title_fullStr | Toxicity and Biological Effects of <i>Beauveria brongniartii</i> Fe<sup>0</sup> Nanoparticles against <i>Spodoptera litura</i> (Fabricius) |
| title_full_unstemmed | Toxicity and Biological Effects of <i>Beauveria brongniartii</i> Fe<sup>0</sup> Nanoparticles against <i>Spodoptera litura</i> (Fabricius) |
| title_short | Toxicity and Biological Effects of <i>Beauveria brongniartii</i> Fe<sup>0</sup> Nanoparticles against <i>Spodoptera litura</i> (Fabricius) |
| title_sort | toxicity and biological effects of i beauveria brongniartii i fe sup 0 sup nanoparticles against i spodoptera litura i fabricius |
| topic | biological control entomopathogenic fungus feeding growth toxicity |
| url | https://www.mdpi.com/2075-4450/11/12/895 |
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