Synthetic mRNA capping
Eukaryotic mRNA with its 5′-cap is of central importance for the cell. Many studies involving mRNA require reliable preparation and modification of 5′-capped RNAs. Depending on the length of the desired capped RNA, chemical or enzymatic preparation – or a combination of both – can be advantageous. W...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Beilstein-Institut
2017-12-01
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| Series: | Beilstein Journal of Organic Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.3762/bjoc.13.274 |
| _version_ | 1818617785131991040 |
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| author | Fabian Muttach Nils Muthmann Andrea Rentmeister |
| author_facet | Fabian Muttach Nils Muthmann Andrea Rentmeister |
| author_sort | Fabian Muttach |
| collection | DOAJ |
| description | Eukaryotic mRNA with its 5′-cap is of central importance for the cell. Many studies involving mRNA require reliable preparation and modification of 5′-capped RNAs. Depending on the length of the desired capped RNA, chemical or enzymatic preparation – or a combination of both – can be advantageous. We review state-of-the art methods and give directions for choosing the appropriate approach. We also discuss the preparation and properties of mRNAs with non-natural caps providing novel features such as improved stability or enhanced translational efficiency. |
| first_indexed | 2024-12-16T17:11:13Z |
| format | Article |
| id | doaj.art-5dfbdefcfc5643d8958c0a4e721c52d3 |
| institution | Directory Open Access Journal |
| issn | 1860-5397 |
| language | English |
| last_indexed | 2024-12-16T17:11:13Z |
| publishDate | 2017-12-01 |
| publisher | Beilstein-Institut |
| record_format | Article |
| series | Beilstein Journal of Organic Chemistry |
| spelling | doaj.art-5dfbdefcfc5643d8958c0a4e721c52d32022-12-21T22:23:24ZengBeilstein-InstitutBeilstein Journal of Organic Chemistry1860-53972017-12-011312819283210.3762/bjoc.13.2741860-5397-13-274Synthetic mRNA cappingFabian Muttach0Nils Muthmann1Andrea Rentmeister2University of Münster, Department of Chemistry, Institute of Biochemistry, Wilhelm-Klemm-Str. 2, 48149 Münster, GermanyUniversity of Münster, Department of Chemistry, Institute of Biochemistry, Wilhelm-Klemm-Str. 2, 48149 Münster, GermanyUniversity of Münster, Department of Chemistry, Institute of Biochemistry, Wilhelm-Klemm-Str. 2, 48149 Münster, GermanyEukaryotic mRNA with its 5′-cap is of central importance for the cell. Many studies involving mRNA require reliable preparation and modification of 5′-capped RNAs. Depending on the length of the desired capped RNA, chemical or enzymatic preparation – or a combination of both – can be advantageous. We review state-of-the art methods and give directions for choosing the appropriate approach. We also discuss the preparation and properties of mRNAs with non-natural caps providing novel features such as improved stability or enhanced translational efficiency.https://doi.org/10.3762/bjoc.13.274cap analoguecap synthesisclick chemistryenzymatic cappingmethyltransferaseRNA |
| spellingShingle | Fabian Muttach Nils Muthmann Andrea Rentmeister Synthetic mRNA capping Beilstein Journal of Organic Chemistry cap analogue cap synthesis click chemistry enzymatic capping methyltransferase RNA |
| title | Synthetic mRNA capping |
| title_full | Synthetic mRNA capping |
| title_fullStr | Synthetic mRNA capping |
| title_full_unstemmed | Synthetic mRNA capping |
| title_short | Synthetic mRNA capping |
| title_sort | synthetic mrna capping |
| topic | cap analogue cap synthesis click chemistry enzymatic capping methyltransferase RNA |
| url | https://doi.org/10.3762/bjoc.13.274 |
| work_keys_str_mv | AT fabianmuttach syntheticmrnacapping AT nilsmuthmann syntheticmrnacapping AT andrearentmeister syntheticmrnacapping |