Salvia miltiorrhiza polysaccharides ameliorates Staphylococcus aureus-induced mastitis in rats by inhibiting activation of the NF-κB and MAPK signaling pathways
Abstract The lactation capacity of dairy cows is critical to the productivity of the animals. Mastitis is a disease that directly affects the lactation capacity of cows. Staphylococcus aureus (S. aureus) is one of the most important pathogens that causes mastitis in dairy cows. The anti-inflammatory...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-05-01
|
| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12917-022-03312-6 |
| _version_ | 1818239290359939072 |
|---|---|
| author | Di Zhang Guozhong Jin Wei Liu Mengmeng Dou Xiao Wang Wanyu Shi Yongzhan Bao |
| author_facet | Di Zhang Guozhong Jin Wei Liu Mengmeng Dou Xiao Wang Wanyu Shi Yongzhan Bao |
| author_sort | Di Zhang |
| collection | DOAJ |
| description | Abstract The lactation capacity of dairy cows is critical to the productivity of the animals. Mastitis is a disease that directly affects the lactation capacity of cows. Staphylococcus aureus (S. aureus) is one of the most important pathogens that causes mastitis in dairy cows. The anti-inflammatory effect of Salvia miltiorrhiza polysaccharides (SMPs) has been demonstrated in mice and chickens. However, the effectiveness of SMPs in preventing and treating mastitis is unclear. Therefore, the purpose of this study was to explore the protective effect and mechanism of SMPs on mastitis caused by S. aureus. S. aureus was used to induce mastitis in rats, and three doses of SMPs (87.5, 175, 350 mg/kg, BW/d) were administered as treatments. The bacterial load, histopathology, and myeloperoxidase (MPO) and N-acetyl-β-D-glucosaminidase (NAGase) activities of mammary glands were observed and measured. Cytokines, including interleukin (IL)-1β, interleukin (IL)-6, and tumor necrosis factor α (TNF-α), were examined by qRT–PCR and ELISA. Key proteins in the NF-κB and MAPK signaling pathways were analyzed by Western blotting. The results showed that SMP supplementation could significantly reduce the colonization of S. aureus and the recruitment of inflammatory cells in mammary glands. S. aureus-induced gene transcription and protein expression of IL-1β, IL-6, and TNF-α were significantly suppressed in mammary glands. In addition, the increase in NF-κB and MAPK protein phosphorylation was inhibited by SMPs. These results revealed that supplementation with SMPs protected the mammary gland of rats against damage caused by S. aureus and alleviated the inflammatory response. This study provides a certain experimental basis for the treatment of S. aureus-induced mastitis with SMPs in the future. |
| first_indexed | 2024-12-12T12:55:12Z |
| format | Article |
| id | doaj.art-5dfc0b62348847ec8eb0d80f6bf9f06f |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-12T12:55:12Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-5dfc0b62348847ec8eb0d80f6bf9f06f2022-12-22T00:23:54ZengBMCBMC Veterinary Research1746-61482022-05-0118111110.1186/s12917-022-03312-6Salvia miltiorrhiza polysaccharides ameliorates Staphylococcus aureus-induced mastitis in rats by inhibiting activation of the NF-κB and MAPK signaling pathwaysDi Zhang0Guozhong Jin1Wei Liu2Mengmeng Dou3Xiao Wang4Wanyu Shi5Yongzhan Bao6College of Traditional Chinese Veterinary Medicine, Hebei Agricultural UniversityCollege of Traditional Chinese Veterinary Medicine, Hebei Agricultural UniversityCollege of Traditional Chinese Veterinary Medicine, Hebei Agricultural UniversityCollege of Traditional Chinese Veterinary Medicine, Hebei Agricultural UniversityCollege of Traditional Chinese Veterinary Medicine, Hebei Agricultural UniversityCollege of Traditional Chinese Veterinary Medicine, Hebei Agricultural UniversityCollege of Traditional Chinese Veterinary Medicine, Hebei Agricultural UniversityAbstract The lactation capacity of dairy cows is critical to the productivity of the animals. Mastitis is a disease that directly affects the lactation capacity of cows. Staphylococcus aureus (S. aureus) is one of the most important pathogens that causes mastitis in dairy cows. The anti-inflammatory effect of Salvia miltiorrhiza polysaccharides (SMPs) has been demonstrated in mice and chickens. However, the effectiveness of SMPs in preventing and treating mastitis is unclear. Therefore, the purpose of this study was to explore the protective effect and mechanism of SMPs on mastitis caused by S. aureus. S. aureus was used to induce mastitis in rats, and three doses of SMPs (87.5, 175, 350 mg/kg, BW/d) were administered as treatments. The bacterial load, histopathology, and myeloperoxidase (MPO) and N-acetyl-β-D-glucosaminidase (NAGase) activities of mammary glands were observed and measured. Cytokines, including interleukin (IL)-1β, interleukin (IL)-6, and tumor necrosis factor α (TNF-α), were examined by qRT–PCR and ELISA. Key proteins in the NF-κB and MAPK signaling pathways were analyzed by Western blotting. The results showed that SMP supplementation could significantly reduce the colonization of S. aureus and the recruitment of inflammatory cells in mammary glands. S. aureus-induced gene transcription and protein expression of IL-1β, IL-6, and TNF-α were significantly suppressed in mammary glands. In addition, the increase in NF-κB and MAPK protein phosphorylation was inhibited by SMPs. These results revealed that supplementation with SMPs protected the mammary gland of rats against damage caused by S. aureus and alleviated the inflammatory response. This study provides a certain experimental basis for the treatment of S. aureus-induced mastitis with SMPs in the future.https://doi.org/10.1186/s12917-022-03312-6Salvia miltiorrhiza polysaccharidesStaphylococcus aureusMastitisNF-κBMAPK |
| spellingShingle | Di Zhang Guozhong Jin Wei Liu Mengmeng Dou Xiao Wang Wanyu Shi Yongzhan Bao Salvia miltiorrhiza polysaccharides ameliorates Staphylococcus aureus-induced mastitis in rats by inhibiting activation of the NF-κB and MAPK signaling pathways BMC Veterinary Research Salvia miltiorrhiza polysaccharides Staphylococcus aureus Mastitis NF-κB MAPK |
| title | Salvia miltiorrhiza polysaccharides ameliorates Staphylococcus aureus-induced mastitis in rats by inhibiting activation of the NF-κB and MAPK signaling pathways |
| title_full | Salvia miltiorrhiza polysaccharides ameliorates Staphylococcus aureus-induced mastitis in rats by inhibiting activation of the NF-κB and MAPK signaling pathways |
| title_fullStr | Salvia miltiorrhiza polysaccharides ameliorates Staphylococcus aureus-induced mastitis in rats by inhibiting activation of the NF-κB and MAPK signaling pathways |
| title_full_unstemmed | Salvia miltiorrhiza polysaccharides ameliorates Staphylococcus aureus-induced mastitis in rats by inhibiting activation of the NF-κB and MAPK signaling pathways |
| title_short | Salvia miltiorrhiza polysaccharides ameliorates Staphylococcus aureus-induced mastitis in rats by inhibiting activation of the NF-κB and MAPK signaling pathways |
| title_sort | salvia miltiorrhiza polysaccharides ameliorates staphylococcus aureus induced mastitis in rats by inhibiting activation of the nf κb and mapk signaling pathways |
| topic | Salvia miltiorrhiza polysaccharides Staphylococcus aureus Mastitis NF-κB MAPK |
| url | https://doi.org/10.1186/s12917-022-03312-6 |
| work_keys_str_mv | AT dizhang salviamiltiorrhizapolysaccharidesamelioratesstaphylococcusaureusinducedmastitisinratsbyinhibitingactivationofthenfkbandmapksignalingpathways AT guozhongjin salviamiltiorrhizapolysaccharidesamelioratesstaphylococcusaureusinducedmastitisinratsbyinhibitingactivationofthenfkbandmapksignalingpathways AT weiliu salviamiltiorrhizapolysaccharidesamelioratesstaphylococcusaureusinducedmastitisinratsbyinhibitingactivationofthenfkbandmapksignalingpathways AT mengmengdou salviamiltiorrhizapolysaccharidesamelioratesstaphylococcusaureusinducedmastitisinratsbyinhibitingactivationofthenfkbandmapksignalingpathways AT xiaowang salviamiltiorrhizapolysaccharidesamelioratesstaphylococcusaureusinducedmastitisinratsbyinhibitingactivationofthenfkbandmapksignalingpathways AT wanyushi salviamiltiorrhizapolysaccharidesamelioratesstaphylococcusaureusinducedmastitisinratsbyinhibitingactivationofthenfkbandmapksignalingpathways AT yongzhanbao salviamiltiorrhizapolysaccharidesamelioratesstaphylococcusaureusinducedmastitisinratsbyinhibitingactivationofthenfkbandmapksignalingpathways |