Overexpression of GmBIN2, a soybean glycogen synthase kinase 3 gene, enhances tolerance to salt and drought in transgenic Arabidopsis and soybean hairy roots
Glycogen synthase kinase 3 (GSK3) is a kind of serine/threonine kinase widely found in eukaryotes. Many plant GSK3 kinases play important roles in regulating stress responses. This study investigated BRASSINOSTEROID-INSENSITIVE 2 (GmBIN2) gene, a member of the GSK3 protein kinase family in soybean a...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-09-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S209531191761863X |
| _version_ | 1818602852595007488 |
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| author | Ling-shuang WANG Qing-shan CHEN Da-wei XIN Zhao-ming QI Chao ZHANG Si-nan LI Yang-mei JIN Mo LI Hong-yao MEI An-yu SU Xiao-xia WU |
| author_facet | Ling-shuang WANG Qing-shan CHEN Da-wei XIN Zhao-ming QI Chao ZHANG Si-nan LI Yang-mei JIN Mo LI Hong-yao MEI An-yu SU Xiao-xia WU |
| author_sort | Ling-shuang WANG |
| collection | DOAJ |
| description | Glycogen synthase kinase 3 (GSK3) is a kind of serine/threonine kinase widely found in eukaryotes. Many plant GSK3 kinases play important roles in regulating stress responses. This study investigated BRASSINOSTEROID-INSENSITIVE 2 (GmBIN2) gene, a member of the GSK3 protein kinase family in soybean and an orthologue of Arabidopsis BIN2/AtSK21. GmBIN2 expression was increased by salt and drought stresses, but was not significantly affected by the ABA treatment. To examine the function of GmBIN2, transgenic Arabidopsis and transgenic soybean hairy roots were generated. Overexpression of GmBIN2 in Arabidopsis resulted in increased germination rate and root length compared with wild-type plants under salt and mannitol treatments. Overexpression of GmBIN2 increased cellular Ca2+ content and reduced Na+ content, enhancing salt tolerance in transgenic Arabidopsis plants. In the soybean hairy root assay, overexpression of GmBIN2 in transgenic roots also showed significantly higher relative root growth rate than the control when subjected to salt and mannitol treatments. Measurement of physiological indicators, including proline content, superoxide dismutase (SOD) activity, and relative electrical conductivity, supported this conclusion. Furthermore, we also found that GmBIN2 could up-regulate the expression of some stress-related genes in transgenic Arabidopsis and soybean hairy roots. Overall, these results indicated that GmBIN2 improved tolerance to salt and drought in transgenic Arabidopsis and soybean hairy roots. |
| first_indexed | 2024-12-16T13:13:52Z |
| format | Article |
| id | doaj.art-5dfcb6b7cd794b12b73b68d0a309c3cf |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-16T13:13:52Z |
| publishDate | 2018-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-5dfcb6b7cd794b12b73b68d0a309c3cf2022-12-21T22:30:31ZengElsevierJournal of Integrative Agriculture2095-31192018-09-0117919591971Overexpression of GmBIN2, a soybean glycogen synthase kinase 3 gene, enhances tolerance to salt and drought in transgenic Arabidopsis and soybean hairy rootsLing-shuang WANG0Qing-shan CHEN1Da-wei XIN2Zhao-ming QI3Chao ZHANG4Si-nan LI5Yang-mei JIN6Mo LI7Hong-yao MEI8An-yu SU9Xiao-xia WU10College of Agriculture, Northeast Agricultural University/Key Laboratory of Soybean Biology, Ministry of Education, Harbin 150030, P.R.ChinaCollege of Agriculture, Northeast Agricultural University/Key Laboratory of Soybean Biology, Ministry of Education, Harbin 150030, P.R.ChinaCollege of Agriculture, Northeast Agricultural University/Key Laboratory of Soybean Biology, Ministry of Education, Harbin 150030, P.R.ChinaCollege of Agriculture, Northeast Agricultural University/Key Laboratory of Soybean Biology, Ministry of Education, Harbin 150030, P.R.ChinaCollege of Agriculture, Northeast Agricultural University/Key Laboratory of Soybean Biology, Ministry of Education, Harbin 150030, P.R.ChinaCollege of Agriculture, Northeast Agricultural University/Key Laboratory of Soybean Biology, Ministry of Education, Harbin 150030, P.R.ChinaCollege of Agriculture, Northeast Agricultural University/Key Laboratory of Soybean Biology, Ministry of Education, Harbin 150030, P.R.ChinaCollege of Agriculture, Northeast Agricultural University/Key Laboratory of Soybean Biology, Ministry of Education, Harbin 150030, P.R.ChinaCollege of Agriculture, Northeast Agricultural University/Key Laboratory of Soybean Biology, Ministry of Education, Harbin 150030, P.R.ChinaCollege of Resources and Environment, Northeast Agricultural University, Harbin 150030, P.R.China; SU An-yu, Mobile: +86-13703631001, Fax: +86-451-55190447College of Agriculture, Northeast Agricultural University/Key Laboratory of Soybean Biology, Ministry of Education, Harbin 150030, P.R.China; Correspondence WU Xiao-xia, Tel: +86-451-55191642, Fax: +86-451-55190447Glycogen synthase kinase 3 (GSK3) is a kind of serine/threonine kinase widely found in eukaryotes. Many plant GSK3 kinases play important roles in regulating stress responses. This study investigated BRASSINOSTEROID-INSENSITIVE 2 (GmBIN2) gene, a member of the GSK3 protein kinase family in soybean and an orthologue of Arabidopsis BIN2/AtSK21. GmBIN2 expression was increased by salt and drought stresses, but was not significantly affected by the ABA treatment. To examine the function of GmBIN2, transgenic Arabidopsis and transgenic soybean hairy roots were generated. Overexpression of GmBIN2 in Arabidopsis resulted in increased germination rate and root length compared with wild-type plants under salt and mannitol treatments. Overexpression of GmBIN2 increased cellular Ca2+ content and reduced Na+ content, enhancing salt tolerance in transgenic Arabidopsis plants. In the soybean hairy root assay, overexpression of GmBIN2 in transgenic roots also showed significantly higher relative root growth rate than the control when subjected to salt and mannitol treatments. Measurement of physiological indicators, including proline content, superoxide dismutase (SOD) activity, and relative electrical conductivity, supported this conclusion. Furthermore, we also found that GmBIN2 could up-regulate the expression of some stress-related genes in transgenic Arabidopsis and soybean hairy roots. Overall, these results indicated that GmBIN2 improved tolerance to salt and drought in transgenic Arabidopsis and soybean hairy roots.http://www.sciencedirect.com/science/article/pii/S209531191761863XGmBIN2 geneabiotic stresstransgenic Arabidopsissoybean hairy roots |
| spellingShingle | Ling-shuang WANG Qing-shan CHEN Da-wei XIN Zhao-ming QI Chao ZHANG Si-nan LI Yang-mei JIN Mo LI Hong-yao MEI An-yu SU Xiao-xia WU Overexpression of GmBIN2, a soybean glycogen synthase kinase 3 gene, enhances tolerance to salt and drought in transgenic Arabidopsis and soybean hairy roots Journal of Integrative Agriculture GmBIN2 gene abiotic stress transgenic Arabidopsis soybean hairy roots |
| title | Overexpression of GmBIN2, a soybean glycogen synthase kinase 3 gene, enhances tolerance to salt and drought in transgenic Arabidopsis and soybean hairy roots |
| title_full | Overexpression of GmBIN2, a soybean glycogen synthase kinase 3 gene, enhances tolerance to salt and drought in transgenic Arabidopsis and soybean hairy roots |
| title_fullStr | Overexpression of GmBIN2, a soybean glycogen synthase kinase 3 gene, enhances tolerance to salt and drought in transgenic Arabidopsis and soybean hairy roots |
| title_full_unstemmed | Overexpression of GmBIN2, a soybean glycogen synthase kinase 3 gene, enhances tolerance to salt and drought in transgenic Arabidopsis and soybean hairy roots |
| title_short | Overexpression of GmBIN2, a soybean glycogen synthase kinase 3 gene, enhances tolerance to salt and drought in transgenic Arabidopsis and soybean hairy roots |
| title_sort | overexpression of gmbin2 a soybean glycogen synthase kinase 3 gene enhances tolerance to salt and drought in transgenic arabidopsis and soybean hairy roots |
| topic | GmBIN2 gene abiotic stress transgenic Arabidopsis soybean hairy roots |
| url | http://www.sciencedirect.com/science/article/pii/S209531191761863X |
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