Synthesis and Characterization of Nanofunctionalized Gelatin Methacrylate Hydrogels
Given the importance of the extracellular medium during tissue formation, it was wise to develop an artificial structure that mimics the extracellular matrix while having improved physico-chemical properties. That is why the choice was focused on gelatin methacryloyl (GelMA), an inexpensive biocompa...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/18/12/2675 |
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| author | Kamel Rahali Ghazi Ben Messaoud Cyril J.F. Kahn Laura Sanchez-Gonzalez Mouna Kaci Franck Cleymand Solenne Fleutot Michel Linder Stéphane Desobry Elmira Arab-Tehrany |
| author_facet | Kamel Rahali Ghazi Ben Messaoud Cyril J.F. Kahn Laura Sanchez-Gonzalez Mouna Kaci Franck Cleymand Solenne Fleutot Michel Linder Stéphane Desobry Elmira Arab-Tehrany |
| author_sort | Kamel Rahali |
| collection | DOAJ |
| description | Given the importance of the extracellular medium during tissue formation, it was wise to develop an artificial structure that mimics the extracellular matrix while having improved physico-chemical properties. That is why the choice was focused on gelatin methacryloyl (GelMA), an inexpensive biocompatible hydrogel. Physicochemical and mechanical properties were improved by the incorporation of nanoparticles developed from two innovative fabrication processes: High shear fluid and low frequencies/high frequencies ultrasounds. Both rapeseed nanoliposomes and nanodroplets were successfully incorporated in the GelMA networks during the photo polymerization process. The impact on polymer microstructure was investigated by Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and enzymatic degradation investigations. Mechanical stability and viscoelastic tests were conducted to demonstrate the beneficial effect of the functionalization on GelMA hydrogels. Adding nanoparticles to GelMA improved the surface properties (porosity), tuned swelling, and degradability properties. In addition, we observed that nanoemulsion didn’t change significantly the mechanical properties to shear and compression solicitations, whereas nanoliposome addition decreased Young’s modulus under compression solicitations. Thus, these ways of functionalization allow controlling the design of the material by choosing the type of nanoparticle (nanoliposome or nanoemulsion) in function of the application. |
| first_indexed | 2024-04-13T01:44:11Z |
| format | Article |
| id | doaj.art-5e011082e8ad4d1f99e907bd19690e07 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T01:44:11Z |
| publishDate | 2017-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5e011082e8ad4d1f99e907bd19690e072022-12-22T03:08:05ZengMDPI AGInternational Journal of Molecular Sciences1422-00672017-12-011812267510.3390/ijms18122675ijms18122675Synthesis and Characterization of Nanofunctionalized Gelatin Methacrylate HydrogelsKamel Rahali0Ghazi Ben Messaoud1Cyril J.F. Kahn2Laura Sanchez-Gonzalez3Mouna Kaci4Franck Cleymand5Solenne Fleutot6Michel Linder7Stéphane Desobry8Elmira Arab-Tehrany9Laboratoire d’Ingénierie des Biomolécules (LIBio), Université de Lorraine, 2 Avenue de la Forêt de Haye–BP 20163, 54505 Vandoeuvre-lès-Nancy, FranceLaboratoire d’Ingénierie des Biomolécules (LIBio), Université de Lorraine, 2 Avenue de la Forêt de Haye–BP 20163, 54505 Vandoeuvre-lès-Nancy, FranceLaboratoire d’Ingénierie des Biomolécules (LIBio), Université de Lorraine, 2 Avenue de la Forêt de Haye–BP 20163, 54505 Vandoeuvre-lès-Nancy, FranceLaboratoire d’Ingénierie des Biomolécules (LIBio), Université de Lorraine, 2 Avenue de la Forêt de Haye–BP 20163, 54505 Vandoeuvre-lès-Nancy, FranceLaboratoire d’Ingénierie des Biomolécules (LIBio), Université de Lorraine, 2 Avenue de la Forêt de Haye–BP 20163, 54505 Vandoeuvre-lès-Nancy, FranceInstitut Jean Lamour (UMR CNRS 7198), Université de Lorraine, Parc de Saurupt, CS 50840, 54011 Nancy CEDEX, FranceInstitut Jean Lamour (UMR CNRS 7198), Université de Lorraine, Parc de Saurupt, CS 50840, 54011 Nancy CEDEX, FranceLaboratoire d’Ingénierie des Biomolécules (LIBio), Université de Lorraine, 2 Avenue de la Forêt de Haye–BP 20163, 54505 Vandoeuvre-lès-Nancy, FranceLaboratoire d’Ingénierie des Biomolécules (LIBio), Université de Lorraine, 2 Avenue de la Forêt de Haye–BP 20163, 54505 Vandoeuvre-lès-Nancy, FranceLaboratoire d’Ingénierie des Biomolécules (LIBio), Université de Lorraine, 2 Avenue de la Forêt de Haye–BP 20163, 54505 Vandoeuvre-lès-Nancy, FranceGiven the importance of the extracellular medium during tissue formation, it was wise to develop an artificial structure that mimics the extracellular matrix while having improved physico-chemical properties. That is why the choice was focused on gelatin methacryloyl (GelMA), an inexpensive biocompatible hydrogel. Physicochemical and mechanical properties were improved by the incorporation of nanoparticles developed from two innovative fabrication processes: High shear fluid and low frequencies/high frequencies ultrasounds. Both rapeseed nanoliposomes and nanodroplets were successfully incorporated in the GelMA networks during the photo polymerization process. The impact on polymer microstructure was investigated by Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and enzymatic degradation investigations. Mechanical stability and viscoelastic tests were conducted to demonstrate the beneficial effect of the functionalization on GelMA hydrogels. Adding nanoparticles to GelMA improved the surface properties (porosity), tuned swelling, and degradability properties. In addition, we observed that nanoemulsion didn’t change significantly the mechanical properties to shear and compression solicitations, whereas nanoliposome addition decreased Young’s modulus under compression solicitations. Thus, these ways of functionalization allow controlling the design of the material by choosing the type of nanoparticle (nanoliposome or nanoemulsion) in function of the application.https://www.mdpi.com/1422-0067/18/12/2675GelMAfunctionalized hydrogelnanoliposomenanoemulsionLF/HF ultrasoundsmechanical propertiestissue engineering |
| spellingShingle | Kamel Rahali Ghazi Ben Messaoud Cyril J.F. Kahn Laura Sanchez-Gonzalez Mouna Kaci Franck Cleymand Solenne Fleutot Michel Linder Stéphane Desobry Elmira Arab-Tehrany Synthesis and Characterization of Nanofunctionalized Gelatin Methacrylate Hydrogels International Journal of Molecular Sciences GelMA functionalized hydrogel nanoliposome nanoemulsion LF/HF ultrasounds mechanical properties tissue engineering |
| title | Synthesis and Characterization of Nanofunctionalized Gelatin Methacrylate Hydrogels |
| title_full | Synthesis and Characterization of Nanofunctionalized Gelatin Methacrylate Hydrogels |
| title_fullStr | Synthesis and Characterization of Nanofunctionalized Gelatin Methacrylate Hydrogels |
| title_full_unstemmed | Synthesis and Characterization of Nanofunctionalized Gelatin Methacrylate Hydrogels |
| title_short | Synthesis and Characterization of Nanofunctionalized Gelatin Methacrylate Hydrogels |
| title_sort | synthesis and characterization of nanofunctionalized gelatin methacrylate hydrogels |
| topic | GelMA functionalized hydrogel nanoliposome nanoemulsion LF/HF ultrasounds mechanical properties tissue engineering |
| url | https://www.mdpi.com/1422-0067/18/12/2675 |
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