Comparison of Quantitative Relationship between Real-Time PCR and Acid Fast Bacilli Staining for Diagnosis of Pulmonary Tuberculosis
This study investigates the association of the AFB stain with the cycle threshold (Ct) value of the Cobas TaqMan MTB test (CTM test, Roche Diagnostics, Basel, Switzerland), and it establishes the base data for semi-quantitative identification of M. tuberculosis by the Ct value. CTM test were simulta...
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| Format: | Article |
| Language: | English |
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The Korean Society for Clinical Laboratory Science
2020-12-01
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| Series: | Korean Journal of Clinical Laboratory Science |
| Subjects: |
| _version_ | 1818364075832246272 |
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| author | Taewon Jung Sang-Ha Kim Sunghyun Kim Jae-Sun Choi Young-Kwon Kim |
| author_facet | Taewon Jung Sang-Ha Kim Sunghyun Kim Jae-Sun Choi Young-Kwon Kim |
| author_sort | Taewon Jung |
| collection | DOAJ |
| description | This study investigates the association of the AFB stain with the cycle threshold (Ct) value of the Cobas TaqMan MTB test (CTM test, Roche Diagnostics, Basel, Switzerland), and it establishes the base data for semi-quantitative identification of M. tuberculosis by the Ct value. CTM test were simultaneously conducted on 8,389 specimens submitted to the Samsung Medical Center from January 2015 to December 2015, and the results were analyzed and compared retrospectively investigates the association of the AFB stain with the Ct value of the CTM test, and it establishes the base data for semi-quantitative identification of M. tuberculosis by the Ct value. The Ct values for 135 positive specimens of the CTM were inversely correlated with the AFB stain (rs=−0.545, P＜0.01). When the Ct value of the CTM test and the time to positivity (TTP) of the mycobacteria cultures were verified based on the AFB stain, they were found to have a positive correlation (rs=0.136, P＜0.01). The negative correlation between the CTM test and the AFB stain grade was demonstrated. The clinical significance was verified by applying these criteria to the clinical results. The semi- quantitative criteria of this study can be used to facilitate the rapid isolation of patients with active tuberculosis and infection control in the hospital. |
| first_indexed | 2024-12-13T21:58:36Z |
| format | Article |
| id | doaj.art-5e04a07ba17c424caf2a324208ead8d6 |
| institution | Directory Open Access Journal |
| issn | 1738-3544 2288-1662 |
| language | English |
| last_indexed | 2024-12-13T21:58:36Z |
| publishDate | 2020-12-01 |
| publisher | The Korean Society for Clinical Laboratory Science |
| record_format | Article |
| series | Korean Journal of Clinical Laboratory Science |
| spelling | doaj.art-5e04a07ba17c424caf2a324208ead8d62022-12-21T23:30:05ZengThe Korean Society for Clinical Laboratory ScienceKorean Journal of Clinical Laboratory Science1738-35442288-16622020-12-0152433534110.15324/kjcls.2020.52.4.335Comparison of Quantitative Relationship between Real-Time PCR and Acid Fast Bacilli Staining for Diagnosis of Pulmonary TuberculosisTaewon Jung0Sang-Ha Kim1Sunghyun Kim2Jae-Sun Choi3Young-Kwon Kim4https://orcid.org/0000-0002-5029-4962Department of Laboratory Medicine, Samsung Medical Center, Seoul, KoreaDepartment of Laboratory Medicine, Konyang University Hospital, Daejeon, KoreaDepartment of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan, KoreaDepartment of Biomedical Laboratory Science, Far East University, Eumseong, KoreaDepartment of Health Sciences, The Graduate School of Konyang University, Daejeon, Korea This study investigates the association of the AFB stain with the cycle threshold (Ct) value of the Cobas TaqMan MTB test (CTM test, Roche Diagnostics, Basel, Switzerland), and it establishes the base data for semi-quantitative identification of M. tuberculosis by the Ct value. CTM test were simultaneously conducted on 8,389 specimens submitted to the Samsung Medical Center from January 2015 to December 2015, and the results were analyzed and compared retrospectively investigates the association of the AFB stain with the Ct value of the CTM test, and it establishes the base data for semi-quantitative identification of M. tuberculosis by the Ct value. The Ct values for 135 positive specimens of the CTM were inversely correlated with the AFB stain (rs=−0.545, P＜0.01). When the Ct value of the CTM test and the time to positivity (TTP) of the mycobacteria cultures were verified based on the AFB stain, they were found to have a positive correlation (rs=0.136, P＜0.01). The negative correlation between the CTM test and the AFB stain grade was demonstrated. The clinical significance was verified by applying these criteria to the clinical results. The semi- quantitative criteria of this study can be used to facilitate the rapid isolation of patients with active tuberculosis and infection control in the hospital.cycle threshold valuemycobacterium tuberculosisreal-time pcrsmear positivity grade |
| spellingShingle | Taewon Jung Sang-Ha Kim Sunghyun Kim Jae-Sun Choi Young-Kwon Kim Comparison of Quantitative Relationship between Real-Time PCR and Acid Fast Bacilli Staining for Diagnosis of Pulmonary Tuberculosis Korean Journal of Clinical Laboratory Science cycle threshold value mycobacterium tuberculosis real-time pcr smear positivity grade |
| title | Comparison of Quantitative Relationship between Real-Time PCR and Acid Fast Bacilli Staining for Diagnosis of Pulmonary Tuberculosis |
| title_full | Comparison of Quantitative Relationship between Real-Time PCR and Acid Fast Bacilli Staining for Diagnosis of Pulmonary Tuberculosis |
| title_fullStr | Comparison of Quantitative Relationship between Real-Time PCR and Acid Fast Bacilli Staining for Diagnosis of Pulmonary Tuberculosis |
| title_full_unstemmed | Comparison of Quantitative Relationship between Real-Time PCR and Acid Fast Bacilli Staining for Diagnosis of Pulmonary Tuberculosis |
| title_short | Comparison of Quantitative Relationship between Real-Time PCR and Acid Fast Bacilli Staining for Diagnosis of Pulmonary Tuberculosis |
| title_sort | comparison of quantitative relationship between real time pcr and acid fast bacilli staining for diagnosis of pulmonary tuberculosis |
| topic | cycle threshold value mycobacterium tuberculosis real-time pcr smear positivity grade |
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