Septicaemia of subterranean termites Coptotermes curvignathus caused by disturbance of bacteria isolated from termite gut and its foraging pathways
Microbial pathogens continue to attract a great deal of attention to manage the termite population. Every bacterium has its own mode of action and in fact, the mechanisms used by bacteria to attack termites remain elusive at the moment. Hence, the objective of this study was to evaluate the suscepti...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Royal Society
2020-08-01
|
| Series: | Royal Society Open Science |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.200847 |
| _version_ | 1819171389162127360 |
|---|---|
| author | Kit Ling Chin Paik San H'ng Wan Zhen Wong Chuan Li Lee Pui San Khoo Abdullah Chuah Luqman Zaidon Ashaari Seca Gandaseca |
| author_facet | Kit Ling Chin Paik San H'ng Wan Zhen Wong Chuan Li Lee Pui San Khoo Abdullah Chuah Luqman Zaidon Ashaari Seca Gandaseca |
| author_sort | Kit Ling Chin |
| collection | DOAJ |
| description | Microbial pathogens continue to attract a great deal of attention to manage the termite population. Every bacterium has its own mode of action and in fact, the mechanisms used by bacteria to attack termites remain elusive at the moment. Hence, the objective of this study was to evaluate the susceptibility of subterranean termites Coptotermes curvignathus to opportunistic pathogens using culturable aerobic bacteria isolated from the termite gut and its foraging pathways. Bacterial suspensions were prepared in concentrations of 103, 106 and 109 colony-forming units (CFU) ml−1 and introduced to the termites via oral-contact and physical contact treatment. The data show that contact method acted slower and gave lower mortality, compared to the oral-contact method. Coptotermes curvignathus were highly susceptible to Serratia marcescens and Pseudomonas aeruginosa. Serratia marcescens showed the highest mortality percentage of 68% and 54% at bacterial concentration of 109 CFU ml−1 via oral-contact and contact method, respectively. Serratia marcescens was also defined as the bacteria with the highest ability to induce the high mortality of C. curvignathus with the lowest concentration of bacterial suspension at a given time under laboratory condition. The results of this study indicate that P. aeruginosa and S. marcescens in particular may be attractive candidates worth further examination as a possible biocontrol agent against C. curvignathus in the field and to evaluate environmental and ecological risks of the biocontrol. |
| first_indexed | 2024-12-22T19:50:30Z |
| format | Article |
| id | doaj.art-5e056edeaf7246c4a43f449bcc770f0b |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-12-22T19:50:30Z |
| publishDate | 2020-08-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-5e056edeaf7246c4a43f449bcc770f0b2022-12-21T18:14:35ZengThe Royal SocietyRoyal Society Open Science2054-57032020-08-017810.1098/rsos.200847200847Septicaemia of subterranean termites Coptotermes curvignathus caused by disturbance of bacteria isolated from termite gut and its foraging pathwaysKit Ling ChinPaik San H'ngWan Zhen WongChuan Li LeePui San KhooAbdullah Chuah LuqmanZaidon AshaariSeca GandasecaMicrobial pathogens continue to attract a great deal of attention to manage the termite population. Every bacterium has its own mode of action and in fact, the mechanisms used by bacteria to attack termites remain elusive at the moment. Hence, the objective of this study was to evaluate the susceptibility of subterranean termites Coptotermes curvignathus to opportunistic pathogens using culturable aerobic bacteria isolated from the termite gut and its foraging pathways. Bacterial suspensions were prepared in concentrations of 103, 106 and 109 colony-forming units (CFU) ml−1 and introduced to the termites via oral-contact and physical contact treatment. The data show that contact method acted slower and gave lower mortality, compared to the oral-contact method. Coptotermes curvignathus were highly susceptible to Serratia marcescens and Pseudomonas aeruginosa. Serratia marcescens showed the highest mortality percentage of 68% and 54% at bacterial concentration of 109 CFU ml−1 via oral-contact and contact method, respectively. Serratia marcescens was also defined as the bacteria with the highest ability to induce the high mortality of C. curvignathus with the lowest concentration of bacterial suspension at a given time under laboratory condition. The results of this study indicate that P. aeruginosa and S. marcescens in particular may be attractive candidates worth further examination as a possible biocontrol agent against C. curvignathus in the field and to evaluate environmental and ecological risks of the biocontrol.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.200847coptotermes curvignathusby oral-contactby contactbacterial concentrationbiocontrol efficiency |
| spellingShingle | Kit Ling Chin Paik San H'ng Wan Zhen Wong Chuan Li Lee Pui San Khoo Abdullah Chuah Luqman Zaidon Ashaari Seca Gandaseca Septicaemia of subterranean termites Coptotermes curvignathus caused by disturbance of bacteria isolated from termite gut and its foraging pathways Royal Society Open Science coptotermes curvignathus by oral-contact by contact bacterial concentration biocontrol efficiency |
| title | Septicaemia of subterranean termites Coptotermes curvignathus caused by disturbance of bacteria isolated from termite gut and its foraging pathways |
| title_full | Septicaemia of subterranean termites Coptotermes curvignathus caused by disturbance of bacteria isolated from termite gut and its foraging pathways |
| title_fullStr | Septicaemia of subterranean termites Coptotermes curvignathus caused by disturbance of bacteria isolated from termite gut and its foraging pathways |
| title_full_unstemmed | Septicaemia of subterranean termites Coptotermes curvignathus caused by disturbance of bacteria isolated from termite gut and its foraging pathways |
| title_short | Septicaemia of subterranean termites Coptotermes curvignathus caused by disturbance of bacteria isolated from termite gut and its foraging pathways |
| title_sort | septicaemia of subterranean termites coptotermes curvignathus caused by disturbance of bacteria isolated from termite gut and its foraging pathways |
| topic | coptotermes curvignathus by oral-contact by contact bacterial concentration biocontrol efficiency |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.200847 |
| work_keys_str_mv | AT kitlingchin septicaemiaofsubterraneantermitescoptotermescurvignathuscausedbydisturbanceofbacteriaisolatedfromtermitegutanditsforagingpathways AT paiksanhng septicaemiaofsubterraneantermitescoptotermescurvignathuscausedbydisturbanceofbacteriaisolatedfromtermitegutanditsforagingpathways AT wanzhenwong septicaemiaofsubterraneantermitescoptotermescurvignathuscausedbydisturbanceofbacteriaisolatedfromtermitegutanditsforagingpathways AT chuanlilee septicaemiaofsubterraneantermitescoptotermescurvignathuscausedbydisturbanceofbacteriaisolatedfromtermitegutanditsforagingpathways AT puisankhoo septicaemiaofsubterraneantermitescoptotermescurvignathuscausedbydisturbanceofbacteriaisolatedfromtermitegutanditsforagingpathways AT abdullahchuahluqman septicaemiaofsubterraneantermitescoptotermescurvignathuscausedbydisturbanceofbacteriaisolatedfromtermitegutanditsforagingpathways AT zaidonashaari septicaemiaofsubterraneantermitescoptotermescurvignathuscausedbydisturbanceofbacteriaisolatedfromtermitegutanditsforagingpathways AT secagandaseca septicaemiaofsubterraneantermitescoptotermescurvignathuscausedbydisturbanceofbacteriaisolatedfromtermitegutanditsforagingpathways |