Biochemical and histopathological responses of Biomphalaria alexandrina to RIPEX (plant growth regulator)
Abstract Background Plant growth regulators are widely used in agriculture for increasing the growth and ripening of plants, but they endanger the aquatic ecosystem. The current study assessed the effect of sublethal exposure to RIPEX 48% EC concentrations (8 and 16 µL/L) on oxidative stress paramet...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2023-04-01
|
| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43088-023-00378-5 |
| _version_ | 1797840852811251712 |
|---|---|
| author | Hoda H. Abdel-Azeem Azza H. Mohamed Mohamed R. Habib |
| author_facet | Hoda H. Abdel-Azeem Azza H. Mohamed Mohamed R. Habib |
| author_sort | Hoda H. Abdel-Azeem |
| collection | DOAJ |
| description | Abstract Background Plant growth regulators are widely used in agriculture for increasing the growth and ripening of plants, but they endanger the aquatic ecosystem. The current study assessed the effect of sublethal exposure to RIPEX 48% EC concentrations (8 and 16 µL/L) on oxidative stress parameters, sex hormones, immune potential enzymes, differential hemocyte counts, and the histopathology of digestive glands and ovotestis in Biomphalaria alexandrina snails. Results RIPEX exposure caused an overall increase in Superoxide dismutase and Glutathione-S-Transferase activities in B. alexandrina. However, extreme RIPEX exposure inhibits SOD activity in snails. Malondialdehyde activity showed an increase in B. alexandrina exposed to both concentrations after all exposure periods. RIPEX also caused a significant increase in testosterone in snails exposed to 16 µL/L, it did, however, reduce hormone levels in snails exposed to 8 µL/L at 7 days. Regarding estradiol, there was a significant increase after 3 days of exposure to 16 µl/L and 7 days of exposure to 8 µL/L. RIPEX exposure also increased the activities of Myeloperoxidase and Adenosine deaminase enzymes in the digestive glands of snails. It increased the total hemocyte count of exposed snails as well as the number of granulocytes. Snail digestive glands and ovotestis showed pathological alterations after 7 days of RIPEX exposure. Conclusions These findings suggest that RIPEX is toxic to B. alexandrina and that this snail can be used as a bioindicator for environmental contamination with plant growth regulators. |
| first_indexed | 2024-04-09T16:21:30Z |
| format | Article |
| id | doaj.art-5e05ae583b054e9f88fe938edbc58b74 |
| institution | Directory Open Access Journal |
| issn | 2314-8543 |
| language | English |
| last_indexed | 2024-04-09T16:21:30Z |
| publishDate | 2023-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-5e05ae583b054e9f88fe938edbc58b742023-04-23T11:25:22ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85432023-04-0112111110.1186/s43088-023-00378-5Biochemical and histopathological responses of Biomphalaria alexandrina to RIPEX (plant growth regulator)Hoda H. Abdel-Azeem0Azza H. Mohamed1Mohamed R. Habib2Department of Zoology, Faculty of Science, Menoufia UniversityDepartment of Zoology, Faculty of Science, Menoufia UniversityMedical Malacology Department, Theodor Bilharz Research InstituteAbstract Background Plant growth regulators are widely used in agriculture for increasing the growth and ripening of plants, but they endanger the aquatic ecosystem. The current study assessed the effect of sublethal exposure to RIPEX 48% EC concentrations (8 and 16 µL/L) on oxidative stress parameters, sex hormones, immune potential enzymes, differential hemocyte counts, and the histopathology of digestive glands and ovotestis in Biomphalaria alexandrina snails. Results RIPEX exposure caused an overall increase in Superoxide dismutase and Glutathione-S-Transferase activities in B. alexandrina. However, extreme RIPEX exposure inhibits SOD activity in snails. Malondialdehyde activity showed an increase in B. alexandrina exposed to both concentrations after all exposure periods. RIPEX also caused a significant increase in testosterone in snails exposed to 16 µL/L, it did, however, reduce hormone levels in snails exposed to 8 µL/L at 7 days. Regarding estradiol, there was a significant increase after 3 days of exposure to 16 µl/L and 7 days of exposure to 8 µL/L. RIPEX exposure also increased the activities of Myeloperoxidase and Adenosine deaminase enzymes in the digestive glands of snails. It increased the total hemocyte count of exposed snails as well as the number of granulocytes. Snail digestive glands and ovotestis showed pathological alterations after 7 days of RIPEX exposure. Conclusions These findings suggest that RIPEX is toxic to B. alexandrina and that this snail can be used as a bioindicator for environmental contamination with plant growth regulators.https://doi.org/10.1186/s43088-023-00378-5Biomphalaria alexandrinaRIPEXTestosteroneEstradiolMyeloperoxidaseAdenosine deaminase |
| spellingShingle | Hoda H. Abdel-Azeem Azza H. Mohamed Mohamed R. Habib Biochemical and histopathological responses of Biomphalaria alexandrina to RIPEX (plant growth regulator) Beni-Suef University Journal of Basic and Applied Sciences Biomphalaria alexandrina RIPEX Testosterone Estradiol Myeloperoxidase Adenosine deaminase |
| title | Biochemical and histopathological responses of Biomphalaria alexandrina to RIPEX (plant growth regulator) |
| title_full | Biochemical and histopathological responses of Biomphalaria alexandrina to RIPEX (plant growth regulator) |
| title_fullStr | Biochemical and histopathological responses of Biomphalaria alexandrina to RIPEX (plant growth regulator) |
| title_full_unstemmed | Biochemical and histopathological responses of Biomphalaria alexandrina to RIPEX (plant growth regulator) |
| title_short | Biochemical and histopathological responses of Biomphalaria alexandrina to RIPEX (plant growth regulator) |
| title_sort | biochemical and histopathological responses of biomphalaria alexandrina to ripex plant growth regulator |
| topic | Biomphalaria alexandrina RIPEX Testosterone Estradiol Myeloperoxidase Adenosine deaminase |
| url | https://doi.org/10.1186/s43088-023-00378-5 |
| work_keys_str_mv | AT hodahabdelazeem biochemicalandhistopathologicalresponsesofbiomphalariaalexandrinatoripexplantgrowthregulator AT azzahmohamed biochemicalandhistopathologicalresponsesofbiomphalariaalexandrinatoripexplantgrowthregulator AT mohamedrhabib biochemicalandhistopathologicalresponsesofbiomphalariaalexandrinatoripexplantgrowthregulator |