Induction of defence enzymes activities in grape plant treated by seaweed algae against Plasmopara viticola and Uncinula necator causing downy and powdery mildews of grapes
Plasmopara viticola and Uncinula necator are the causative agents of downy mildew and powdery mildew diseases, respectively, which become a major problem in grape crop (Vitis vinifera L.). The use of natural products such as seaweed provides a rich source of structurally diverse and biologically a...
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The National Information and Documentation Centre (NIDOC) , Egypt
2018-12-01
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| Series: | Novel Research in Microbiology Journal |
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| Online Access: | https://nrmj.journals.ekb.eg/article_22862_bec5296f1220c2b83fea93965c2abe24.pdf |
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| author | T. Suthin Raj S. Vignesh P. Nishanthi K. Hane graff H. Ann Suji |
| author_facet | T. Suthin Raj S. Vignesh P. Nishanthi K. Hane graff H. Ann Suji |
| author_sort | T. Suthin Raj |
| collection | DOAJ |
| description | Plasmopara viticola and Uncinula necator are the causative agents of downy mildew and powdery mildew
diseases, respectively, which become a major problem in grape crop (Vitis vinifera L.). The use of natural products
such as seaweed provides a rich source of structurally diverse and biologically active secondary metabolites; and is
the ultimate way of combating these diseases. In this context, six different seaweed algae such as; Sargassum
wightii, Padina gymospora, Caulerpa peltata, Halimeda gracilis, Acanthophora spicifera and Gracilaria opuntia;
were used to control the downy and powdery mildew diseases of grapes. Enzymatic studies were carried out during
two growing seasons 1 and 2 on peroxidase; polyphenol oxidase, phenylalanine ammonia lyase and β-1,3-
glucanase. During season 1; in grape plants treated with Sargassum wightii, the four enzymes raised the glucose
concentration to 60.79µg, 3.87µg, 5.20µg and 225.5µg; respectively, and then these plants were treated with
Bordeaux mixture at 1%. After seaweed application; enzymes raised the glucose level to 59.83µg, 3.57µg, 5.17µg,
221.7µg. During season 2; when plants were treated with Sargassum wightii, the enzymes raised the glucose
concentration to 62.48µg, 5.93µg, 7.25µg and 226.2µg, and then these plants were treated with Bordeaux mixture at
1%. After application; the enzymes raised the glucose level to 61.92µg, 3.57µg, 7.21µg and 224.2µg, respectively.
During both seasons; β-1,3-glucanase released more amounts of glucose in both normal plants and those treated
with Bordeaux mixture. The present study was undertaken to evaluate whether various extracts of seaweeds algae
such as; brown, red, and green seaweeds along with the defence enzymes studied, would increase the resistance of
grapes to downy and powdery mildews. |
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| issn | 2537-0286 2537-0294 |
| language | English |
| last_indexed | 2024-04-24T08:30:03Z |
| publishDate | 2018-12-01 |
| publisher | The National Information and Documentation Centre (NIDOC) , Egypt |
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| series | Novel Research in Microbiology Journal |
| spelling | doaj.art-5e078ac8ec644b45b60a94bdfab922f52024-04-16T20:24:39ZengThe National Information and Documentation Centre (NIDOC) , EgyptNovel Research in Microbiology Journal2537-02862537-02942018-12-0126122137Induction of defence enzymes activities in grape plant treated by seaweed algae against Plasmopara viticola and Uncinula necator causing downy and powdery mildews of grapesT. Suthin Raj0S. Vignesh1P. Nishanthi 2K. Hane graff3 H. Ann Suji4Department of Plant Pathology, Faculty of Agriculture, Annamalai University, Chidambaram, IndiaDepartment of Plant Pathology, Faculty of Agriculture, Annamalai University, Chidambaram, IndiaDepartment of Plant Pathology, Faculty of Agriculture, Annamalai University, Chidambaram, IndiaDepartment of Plant Pathology, Faculty of Agriculture, Annamalai University, Chidambaram, IndiaCentre for Advance Studies in Marine Biology, Annamalai University, Chidambaram (T.N.), IndiaPlasmopara viticola and Uncinula necator are the causative agents of downy mildew and powdery mildew diseases, respectively, which become a major problem in grape crop (Vitis vinifera L.). The use of natural products such as seaweed provides a rich source of structurally diverse and biologically active secondary metabolites; and is the ultimate way of combating these diseases. In this context, six different seaweed algae such as; Sargassum wightii, Padina gymospora, Caulerpa peltata, Halimeda gracilis, Acanthophora spicifera and Gracilaria opuntia; were used to control the downy and powdery mildew diseases of grapes. Enzymatic studies were carried out during two growing seasons 1 and 2 on peroxidase; polyphenol oxidase, phenylalanine ammonia lyase and β-1,3- glucanase. During season 1; in grape plants treated with Sargassum wightii, the four enzymes raised the glucose concentration to 60.79µg, 3.87µg, 5.20µg and 225.5µg; respectively, and then these plants were treated with Bordeaux mixture at 1%. After seaweed application; enzymes raised the glucose level to 59.83µg, 3.57µg, 5.17µg, 221.7µg. During season 2; when plants were treated with Sargassum wightii, the enzymes raised the glucose concentration to 62.48µg, 5.93µg, 7.25µg and 226.2µg, and then these plants were treated with Bordeaux mixture at 1%. After application; the enzymes raised the glucose level to 61.92µg, 3.57µg, 7.21µg and 224.2µg, respectively. During both seasons; β-1,3-glucanase released more amounts of glucose in both normal plants and those treated with Bordeaux mixture. The present study was undertaken to evaluate whether various extracts of seaweeds algae such as; brown, red, and green seaweeds along with the defence enzymes studied, would increase the resistance of grapes to downy and powdery mildews.https://nrmj.journals.ekb.eg/article_22862_bec5296f1220c2b83fea93965c2abe24.pdfgrapesseaweeds algaeperoxidasepolyphenol oxidasephenylalanine ammonia lyaseglucanase |
| spellingShingle | T. Suthin Raj S. Vignesh P. Nishanthi K. Hane graff H. Ann Suji Induction of defence enzymes activities in grape plant treated by seaweed algae against Plasmopara viticola and Uncinula necator causing downy and powdery mildews of grapes Novel Research in Microbiology Journal grapes seaweeds algae peroxidase polyphenol oxidase phenylalanine ammonia lyase glucanase |
| title | Induction of defence enzymes activities in grape plant treated by seaweed algae against Plasmopara viticola and Uncinula necator causing downy and powdery mildews of grapes |
| title_full | Induction of defence enzymes activities in grape plant treated by seaweed algae against Plasmopara viticola and Uncinula necator causing downy and powdery mildews of grapes |
| title_fullStr | Induction of defence enzymes activities in grape plant treated by seaweed algae against Plasmopara viticola and Uncinula necator causing downy and powdery mildews of grapes |
| title_full_unstemmed | Induction of defence enzymes activities in grape plant treated by seaweed algae against Plasmopara viticola and Uncinula necator causing downy and powdery mildews of grapes |
| title_short | Induction of defence enzymes activities in grape plant treated by seaweed algae against Plasmopara viticola and Uncinula necator causing downy and powdery mildews of grapes |
| title_sort | induction of defence enzymes activities in grape plant treated by seaweed algae against plasmopara viticola and uncinula necator causing downy and powdery mildews of grapes |
| topic | grapes seaweeds algae peroxidase polyphenol oxidase phenylalanine ammonia lyase glucanase |
| url | https://nrmj.journals.ekb.eg/article_22862_bec5296f1220c2b83fea93965c2abe24.pdf |
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