Transcriptome and metabolome analysis reveals anthocyanin biosynthesis pathway associated with ramie (Boehmeria nivea (L.) Gaud.) leaf color formation
Abstract Background The bast fiber crop ramie can be used as high-quality forage resources, especially in tropical or subtropical region where there is lack of high-quality protein feed. Hongxuan No.1 (HX_1) is a unique ramie variety with a light reddish brown leaf color, which is obviously differen...
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| Format: | Article |
| Language: | English |
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BMC
2021-09-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-021-08007-0 |
| _version_ | 1818652435482148864 |
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| author | Xinkang Feng Gang Gao Chunming Yu Aiguo Zhu Jikang Chen Kunmei Chen Xiaofei Wang Aminu Shehu Abubakar Ping Chen |
| author_facet | Xinkang Feng Gang Gao Chunming Yu Aiguo Zhu Jikang Chen Kunmei Chen Xiaofei Wang Aminu Shehu Abubakar Ping Chen |
| author_sort | Xinkang Feng |
| collection | DOAJ |
| description | Abstract Background The bast fiber crop ramie can be used as high-quality forage resources, especially in tropical or subtropical region where there is lack of high-quality protein feed. Hongxuan No.1 (HX_1) is a unique ramie variety with a light reddish brown leaf color, which is obviously different from elite cultivar, Zhongzhu No.1 (ZZ_1, green leaf). While, the regulatory mechanism of color difference or secondary metaboliates synthesis between these two varieties have not been studied. Results In this study, phenotypic, transcriptomic and metabolomic analysis of HX_1 and ZZ_1 were conducted to elucidate the mechanism of leaf color formation. Chromaticity value and pigment content measuring showed that anthocyanin was the main metabolites imparting the different leaf color phenotype between the two varieties. Based on LC/MS, at least 14 anthocyanins were identified in leaves of HX_1 and ZZ_1, and the HX_1 showed the higher relative content of malvidin-, pelargonidin-,and cyanidin-based anthocyanins. Transcriptome and metabolome co-analysis revealed that the up-regulated expression of flavonoids synthesis gene was positively correlated with total anthocyanins accumulation in ramie leaf, and the differentfially expression of “blue gene” (F3’5’H) and the “red gene” (F3’H) in leaves bring out HX_1 metabolic flow more input into the cyanidin branch. Furthermore, the enrichment of glycosylated modification pathway (UGT and AT) and the expression of flavonoid 3-O-glucosyl transferase (UFGT), anthocyanidin reductase (ANR), in leaves were significantly influenced the diversity of anthocyanins between HX_1 and ZZ_1. Conclusions Phenotypic, transcriptomic and metabolomic analysis of HX_1 and ZZ_1 indicated that the expression levels of genes related to anthocyanin metabolism contribute to the color formation of ramie variety. Anthocyanins are important plant secandary metabilates with many physiological functions, the results of this study will deepened our understanding of ramie leaf color formation, and provided basis for molecular breeding of functional forage ramie. |
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| format | Article |
| id | doaj.art-5e07f8e9efff4644a71c9283eb3c0f4e |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-17T02:21:58Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
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| series | BMC Genomics |
| spelling | doaj.art-5e07f8e9efff4644a71c9283eb3c0f4e2022-12-21T22:07:15ZengBMCBMC Genomics1471-21642021-09-0122111510.1186/s12864-021-08007-0Transcriptome and metabolome analysis reveals anthocyanin biosynthesis pathway associated with ramie (Boehmeria nivea (L.) Gaud.) leaf color formationXinkang Feng0Gang Gao1Chunming Yu2Aiguo Zhu3Jikang Chen4Kunmei Chen5Xiaofei Wang6Aminu Shehu Abubakar7Ping Chen8Institute of Bast Fiber Crops, Chinese Academy of Agricultural SciencesInstitute of Bast Fiber Crops, Chinese Academy of Agricultural SciencesInstitute of Bast Fiber Crops, Chinese Academy of Agricultural SciencesInstitute of Bast Fiber Crops, Chinese Academy of Agricultural SciencesInstitute of Bast Fiber Crops, Chinese Academy of Agricultural SciencesInstitute of Bast Fiber Crops, Chinese Academy of Agricultural SciencesInstitute of Bast Fiber Crops, Chinese Academy of Agricultural SciencesInstitute of Bast Fiber Crops, Chinese Academy of Agricultural SciencesInstitute of Bast Fiber Crops, Chinese Academy of Agricultural SciencesAbstract Background The bast fiber crop ramie can be used as high-quality forage resources, especially in tropical or subtropical region where there is lack of high-quality protein feed. Hongxuan No.1 (HX_1) is a unique ramie variety with a light reddish brown leaf color, which is obviously different from elite cultivar, Zhongzhu No.1 (ZZ_1, green leaf). While, the regulatory mechanism of color difference or secondary metaboliates synthesis between these two varieties have not been studied. Results In this study, phenotypic, transcriptomic and metabolomic analysis of HX_1 and ZZ_1 were conducted to elucidate the mechanism of leaf color formation. Chromaticity value and pigment content measuring showed that anthocyanin was the main metabolites imparting the different leaf color phenotype between the two varieties. Based on LC/MS, at least 14 anthocyanins were identified in leaves of HX_1 and ZZ_1, and the HX_1 showed the higher relative content of malvidin-, pelargonidin-,and cyanidin-based anthocyanins. Transcriptome and metabolome co-analysis revealed that the up-regulated expression of flavonoids synthesis gene was positively correlated with total anthocyanins accumulation in ramie leaf, and the differentfially expression of “blue gene” (F3’5’H) and the “red gene” (F3’H) in leaves bring out HX_1 metabolic flow more input into the cyanidin branch. Furthermore, the enrichment of glycosylated modification pathway (UGT and AT) and the expression of flavonoid 3-O-glucosyl transferase (UFGT), anthocyanidin reductase (ANR), in leaves were significantly influenced the diversity of anthocyanins between HX_1 and ZZ_1. Conclusions Phenotypic, transcriptomic and metabolomic analysis of HX_1 and ZZ_1 indicated that the expression levels of genes related to anthocyanin metabolism contribute to the color formation of ramie variety. Anthocyanins are important plant secandary metabilates with many physiological functions, the results of this study will deepened our understanding of ramie leaf color formation, and provided basis for molecular breeding of functional forage ramie.https://doi.org/10.1186/s12864-021-08007-0Ramie varietyLeafColor formationMetabolitesAnthocyanins |
| spellingShingle | Xinkang Feng Gang Gao Chunming Yu Aiguo Zhu Jikang Chen Kunmei Chen Xiaofei Wang Aminu Shehu Abubakar Ping Chen Transcriptome and metabolome analysis reveals anthocyanin biosynthesis pathway associated with ramie (Boehmeria nivea (L.) Gaud.) leaf color formation BMC Genomics Ramie variety Leaf Color formation Metabolites Anthocyanins |
| title | Transcriptome and metabolome analysis reveals anthocyanin biosynthesis pathway associated with ramie (Boehmeria nivea (L.) Gaud.) leaf color formation |
| title_full | Transcriptome and metabolome analysis reveals anthocyanin biosynthesis pathway associated with ramie (Boehmeria nivea (L.) Gaud.) leaf color formation |
| title_fullStr | Transcriptome and metabolome analysis reveals anthocyanin biosynthesis pathway associated with ramie (Boehmeria nivea (L.) Gaud.) leaf color formation |
| title_full_unstemmed | Transcriptome and metabolome analysis reveals anthocyanin biosynthesis pathway associated with ramie (Boehmeria nivea (L.) Gaud.) leaf color formation |
| title_short | Transcriptome and metabolome analysis reveals anthocyanin biosynthesis pathway associated with ramie (Boehmeria nivea (L.) Gaud.) leaf color formation |
| title_sort | transcriptome and metabolome analysis reveals anthocyanin biosynthesis pathway associated with ramie boehmeria nivea l gaud leaf color formation |
| topic | Ramie variety Leaf Color formation Metabolites Anthocyanins |
| url | https://doi.org/10.1186/s12864-021-08007-0 |
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