Immune reconstitution following umbilical cord blood transplantation: IRES, a study of UK paediatric patients
Abstract To obtain a qualitative as well as quantitative view immune reconstitution following umbilical cord blood (UCB) transplantation of paediatric patients, we utilised a broad panel of flow cytometry markers to monitor the phenotypes of lymphoid and myeloid cells at 1‐12 months post‐transplant....
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-07-01
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| Series: | eJHaem |
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| Online Access: | https://doi.org/10.1002/jha2.12 |
| _version_ | 1827863146208952320 |
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| author | John Girdlestone Meera Raymond Bronwen Shaw Sameer Tulpule Vikesh R. Devlia Robert Danby Trudy Ahyee Aurore Saudemont Rachael Hough Paul Veys Annalisa Ruggeri Ajay Vora David I. Marks Brenda Gibson Robert Wynn Alejandro Madrigal Cristina V. Navarrete |
| author_facet | John Girdlestone Meera Raymond Bronwen Shaw Sameer Tulpule Vikesh R. Devlia Robert Danby Trudy Ahyee Aurore Saudemont Rachael Hough Paul Veys Annalisa Ruggeri Ajay Vora David I. Marks Brenda Gibson Robert Wynn Alejandro Madrigal Cristina V. Navarrete |
| author_sort | John Girdlestone |
| collection | DOAJ |
| description | Abstract To obtain a qualitative as well as quantitative view immune reconstitution following umbilical cord blood (UCB) transplantation of paediatric patients, we utilised a broad panel of flow cytometry markers to monitor the phenotypes of lymphoid and myeloid cells at 1‐12 months post‐transplant. Samples were received from 46 patients with a median age of 3.3 years and survival was 76% at 1 year. Monocytes were at similar or higher median levels than in adult controls at all times tested, with a high CD16+ proportion in the first 3 months. NK cells were also within adult ranges, with a CD56++ high proportion in the first 6 months. B cell recovery was seen from 2 months in most patients and T cells from 3 months, both were delayed with anti‐thymocyte globulin (ATG) treatment. CD4:CD8 ratios were high in the first 6 months, and the proportion of T cells with recent thymic emigrant and naïve phenotypes rose from 3 months. NK and plasmacytoid dendritic cell numbers remained at reduced levels in patients not surviving to 1 year. Our results can serve as a useful reference for detailed monitoring of immune reconstitution in paediatric recipients of UCB. |
| first_indexed | 2024-03-12T14:05:42Z |
| format | Article |
| id | doaj.art-5e08f9b7f453485e971b21e158fc84b1 |
| institution | Directory Open Access Journal |
| issn | 2688-6146 |
| language | English |
| last_indexed | 2024-03-12T14:05:42Z |
| publishDate | 2020-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | eJHaem |
| spelling | doaj.art-5e08f9b7f453485e971b21e158fc84b12023-08-21T14:10:49ZengWileyeJHaem2688-61462020-07-011120821810.1002/jha2.12Immune reconstitution following umbilical cord blood transplantation: IRES, a study of UK paediatric patientsJohn Girdlestone0Meera Raymond1Bronwen Shaw2Sameer Tulpule3Vikesh R. Devlia4Robert Danby5Trudy Ahyee6Aurore Saudemont7Rachael Hough8Paul Veys9Annalisa Ruggeri10Ajay Vora11David I. Marks12Brenda Gibson13Robert Wynn14Alejandro Madrigal15Cristina V. Navarrete16H&I R&D Group NHSBT Colindale Centre London UKH&I R&D Group NHSBT Colindale Centre London UKCenter for International Blood and Marrow Transplant Research Medical College of Wisconsin Milwaukee WisconsinDepartment of Haematology Kokilaben Dhirubhai Ambani Hospital Mumbai IndiaDepartment of Immunotherapy Anthony Nolan Research Institute London UKDepartment of Immunotherapy Anthony Nolan Research Institute London UKDepartment of Immunotherapy Anthony Nolan Research Institute London UKDepartment of Immunotherapy Anthony Nolan Research Institute London UKDepartment of Haematology University College London Hospitals London UKBone Marrow Transplant Unit Great Ormond Street Hospital London UKEurocord Paris FranceBone Marrow Transplant Unit Great Ormond Street Hospital London UKBristol Haematology and Oncology Centre University Hospitals Bristol Bristol UKPaediatric Haematology Royal Hospital for Sick Children Glasgow UKPaediatric Bone Marrow Transplant Programme Royal Manchester Children's Hospital Manchester UKDepartment of Immunotherapy Anthony Nolan Research Institute London UKH&I R&D Group NHSBT Colindale Centre London UKAbstract To obtain a qualitative as well as quantitative view immune reconstitution following umbilical cord blood (UCB) transplantation of paediatric patients, we utilised a broad panel of flow cytometry markers to monitor the phenotypes of lymphoid and myeloid cells at 1‐12 months post‐transplant. Samples were received from 46 patients with a median age of 3.3 years and survival was 76% at 1 year. Monocytes were at similar or higher median levels than in adult controls at all times tested, with a high CD16+ proportion in the first 3 months. NK cells were also within adult ranges, with a CD56++ high proportion in the first 6 months. B cell recovery was seen from 2 months in most patients and T cells from 3 months, both were delayed with anti‐thymocyte globulin (ATG) treatment. CD4:CD8 ratios were high in the first 6 months, and the proportion of T cells with recent thymic emigrant and naïve phenotypes rose from 3 months. NK and plasmacytoid dendritic cell numbers remained at reduced levels in patients not surviving to 1 year. Our results can serve as a useful reference for detailed monitoring of immune reconstitution in paediatric recipients of UCB.https://doi.org/10.1002/jha2.12cord bloodimmunophenotypingstem cell transplantation |
| spellingShingle | John Girdlestone Meera Raymond Bronwen Shaw Sameer Tulpule Vikesh R. Devlia Robert Danby Trudy Ahyee Aurore Saudemont Rachael Hough Paul Veys Annalisa Ruggeri Ajay Vora David I. Marks Brenda Gibson Robert Wynn Alejandro Madrigal Cristina V. Navarrete Immune reconstitution following umbilical cord blood transplantation: IRES, a study of UK paediatric patients eJHaem cord blood immunophenotyping stem cell transplantation |
| title | Immune reconstitution following umbilical cord blood transplantation: IRES, a study of UK paediatric patients |
| title_full | Immune reconstitution following umbilical cord blood transplantation: IRES, a study of UK paediatric patients |
| title_fullStr | Immune reconstitution following umbilical cord blood transplantation: IRES, a study of UK paediatric patients |
| title_full_unstemmed | Immune reconstitution following umbilical cord blood transplantation: IRES, a study of UK paediatric patients |
| title_short | Immune reconstitution following umbilical cord blood transplantation: IRES, a study of UK paediatric patients |
| title_sort | immune reconstitution following umbilical cord blood transplantation ires a study of uk paediatric patients |
| topic | cord blood immunophenotyping stem cell transplantation |
| url | https://doi.org/10.1002/jha2.12 |
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