Lonicerin attenuates house dust mite-induced eosinophilic asthma through targeting Src/EGFR signaling
Eosinophilic asthma is the predominant phenotype of asthma, and although these patients are sensitive to glucocorticoid therapy, they also experience many side effects. Lonicerin is a kind of bioflavonoid isolated from the Chinese herb Lonicera japonica Thunb, which has anti-inflammatory and immunom...
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| Format: | Article |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.1051344/full |
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| author | Zhenan Deng Xuefei Zhang Junjie Wen Xiaojing Yang Lingna Xue Changxing Ou Jianjuan Ma Hongrui Zhan Xiaomin Cen Xuliang Cai Yu Zhang Riken Chen Qingling Zhang |
| author_facet | Zhenan Deng Xuefei Zhang Junjie Wen Xiaojing Yang Lingna Xue Changxing Ou Jianjuan Ma Hongrui Zhan Xiaomin Cen Xuliang Cai Yu Zhang Riken Chen Qingling Zhang |
| author_sort | Zhenan Deng |
| collection | DOAJ |
| description | Eosinophilic asthma is the predominant phenotype of asthma, and although these patients are sensitive to glucocorticoid therapy, they also experience many side effects. Lonicerin is a kind of bioflavonoid isolated from the Chinese herb Lonicera japonica Thunb, which has anti-inflammatory and immunomodulatory effects. The aim of this study was to elucidate the effects of lonicerin on eosinophilic asthma and its potential mechanisms. Here, we established a house dust mite (house dust mite)-induced eosinophilic asthma model in BALB/c mouse, and evaluated the effects of lonicerin on it. Our results showed that lonicerin significantly reduced airway hyperresponsiveness the number of inflammatory cells (especially eosinophils) and the elevation of interleukin (IL)-4, IL-5, IL-13 and eotaxin in bronchoalveolar lavage fluid (BALF) supernatants of mice. Additionally, lonicerin also eminently blunted inflammatory infiltration and mucus secretion, as well as mRNA levels of Mucin 5AC (MUC5AC) in lung tissue. Furthermore, results of network pharmacology and molecular docking revealed that Src kinase and epidermal growth factor receptor may be the potential targets responsible for the effects of lonicerin. Finally, in vivo experiments confirmed that lonicerin inhibited activation of the Src/EGFR pathway by decreasing their phosphorylation. Taken together, the present study demonstrated that lonicerin could suppress HDM-induced eosinophilic asthma in mice through inhibiting the activation of Src/EGFR pathway, which also provides a basis for further research as a new potentially therapeutic agent for eosinophilic asthma and its underlying mechanisms in the future. |
| first_indexed | 2024-04-11T05:24:53Z |
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| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-11T05:24:53Z |
| publishDate | 2022-12-01 |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-5e0b768a77ae48208b5337afb0e6b3522022-12-23T07:45:53ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-12-011310.3389/fphar.2022.10513441051344Lonicerin attenuates house dust mite-induced eosinophilic asthma through targeting Src/EGFR signalingZhenan Deng0Xuefei Zhang1Junjie Wen2Xiaojing Yang3Lingna Xue4Changxing Ou5Jianjuan Ma6Hongrui Zhan7Xiaomin Cen8Xuliang Cai9Yu Zhang10Riken Chen11Qingling Zhang12State Key Laboratory of Respiratory Diseases, Department of Pulmonary and Critical Care Medicine, Guangzhou Institute of Respiratory Health, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Rehabilitation Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaState Key Laboratory of Respiratory Diseases, Department of Pulmonary and Critical Care Medicine, Guangzhou Institute of Respiratory Health, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaState Key Laboratory of Respiratory Diseases, Department of Pulmonary and Critical Care Medicine, Guangzhou Institute of Respiratory Health, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaState Key Laboratory of Respiratory Diseases, Department of Pulmonary and Critical Care Medicine, Guangzhou Institute of Respiratory Health, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaState Key Laboratory of Respiratory Diseases, Department of Pulmonary and Critical Care Medicine, Guangzhou Institute of Respiratory Health, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Pediatric Hematology, Affiliated Hospital of Guizhou Medical University, Guiyang, ChinaDepartment of Rehabilitation, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, ChinaState Key Laboratory of Respiratory Diseases, Department of Pulmonary and Critical Care Medicine, Guangzhou Institute of Respiratory Health, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaState Key Laboratory of Respiratory Diseases, Department of Pulmonary and Critical Care Medicine, Guangzhou Institute of Respiratory Health, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Critical Care Medicine, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaState Key Laboratory of Respiratory Diseases, Department of Pulmonary and Critical Care Medicine, Guangzhou Institute of Respiratory Health, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaState Key Laboratory of Respiratory Diseases, Department of Pulmonary and Critical Care Medicine, Guangzhou Institute of Respiratory Health, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaEosinophilic asthma is the predominant phenotype of asthma, and although these patients are sensitive to glucocorticoid therapy, they also experience many side effects. Lonicerin is a kind of bioflavonoid isolated from the Chinese herb Lonicera japonica Thunb, which has anti-inflammatory and immunomodulatory effects. The aim of this study was to elucidate the effects of lonicerin on eosinophilic asthma and its potential mechanisms. Here, we established a house dust mite (house dust mite)-induced eosinophilic asthma model in BALB/c mouse, and evaluated the effects of lonicerin on it. Our results showed that lonicerin significantly reduced airway hyperresponsiveness the number of inflammatory cells (especially eosinophils) and the elevation of interleukin (IL)-4, IL-5, IL-13 and eotaxin in bronchoalveolar lavage fluid (BALF) supernatants of mice. Additionally, lonicerin also eminently blunted inflammatory infiltration and mucus secretion, as well as mRNA levels of Mucin 5AC (MUC5AC) in lung tissue. Furthermore, results of network pharmacology and molecular docking revealed that Src kinase and epidermal growth factor receptor may be the potential targets responsible for the effects of lonicerin. Finally, in vivo experiments confirmed that lonicerin inhibited activation of the Src/EGFR pathway by decreasing their phosphorylation. Taken together, the present study demonstrated that lonicerin could suppress HDM-induced eosinophilic asthma in mice through inhibiting the activation of Src/EGFR pathway, which also provides a basis for further research as a new potentially therapeutic agent for eosinophilic asthma and its underlying mechanisms in the future.https://www.frontiersin.org/articles/10.3389/fphar.2022.1051344/fullasthmaeosinophillonicerinnetwork pharmacologymolecular dockingsrc |
| spellingShingle | Zhenan Deng Xuefei Zhang Junjie Wen Xiaojing Yang Lingna Xue Changxing Ou Jianjuan Ma Hongrui Zhan Xiaomin Cen Xuliang Cai Yu Zhang Riken Chen Qingling Zhang Lonicerin attenuates house dust mite-induced eosinophilic asthma through targeting Src/EGFR signaling Frontiers in Pharmacology asthma eosinophil lonicerin network pharmacology molecular docking src |
| title | Lonicerin attenuates house dust mite-induced eosinophilic asthma through targeting Src/EGFR signaling |
| title_full | Lonicerin attenuates house dust mite-induced eosinophilic asthma through targeting Src/EGFR signaling |
| title_fullStr | Lonicerin attenuates house dust mite-induced eosinophilic asthma through targeting Src/EGFR signaling |
| title_full_unstemmed | Lonicerin attenuates house dust mite-induced eosinophilic asthma through targeting Src/EGFR signaling |
| title_short | Lonicerin attenuates house dust mite-induced eosinophilic asthma through targeting Src/EGFR signaling |
| title_sort | lonicerin attenuates house dust mite induced eosinophilic asthma through targeting src egfr signaling |
| topic | asthma eosinophil lonicerin network pharmacology molecular docking src |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.1051344/full |
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