Hybrid Genotype of <i>Anisakis simplex</i> (s.s.) and <i>A. pegreffii</i> Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine Waters
The sibling species <i>Anisakis simplex </i>(s.s.) and <i>Anisakis pegreffii </i>are parasites of marine mammals and fish worldwide and the main causative agents of human anisakiasis. In sympatric areas, a hybrid genotype between the two species has been identified, mainly in...
| Главные авторы: | , , , , |
|---|---|
| Формат: | Статья |
| Язык: | English |
| Опубликовано: |
MDPI AG
2021-08-01
|
| Серии: | Animals |
| Предметы: | |
| Online-ссылка: | https://www.mdpi.com/2076-2615/11/8/2458 |
| _version_ | 1827686146435448832 |
|---|---|
| author | Xavier Roca-Geronès M. Magdalena Alcover Carla Godínez-González Isabel Montoliu Roser Fisa |
| author_facet | Xavier Roca-Geronès M. Magdalena Alcover Carla Godínez-González Isabel Montoliu Roser Fisa |
| author_sort | Xavier Roca-Geronès |
| collection | DOAJ |
| description | The sibling species <i>Anisakis simplex </i>(s.s.) and <i>Anisakis pegreffii </i>are parasites of marine mammals and fish worldwide and the main causative agents of human anisakiasis. In sympatric areas, a hybrid genotype between the two species has been identified, mainly in third-stage larvae, but rarely in fourth-stage and adult forms. The aim of this study was to confirm the presence of hybrid genotypes in larvae parasitizing fish caught in sympatric and allopatric Spanish marine waters, the North-East Atlantic and West Mediterranean, respectively, and to study possible differences in the growth behaviour between genotypes. Of the 254 molecularly analysed larvae, 18 were identified as hybrids by PCR-RFLP analysis of the rDNA ITS region, 11 of which were subsequently confirmed by EF1 α-1 nDNA gene sequencing. These results therefore indicate an overestimation of hybrid genotypes when identification is based only on the ITS region. We also report the detection of a hybrid specimen in a host from the West Mediterranean, considered an allopatric zone. Additionally, fourth-stage larvae with a hybrid genotype were obtained in vitro for the first time, and no differences were observed in their growth behaviour compared to larvae with <i>A. simplex </i>(s.s.) and <i>A. pegreffii</i> genotypes. |
| first_indexed | 2024-03-10T09:04:17Z |
| format | Article |
| id | doaj.art-5e0c0f1b51ba4aa3af65a0b05c39ef88 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T09:04:17Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-5e0c0f1b51ba4aa3af65a0b05c39ef882023-11-22T06:32:28ZengMDPI AGAnimals2076-26152021-08-01118245810.3390/ani11082458Hybrid Genotype of <i>Anisakis simplex</i> (s.s.) and <i>A. pegreffii</i> Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine WatersXavier Roca-Geronès0M. Magdalena Alcover1Carla Godínez-González2Isabel Montoliu3Roser Fisa4Laboratory of Parasitology, Department of Biology, Health and Environment, Faculty of Pharmacy and Food Sciences, University of Barcelona, Av. Joan XXIII 27-31, 08028 Barcelona, SpainLaboratory of Parasitology, Department of Biology, Health and Environment, Faculty of Pharmacy and Food Sciences, University of Barcelona, Av. Joan XXIII 27-31, 08028 Barcelona, SpainLaboratory of Parasitology, Department of Biology, Health and Environment, Faculty of Pharmacy and Food Sciences, University of Barcelona, Av. Joan XXIII 27-31, 08028 Barcelona, SpainLaboratory of Parasitology, Department of Biology, Health and Environment, Faculty of Pharmacy and Food Sciences, University of Barcelona, Av. Joan XXIII 27-31, 08028 Barcelona, SpainLaboratory of Parasitology, Department of Biology, Health and Environment, Faculty of Pharmacy and Food Sciences, University of Barcelona, Av. Joan XXIII 27-31, 08028 Barcelona, SpainThe sibling species <i>Anisakis simplex </i>(s.s.) and <i>Anisakis pegreffii </i>are parasites of marine mammals and fish worldwide and the main causative agents of human anisakiasis. In sympatric areas, a hybrid genotype between the two species has been identified, mainly in third-stage larvae, but rarely in fourth-stage and adult forms. The aim of this study was to confirm the presence of hybrid genotypes in larvae parasitizing fish caught in sympatric and allopatric Spanish marine waters, the North-East Atlantic and West Mediterranean, respectively, and to study possible differences in the growth behaviour between genotypes. Of the 254 molecularly analysed larvae, 18 were identified as hybrids by PCR-RFLP analysis of the rDNA ITS region, 11 of which were subsequently confirmed by EF1 α-1 nDNA gene sequencing. These results therefore indicate an overestimation of hybrid genotypes when identification is based only on the ITS region. We also report the detection of a hybrid specimen in a host from the West Mediterranean, considered an allopatric zone. Additionally, fourth-stage larvae with a hybrid genotype were obtained in vitro for the first time, and no differences were observed in their growth behaviour compared to larvae with <i>A. simplex </i>(s.s.) and <i>A. pegreffii</i> genotypes.https://www.mdpi.com/2076-2615/11/8/2458<i>Anisakis simplex </i>(s.s.)<i>Anisakis pegreffii</i>hybrid genotypein vitro cultureSpanish marine watersgenotyping approach |
| spellingShingle | Xavier Roca-Geronès M. Magdalena Alcover Carla Godínez-González Isabel Montoliu Roser Fisa Hybrid Genotype of <i>Anisakis simplex</i> (s.s.) and <i>A. pegreffii</i> Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine Waters Animals <i>Anisakis simplex </i>(s.s.) <i>Anisakis pegreffii</i> hybrid genotype in vitro culture Spanish marine waters genotyping approach |
| title | Hybrid Genotype of <i>Anisakis simplex</i> (s.s.) and <i>A. pegreffii</i> Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine Waters |
| title_full | Hybrid Genotype of <i>Anisakis simplex</i> (s.s.) and <i>A. pegreffii</i> Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine Waters |
| title_fullStr | Hybrid Genotype of <i>Anisakis simplex</i> (s.s.) and <i>A. pegreffii</i> Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine Waters |
| title_full_unstemmed | Hybrid Genotype of <i>Anisakis simplex</i> (s.s.) and <i>A. pegreffii</i> Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine Waters |
| title_short | Hybrid Genotype of <i>Anisakis simplex</i> (s.s.) and <i>A. pegreffii</i> Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine Waters |
| title_sort | hybrid genotype of i anisakis simplex i s s and i a pegreffii i identified in third and fourth stage larvae from sympatric and allopatric spanish marine waters |
| topic | <i>Anisakis simplex </i>(s.s.) <i>Anisakis pegreffii</i> hybrid genotype in vitro culture Spanish marine waters genotyping approach |
| url | https://www.mdpi.com/2076-2615/11/8/2458 |
| work_keys_str_mv | AT xavierrocagerones hybridgenotypeofianisakissimplexissandiapegreffiiiidentifiedinthirdandfourthstagelarvaefromsympatricandallopatricspanishmarinewaters AT mmagdalenaalcover hybridgenotypeofianisakissimplexissandiapegreffiiiidentifiedinthirdandfourthstagelarvaefromsympatricandallopatricspanishmarinewaters AT carlagodinezgonzalez hybridgenotypeofianisakissimplexissandiapegreffiiiidentifiedinthirdandfourthstagelarvaefromsympatricandallopatricspanishmarinewaters AT isabelmontoliu hybridgenotypeofianisakissimplexissandiapegreffiiiidentifiedinthirdandfourthstagelarvaefromsympatricandallopatricspanishmarinewaters AT roserfisa hybridgenotypeofianisakissimplexissandiapegreffiiiidentifiedinthirdandfourthstagelarvaefromsympatricandallopatricspanishmarinewaters |