Genome-wide functional analyses of plant coiled-coil NLR-type pathogen receptors reveal essential roles of their N-terminal domain in oligomerization, networking, and immunity.
The ability to induce a defense response after pathogen attack is a critical feature of the immune system of any organism. Nucleotide-binding leucine-rich repeat receptors (NLRs) are key players in this process and perceive the occurrence of nonself-activities or foreign molecules. In plants, coevol...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-12-01
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| Series: | PLoS Biology |
| Online Access: | https://doi.org/10.1371/journal.pbio.2005821 |
| _version_ | 1818926923030462464 |
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| author | Tadeusz Wróblewski Laurentiu Spiridon Eliza Cristina Martin Andrei-Jose Petrescu Keri Cavanaugh Maria José Truco Huaqin Xu Dariusz Gozdowski Krzysztof Pawłowski Richard W Michelmore Frank L W Takken |
| author_facet | Tadeusz Wróblewski Laurentiu Spiridon Eliza Cristina Martin Andrei-Jose Petrescu Keri Cavanaugh Maria José Truco Huaqin Xu Dariusz Gozdowski Krzysztof Pawłowski Richard W Michelmore Frank L W Takken |
| author_sort | Tadeusz Wróblewski |
| collection | DOAJ |
| description | The ability to induce a defense response after pathogen attack is a critical feature of the immune system of any organism. Nucleotide-binding leucine-rich repeat receptors (NLRs) are key players in this process and perceive the occurrence of nonself-activities or foreign molecules. In plants, coevolution with a variety of pests and pathogens has resulted in repertoires of several hundred diverse NLRs in single individuals and many more in populations as a whole. However, the mechanism by which defense signaling is triggered by these NLRs in plants is poorly understood. Here, we show that upon pathogen perception, NLRs use their N-terminal domains to transactivate other receptors. Their N-terminal domains homo- and heterodimerize, suggesting that plant NLRs oligomerize upon activation, similar to the vertebrate NLRs; however, consistent with their large number in plants, the complexes are highly heterometric. Also, in contrast to metazoan NLRs, the N-terminus, rather than their centrally located nucleotide-binding (NB) domain, can mediate initial partner selection. The highly redundant network of NLR interactions in plants is proposed to provide resilience to perturbation by pathogens. |
| first_indexed | 2024-12-20T03:04:49Z |
| format | Article |
| id | doaj.art-5e0c56211c954b07919831958f375eb9 |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-20T03:04:49Z |
| publishDate | 2018-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-5e0c56211c954b07919831958f375eb92022-12-21T19:55:38ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852018-12-011612e200582110.1371/journal.pbio.2005821Genome-wide functional analyses of plant coiled-coil NLR-type pathogen receptors reveal essential roles of their N-terminal domain in oligomerization, networking, and immunity.Tadeusz WróblewskiLaurentiu SpiridonEliza Cristina MartinAndrei-Jose PetrescuKeri CavanaughMaria José TrucoHuaqin XuDariusz GozdowskiKrzysztof PawłowskiRichard W MichelmoreFrank L W TakkenThe ability to induce a defense response after pathogen attack is a critical feature of the immune system of any organism. Nucleotide-binding leucine-rich repeat receptors (NLRs) are key players in this process and perceive the occurrence of nonself-activities or foreign molecules. In plants, coevolution with a variety of pests and pathogens has resulted in repertoires of several hundred diverse NLRs in single individuals and many more in populations as a whole. However, the mechanism by which defense signaling is triggered by these NLRs in plants is poorly understood. Here, we show that upon pathogen perception, NLRs use their N-terminal domains to transactivate other receptors. Their N-terminal domains homo- and heterodimerize, suggesting that plant NLRs oligomerize upon activation, similar to the vertebrate NLRs; however, consistent with their large number in plants, the complexes are highly heterometric. Also, in contrast to metazoan NLRs, the N-terminus, rather than their centrally located nucleotide-binding (NB) domain, can mediate initial partner selection. The highly redundant network of NLR interactions in plants is proposed to provide resilience to perturbation by pathogens.https://doi.org/10.1371/journal.pbio.2005821 |
| spellingShingle | Tadeusz Wróblewski Laurentiu Spiridon Eliza Cristina Martin Andrei-Jose Petrescu Keri Cavanaugh Maria José Truco Huaqin Xu Dariusz Gozdowski Krzysztof Pawłowski Richard W Michelmore Frank L W Takken Genome-wide functional analyses of plant coiled-coil NLR-type pathogen receptors reveal essential roles of their N-terminal domain in oligomerization, networking, and immunity. PLoS Biology |
| title | Genome-wide functional analyses of plant coiled-coil NLR-type pathogen receptors reveal essential roles of their N-terminal domain in oligomerization, networking, and immunity. |
| title_full | Genome-wide functional analyses of plant coiled-coil NLR-type pathogen receptors reveal essential roles of their N-terminal domain in oligomerization, networking, and immunity. |
| title_fullStr | Genome-wide functional analyses of plant coiled-coil NLR-type pathogen receptors reveal essential roles of their N-terminal domain in oligomerization, networking, and immunity. |
| title_full_unstemmed | Genome-wide functional analyses of plant coiled-coil NLR-type pathogen receptors reveal essential roles of their N-terminal domain in oligomerization, networking, and immunity. |
| title_short | Genome-wide functional analyses of plant coiled-coil NLR-type pathogen receptors reveal essential roles of their N-terminal domain in oligomerization, networking, and immunity. |
| title_sort | genome wide functional analyses of plant coiled coil nlr type pathogen receptors reveal essential roles of their n terminal domain in oligomerization networking and immunity |
| url | https://doi.org/10.1371/journal.pbio.2005821 |
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