Aptamer Based Microsphere Biosensor for Thrombin Detection
We have developed an optical microsphere resonator biosensor using aptamer asreceptor for the measurement of the important biomolecule thrombin. The sphere surface ismodified with anti-thrombin aptamer, which has excellent binding affinity and selectivityfor thrombin. Binding of the thrombin at the...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2006-08-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/6/8/785/ |
| _version_ | 1828155324498968576 |
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| author | Xudong Fan Ian M. White Jonathan D. Suter Hongying Zhu |
| author_facet | Xudong Fan Ian M. White Jonathan D. Suter Hongying Zhu |
| author_sort | Xudong Fan |
| collection | DOAJ |
| description | We have developed an optical microsphere resonator biosensor using aptamer asreceptor for the measurement of the important biomolecule thrombin. The sphere surface ismodified with anti-thrombin aptamer, which has excellent binding affinity and selectivityfor thrombin. Binding of the thrombin at the sphere surface is monitored by the spectralposition of the microsphere’s whispering gallery mode resonances. A detection limit on theorder of 1 NIH Unit/mL is demonstrated. Control experiments with non-aptameroligonucleotide and BSA are also carried out to confirm the specific binding betweenaptamer and thrombin. We expect that this demonstration will lead to the development ofhighly sensitive biomarker sensors based on aptamer with lower cost and higher throughputthan current technology. |
| first_indexed | 2024-04-11T22:51:44Z |
| format | Article |
| id | doaj.art-5e0faaa5969748b08441c79c7a478cd6 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T22:51:44Z |
| publishDate | 2006-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-5e0faaa5969748b08441c79c7a478cd62022-12-22T03:58:33ZengMDPI AGSensors1424-82202006-08-016878579510.3390/s6080785Aptamer Based Microsphere Biosensor for Thrombin DetectionXudong FanIan M. WhiteJonathan D. SuterHongying ZhuWe have developed an optical microsphere resonator biosensor using aptamer asreceptor for the measurement of the important biomolecule thrombin. The sphere surface ismodified with anti-thrombin aptamer, which has excellent binding affinity and selectivityfor thrombin. Binding of the thrombin at the sphere surface is monitored by the spectralposition of the microsphere’s whispering gallery mode resonances. A detection limit on theorder of 1 NIH Unit/mL is demonstrated. Control experiments with non-aptameroligonucleotide and BSA are also carried out to confirm the specific binding betweenaptamer and thrombin. We expect that this demonstration will lead to the development ofhighly sensitive biomarker sensors based on aptamer with lower cost and higher throughputthan current technology.http://www.mdpi.com/1424-8220/6/8/785/Microspherering resonatorwhispering gallery modeaptamerthrombin |
| spellingShingle | Xudong Fan Ian M. White Jonathan D. Suter Hongying Zhu Aptamer Based Microsphere Biosensor for Thrombin Detection Sensors Microsphere ring resonator whispering gallery mode aptamer thrombin |
| title | Aptamer Based Microsphere Biosensor for Thrombin Detection |
| title_full | Aptamer Based Microsphere Biosensor for Thrombin Detection |
| title_fullStr | Aptamer Based Microsphere Biosensor for Thrombin Detection |
| title_full_unstemmed | Aptamer Based Microsphere Biosensor for Thrombin Detection |
| title_short | Aptamer Based Microsphere Biosensor for Thrombin Detection |
| title_sort | aptamer based microsphere biosensor for thrombin detection |
| topic | Microsphere ring resonator whispering gallery mode aptamer thrombin |
| url | http://www.mdpi.com/1424-8220/6/8/785/ |
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