Butanol Fraction Inhibits MCF-7 Breast Cancer Cell Migration, Adhesion, and Invasiveness
In this study, the potential of an n-butanol fraction from Ricinus communis to prevent metastasis in MCF-7 breast cancer cells was investigated. The effect of the fraction on BUD-8 and MCF-7 cell viability was assessed using the MTT assay. Apoptotic cell death was analyzed by Hoechst staining assay....
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2021-02-01
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| Series: | Integrative Cancer Therapies |
| Online Access: | https://doi.org/10.1177/1534735420977684 |
| _version_ | 1819240227915431936 |
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| author | Rixile Mabasa MSc Kholofelo Malemela MSc Karabo Serala MSc Mante Kgakishe BSc (Hons.) Thabe Matsebatlela PhD Matlou Mokgotho PhD Vusi Mbazima PhD |
| author_facet | Rixile Mabasa MSc Kholofelo Malemela MSc Karabo Serala MSc Mante Kgakishe BSc (Hons.) Thabe Matsebatlela PhD Matlou Mokgotho PhD Vusi Mbazima PhD |
| author_sort | Rixile Mabasa MSc |
| collection | DOAJ |
| description | In this study, the potential of an n-butanol fraction from Ricinus communis to prevent metastasis in MCF-7 breast cancer cells was investigated. The effect of the fraction on BUD-8 and MCF-7 cell viability was assessed using the MTT assay. Apoptotic cell death was analyzed by Hoechst staining assay. The antimetastatic effect of the fraction on MCF-7 cell was evaluated using the wound healing, adhesion and Boyden chamber invasion assays. Gelatin-zymography was used to assess the effect of the fraction on MMP-2 and MMP-9 activity. The expression profile of proteins implicated in metastasis and angiogenesis was determined using the human angiogenesis antibody array kit, following treatment with the fraction. BUD-8 cell viability was significantly reduced at concentrations between 300 and 500 µg/ml of the extract. In contrast, a significant reduction in cell viability was seen in MCF-7 cells treated with 400 to 500 µg/ml of the fraction. At sub-lethal concentrations (100 and 200 µg/ml) of the fraction, no nuclei morphological changes associated with apoptotic cell death were observed in MCF-7 cells. In addition, the fraction showed to have an inhibitory effect on MCF-7 cell migration, adhesion, invasiveness, and MMP-2 activity. Moreover, the fraction was seen to modulate the expression of several proteins, such as MMP-9, uPA, VEGF, and TGF-β1, playing a role in the metastasis process. This study demonstrates that the n -butanol fraction of R. communis can inhibit major steps of the metastatic cascade and modulate metastasis regulatory proteins. Thus, the fraction can be considered a potential source of antimetastatic agents that could be useful in the treatment of malignant cancers. |
| first_indexed | 2024-12-23T14:04:40Z |
| format | Article |
| id | doaj.art-5e1209f1c9784e73b851bfce57978650 |
| institution | Directory Open Access Journal |
| issn | 1534-7354 1552-695X |
| language | English |
| last_indexed | 2024-12-23T14:04:40Z |
| publishDate | 2021-02-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Integrative Cancer Therapies |
| spelling | doaj.art-5e1209f1c9784e73b851bfce579786502022-12-21T17:44:12ZengSAGE PublishingIntegrative Cancer Therapies1534-73541552-695X2021-02-012010.1177/1534735420977684Butanol Fraction Inhibits MCF-7 Breast Cancer Cell Migration, Adhesion, and InvasivenessRixile Mabasa MSc0Kholofelo Malemela MSc1Karabo Serala MSc2Mante Kgakishe BSc (Hons.)3Thabe Matsebatlela PhD4Matlou Mokgotho PhD5Vusi Mbazima PhD6University of Limpopo, Sovenga, South AfricaUniversity of Limpopo, Sovenga, South AfricaUniversity of Limpopo, Sovenga, South AfricaUniversity of Limpopo, Sovenga, South AfricaUniversity of Limpopo, Sovenga, South AfricaUniversity of Limpopo, Sovenga, South AfricaUniversity of Limpopo, Sovenga, South AfricaIn this study, the potential of an n-butanol fraction from Ricinus communis to prevent metastasis in MCF-7 breast cancer cells was investigated. The effect of the fraction on BUD-8 and MCF-7 cell viability was assessed using the MTT assay. Apoptotic cell death was analyzed by Hoechst staining assay. The antimetastatic effect of the fraction on MCF-7 cell was evaluated using the wound healing, adhesion and Boyden chamber invasion assays. Gelatin-zymography was used to assess the effect of the fraction on MMP-2 and MMP-9 activity. The expression profile of proteins implicated in metastasis and angiogenesis was determined using the human angiogenesis antibody array kit, following treatment with the fraction. BUD-8 cell viability was significantly reduced at concentrations between 300 and 500 µg/ml of the extract. In contrast, a significant reduction in cell viability was seen in MCF-7 cells treated with 400 to 500 µg/ml of the fraction. At sub-lethal concentrations (100 and 200 µg/ml) of the fraction, no nuclei morphological changes associated with apoptotic cell death were observed in MCF-7 cells. In addition, the fraction showed to have an inhibitory effect on MCF-7 cell migration, adhesion, invasiveness, and MMP-2 activity. Moreover, the fraction was seen to modulate the expression of several proteins, such as MMP-9, uPA, VEGF, and TGF-β1, playing a role in the metastasis process. This study demonstrates that the n -butanol fraction of R. communis can inhibit major steps of the metastatic cascade and modulate metastasis regulatory proteins. Thus, the fraction can be considered a potential source of antimetastatic agents that could be useful in the treatment of malignant cancers.https://doi.org/10.1177/1534735420977684 |
| spellingShingle | Rixile Mabasa MSc Kholofelo Malemela MSc Karabo Serala MSc Mante Kgakishe BSc (Hons.) Thabe Matsebatlela PhD Matlou Mokgotho PhD Vusi Mbazima PhD Butanol Fraction Inhibits MCF-7 Breast Cancer Cell Migration, Adhesion, and Invasiveness Integrative Cancer Therapies |
| title | Butanol Fraction Inhibits MCF-7 Breast Cancer Cell Migration, Adhesion, and Invasiveness |
| title_full | Butanol Fraction Inhibits MCF-7 Breast Cancer Cell Migration, Adhesion, and Invasiveness |
| title_fullStr | Butanol Fraction Inhibits MCF-7 Breast Cancer Cell Migration, Adhesion, and Invasiveness |
| title_full_unstemmed | Butanol Fraction Inhibits MCF-7 Breast Cancer Cell Migration, Adhesion, and Invasiveness |
| title_short | Butanol Fraction Inhibits MCF-7 Breast Cancer Cell Migration, Adhesion, and Invasiveness |
| title_sort | butanol fraction inhibits mcf 7 breast cancer cell migration adhesion and invasiveness |
| url | https://doi.org/10.1177/1534735420977684 |
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