Nanofluidic Immobilization and Growth Detection of <em>Escherichia coli</em> in a Chip for Antibiotic Susceptibility Testing
Infections with antimicrobial resistant bacteria are a rising threat for global healthcare as more and more antibiotics lose their effectiveness against bacterial pathogens. To guarantee the long-term effectiveness of broad-spectrum antibiotics, they may only be prescribed when inevitably required....
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/10/10/135 |
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| author | Jan F. Busche Svenja Möller Ann-Kathrin Klein Matthias Stehr Foelke Purr Margherita Bassu Thomas P. Burg Andreas Dietzel |
| author_facet | Jan F. Busche Svenja Möller Ann-Kathrin Klein Matthias Stehr Foelke Purr Margherita Bassu Thomas P. Burg Andreas Dietzel |
| author_sort | Jan F. Busche |
| collection | DOAJ |
| description | Infections with antimicrobial resistant bacteria are a rising threat for global healthcare as more and more antibiotics lose their effectiveness against bacterial pathogens. To guarantee the long-term effectiveness of broad-spectrum antibiotics, they may only be prescribed when inevitably required. In order to make a reliable assessment of which antibiotics are effective, rapid point-of-care tests are needed. This can be achieved with fast phenotypic microfluidic tests, which can cope with low bacterial concentrations and work label-free. Here, we present a novel optofluidic chip with a cross-flow immobilization principle using a regular array of nanogaps to concentrate bacteria and detect their growth label-free under the influence of antibiotics. The interferometric measuring principle enabled the detection of the growth of <i>Escherichia coli</i> in under 4 h with a sample volume of 187.2 µL and a doubling time of 79 min. In proof-of-concept experiments, we could show that the method can distinguish between bacterial growth and its inhibition by antibiotics. The results indicate that the nanofluidic chip approach provides a very promising concept for future rapid and label-free antimicrobial susceptibility tests. |
| first_indexed | 2024-03-10T16:03:36Z |
| format | Article |
| id | doaj.art-5e12c40ba92b4b4aaceffc713e2b33ed |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T16:03:36Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-5e12c40ba92b4b4aaceffc713e2b33ed2023-11-20T15:04:42ZengMDPI AGBiosensors2079-63742020-09-01101013510.3390/bios10100135Nanofluidic Immobilization and Growth Detection of <em>Escherichia coli</em> in a Chip for Antibiotic Susceptibility TestingJan F. Busche0Svenja Möller1Ann-Kathrin Klein2Matthias Stehr3Foelke Purr4Margherita Bassu5Thomas P. Burg6Andreas Dietzel7Institute of Microtechnology, Technische Universität Braunschweig, 38124 Braunschweig, GermanyInstitute of Microtechnology, Technische Universität Braunschweig, 38124 Braunschweig, GermanyInstitute of Microtechnology, Technische Universität Braunschweig, 38124 Braunschweig, GermanyLionex Diagnostics & Therapeutics GmbH, 38124 Braunschweig, GermanyInstitute of Microtechnology, Technische Universität Braunschweig, 38124 Braunschweig, GermanyMax Planck Institute for Biophysical Chemistry, 37077 Göttingen, GermanyMax Planck Institute for Biophysical Chemistry, 37077 Göttingen, GermanyInstitute of Microtechnology, Technische Universität Braunschweig, 38124 Braunschweig, GermanyInfections with antimicrobial resistant bacteria are a rising threat for global healthcare as more and more antibiotics lose their effectiveness against bacterial pathogens. To guarantee the long-term effectiveness of broad-spectrum antibiotics, they may only be prescribed when inevitably required. In order to make a reliable assessment of which antibiotics are effective, rapid point-of-care tests are needed. This can be achieved with fast phenotypic microfluidic tests, which can cope with low bacterial concentrations and work label-free. Here, we present a novel optofluidic chip with a cross-flow immobilization principle using a regular array of nanogaps to concentrate bacteria and detect their growth label-free under the influence of antibiotics. The interferometric measuring principle enabled the detection of the growth of <i>Escherichia coli</i> in under 4 h with a sample volume of 187.2 µL and a doubling time of 79 min. In proof-of-concept experiments, we could show that the method can distinguish between bacterial growth and its inhibition by antibiotics. The results indicate that the nanofluidic chip approach provides a very promising concept for future rapid and label-free antimicrobial susceptibility tests.https://www.mdpi.com/2079-6374/10/10/135optofluidicnanofluidicantibiotic resistance testnano-gratingmicrofabrication |
| spellingShingle | Jan F. Busche Svenja Möller Ann-Kathrin Klein Matthias Stehr Foelke Purr Margherita Bassu Thomas P. Burg Andreas Dietzel Nanofluidic Immobilization and Growth Detection of <em>Escherichia coli</em> in a Chip for Antibiotic Susceptibility Testing Biosensors optofluidic nanofluidic antibiotic resistance test nano-grating microfabrication |
| title | Nanofluidic Immobilization and Growth Detection of <em>Escherichia coli</em> in a Chip for Antibiotic Susceptibility Testing |
| title_full | Nanofluidic Immobilization and Growth Detection of <em>Escherichia coli</em> in a Chip for Antibiotic Susceptibility Testing |
| title_fullStr | Nanofluidic Immobilization and Growth Detection of <em>Escherichia coli</em> in a Chip for Antibiotic Susceptibility Testing |
| title_full_unstemmed | Nanofluidic Immobilization and Growth Detection of <em>Escherichia coli</em> in a Chip for Antibiotic Susceptibility Testing |
| title_short | Nanofluidic Immobilization and Growth Detection of <em>Escherichia coli</em> in a Chip for Antibiotic Susceptibility Testing |
| title_sort | nanofluidic immobilization and growth detection of em escherichia coli em in a chip for antibiotic susceptibility testing |
| topic | optofluidic nanofluidic antibiotic resistance test nano-grating microfabrication |
| url | https://www.mdpi.com/2079-6374/10/10/135 |
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