Anti-angiogenic mechanism of cordycepin on rhesus macaque choroid-retinal endothelial cell line cultured in high glucose condition
AIM: To investigate the angiogenesis effect and protective mechanism of cordycepin on rhesus macaque choroid-retinal endothelial(RF/6A)cell line cultured in high glucose condition. <p>METHODS: Cultured RF/6A cells were divided into normal control group, high glucose group and high glucose(HG)+...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2016-07-01
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| Series: | Guoji Yanke Zazhi |
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| Online Access: | http://ies.ijo.cn/cn_publish/2016/7/201607008.pdf |
| _version_ | 1811277683191971840 |
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| author | Xiao-Li Zhu<sup>*</sup> Xiao-Feng Sun<sup>*</sup> Ming-Ying Lai |
| author_facet | Xiao-Li Zhu<sup>*</sup> Xiao-Feng Sun<sup>*</sup> Ming-Ying Lai |
| author_sort | Xiao-Li Zhu<sup>*</sup> |
| collection | DOAJ |
| description | AIM: To investigate the angiogenesis effect and protective mechanism of cordycepin on rhesus macaque choroid-retinal endothelial(RF/6A)cell line cultured in high glucose condition. <p>METHODS: Cultured RF/6A cells were divided into normal control group, high glucose group and high glucose(HG)+ different concentration cordycepin groups(HG+10μg/mL group, HG+50μg/mL group, HG+100μg/mL group). The cell proliferation was assessed using cholecystokinin octapeptide dye after treated for 48h. The cell migration was investigated by a Transwell assay. The tube formation was measured on Matrigel. Furthermore, the impact of cordycepin on high glucose-induced activation of VEGF and VEGF receptor 2(VEGFR-2)was tested by Western blot analysis. <p>RESULTS: Compared with normal control group, cell viability markedly increased in high glucose group(<i>P</i><0.05). Cordycepin inhibited RF/6A cell proliferation in a dose-dependent fashion: 10.2±0.9%, 23.4±1.5% and 31.1±1.2% inhibition as the concentrations of cordycepin were 10, 50 and 100μg/mL, respectively. The difference had statistically significant(<i>P</i><0.05)compared with high glucose group. The number of cell migration were 55.6±2.70, 87.4±2.40, 65.4±2.7, 57.8±2.38, 62.4±2.77 in normal control group, high glucose group and HG+10μg/mL group, HG+50μg/mL group, HG+100μg/mL group respectively. Migration of RF/6A conspicuously increased in high glucose group(<i>P</i><0.05)compared with normal control group; while showing a gradually reducing trend with the increase of cordycepin dose and a statistically significant difference compared with high glucose group(<i>P</i><0.05). The number of tube formation were 18.7±2.08, 25.7±1.52, 19.9±1.57, 16.3± 2.51, 5.67±1.72 in the abovementioned group. Similarly showing a gradually reducing trend with the increase of cordycepin dose and a statistically significant difference with high glucose group(<i>P</i><0.05). In addition, the number of tube formation of RF/6A in high glucose group significant increased compared with normal control group(<i>P</i><0.05). The expression of VEGF and VEGFR-2 dramaticlly increased in high glucose group <i>vs</i> normal control group, oppositely gradually decreased with the increase of cordycepin concentrations, and had a statistically significant difference <i>vs</i> high glucose group(<i>P</i><0.05). <p>CONCLUSION: Cordycepin can suppress the proliferation, migration and tubu formation of RF/6A in high glucose condition, might via inhibiting expression of VEGF and VEGFR-2. |
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| format | Article |
| id | doaj.art-5e165d2a4f13471190c66f90113b11fc |
| institution | Directory Open Access Journal |
| issn | 1672-5123 1672-5123 |
| language | English |
| last_indexed | 2024-04-13T00:21:09Z |
| publishDate | 2016-07-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-5e165d2a4f13471190c66f90113b11fc2022-12-22T03:10:46ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232016-07-011671237124110.3980/j.issn.1672-5123.2016.7.08Anti-angiogenic mechanism of cordycepin on rhesus macaque choroid-retinal endothelial cell line cultured in high glucose conditionXiao-Li Zhu<sup>*</sup>0Xiao-Feng Sun<sup>*</sup>1Ming-Ying Lai2Shenzhen Eye Hospital Affiliated to Jinan University, Shenzhen Key Laboratory of Ophthalmology, Shenzhen 518000, Guangdong Province, ChinaCo-first authors:*:Xiao-Li Zhu and Xiao-Feng Sun.Shenzhen Eye Hospital Affiliated to Jinan University, Shenzhen Key Laboratory of Ophthalmology, Shenzhen 518000, Guangdong Province, ChinaCo-first authors:*:Xiao-Li Zhu and Xiao-Feng Sun.Shenzhen Eye Hospital Affiliated to Jinan University, Shenzhen Key Laboratory of Ophthalmology, Shenzhen 518000, Guangdong Province, ChinaCo-first authors:*:Xiao-Li Zhu and Xiao-Feng Sun.AIM: To investigate the angiogenesis effect and protective mechanism of cordycepin on rhesus macaque choroid-retinal endothelial(RF/6A)cell line cultured in high glucose condition. <p>METHODS: Cultured RF/6A cells were divided into normal control group, high glucose group and high glucose(HG)+ different concentration cordycepin groups(HG+10μg/mL group, HG+50μg/mL group, HG+100μg/mL group). The cell proliferation was assessed using cholecystokinin octapeptide dye after treated for 48h. The cell migration was investigated by a Transwell assay. The tube formation was measured on Matrigel. Furthermore, the impact of cordycepin on high glucose-induced activation of VEGF and VEGF receptor 2(VEGFR-2)was tested by Western blot analysis. <p>RESULTS: Compared with normal control group, cell viability markedly increased in high glucose group(<i>P</i><0.05). Cordycepin inhibited RF/6A cell proliferation in a dose-dependent fashion: 10.2±0.9%, 23.4±1.5% and 31.1±1.2% inhibition as the concentrations of cordycepin were 10, 50 and 100μg/mL, respectively. The difference had statistically significant(<i>P</i><0.05)compared with high glucose group. The number of cell migration were 55.6±2.70, 87.4±2.40, 65.4±2.7, 57.8±2.38, 62.4±2.77 in normal control group, high glucose group and HG+10μg/mL group, HG+50μg/mL group, HG+100μg/mL group respectively. Migration of RF/6A conspicuously increased in high glucose group(<i>P</i><0.05)compared with normal control group; while showing a gradually reducing trend with the increase of cordycepin dose and a statistically significant difference compared with high glucose group(<i>P</i><0.05). The number of tube formation were 18.7±2.08, 25.7±1.52, 19.9±1.57, 16.3± 2.51, 5.67±1.72 in the abovementioned group. Similarly showing a gradually reducing trend with the increase of cordycepin dose and a statistically significant difference with high glucose group(<i>P</i><0.05). In addition, the number of tube formation of RF/6A in high glucose group significant increased compared with normal control group(<i>P</i><0.05). The expression of VEGF and VEGFR-2 dramaticlly increased in high glucose group <i>vs</i> normal control group, oppositely gradually decreased with the increase of cordycepin concentrations, and had a statistically significant difference <i>vs</i> high glucose group(<i>P</i><0.05). <p>CONCLUSION: Cordycepin can suppress the proliferation, migration and tubu formation of RF/6A in high glucose condition, might via inhibiting expression of VEGF and VEGFR-2.http://ies.ijo.cn/cn_publish/2016/7/201607008.pdfCordycepinhigh glucose conditionrhesus macaque choroid-retinal endothelial(RF/6A)cell linevascular endothelial growth factorneovascularizationneovascularization |
| spellingShingle | Xiao-Li Zhu<sup>*</sup> Xiao-Feng Sun<sup>*</sup> Ming-Ying Lai Anti-angiogenic mechanism of cordycepin on rhesus macaque choroid-retinal endothelial cell line cultured in high glucose condition Guoji Yanke Zazhi Cordycepin high glucose condition rhesus macaque choroid-retinal endothelial(RF/6A)cell line vascular endothelial growth factor neovascularization neovascularization |
| title | Anti-angiogenic mechanism of cordycepin on rhesus macaque choroid-retinal endothelial cell line cultured in high glucose condition |
| title_full | Anti-angiogenic mechanism of cordycepin on rhesus macaque choroid-retinal endothelial cell line cultured in high glucose condition |
| title_fullStr | Anti-angiogenic mechanism of cordycepin on rhesus macaque choroid-retinal endothelial cell line cultured in high glucose condition |
| title_full_unstemmed | Anti-angiogenic mechanism of cordycepin on rhesus macaque choroid-retinal endothelial cell line cultured in high glucose condition |
| title_short | Anti-angiogenic mechanism of cordycepin on rhesus macaque choroid-retinal endothelial cell line cultured in high glucose condition |
| title_sort | anti angiogenic mechanism of cordycepin on rhesus macaque choroid retinal endothelial cell line cultured in high glucose condition |
| topic | Cordycepin high glucose condition rhesus macaque choroid-retinal endothelial(RF/6A)cell line vascular endothelial growth factor neovascularization neovascularization |
| url | http://ies.ijo.cn/cn_publish/2016/7/201607008.pdf |
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