The effect of rhinovirus on airway inflammation in a murine asthma model
PurposeThe aim of the present study was to investigate the differences in lower airway inflammatory immune responses, including cellular responses and responses in terms of inflammatory mediators in bronchoalveolar lavage fluid (BALF) and the airway, to rhinovirus (RV) infection on asthma exacerbati...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Korean Pediatric Society
2013-11-01
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| Series: | Korean Journal of Pediatrics |
| Subjects: | |
| Online Access: | http://kjp.or.kr/upload/pdf/kjped-56-482.pdf |
| _version_ | 1818210132769636352 |
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| author | Eugene Kim Huisu Lee Hyun Sook Kim Sulmui Won Eu Kyoung Lee Hwan Soo Kim Kyongwon Bang Yoon Hong Chun Jong-Seo Yoon Hyun Hee Kim Jin Tack Kim Joon Sung Lee |
| author_facet | Eugene Kim Huisu Lee Hyun Sook Kim Sulmui Won Eu Kyoung Lee Hwan Soo Kim Kyongwon Bang Yoon Hong Chun Jong-Seo Yoon Hyun Hee Kim Jin Tack Kim Joon Sung Lee |
| author_sort | Eugene Kim |
| collection | DOAJ |
| description | PurposeThe aim of the present study was to investigate the differences in lower airway inflammatory immune responses, including cellular responses and responses in terms of inflammatory mediators in bronchoalveolar lavage fluid (BALF) and the airway, to rhinovirus (RV) infection on asthma exacerbation by comparing a control and a murine asthma model, with or without RV infection.MethodsBALB/c mice were intraperitoneally injected with a crude extract of Dermatophagoides farinae (Df) or phosphate buffered saline (PBS) and were subsequently intranasally treated with a crude extract of Df or PBS. Airway responsiveness and cell infiltration, differential cell counts in BALF, and cytokine and chemokine concentrations in BALF were measured 24 hours after intranasal RV1B infection.ResultsRV infection increased the enhanced pause (Penh) in both the Df sensitized and challenged mice (Df mice) and PBS-treated mice (PBS mice) (P<0.05). Airway eosinophil infiltration increased in Df mice after RV infection (P<0.05). The levels of interleukin (IL) 13, tumor necrosis factor alpha, and regulated on activation, normal T cells expressed and secreted (RANTES) increased in response to RV infection in Df mice, but not in PBS mice (P<0.05). The level of IL-10 significantly decreased following RV infection in Df mice (P<0.05).ConclusionOur findings suggest that the augmented induction of proinflammatory cytokines, Th2 cytokines, and chemokines that mediate an eosinophil response and the decreased induction of regulatory cytokines after RV infection may be important manifestations leading to airway inflammation with eosinophil infiltration and changes in airway responsiveness in the asthma model. |
| first_indexed | 2024-12-12T05:11:45Z |
| format | Article |
| id | doaj.art-5e17b0f0a7914a12993990038d9ea292 |
| institution | Directory Open Access Journal |
| issn | 1738-1061 2092-7258 |
| language | English |
| last_indexed | 2024-12-12T05:11:45Z |
| publishDate | 2013-11-01 |
| publisher | Korean Pediatric Society |
| record_format | Article |
| series | Korean Journal of Pediatrics |
| spelling | doaj.art-5e17b0f0a7914a12993990038d9ea2922022-12-22T00:36:53ZengKorean Pediatric SocietyKorean Journal of Pediatrics1738-10612092-72582013-11-01561148248910.3345/kjp.2013.56.11.4822013600042The effect of rhinovirus on airway inflammation in a murine asthma modelEugene Kim0Huisu Lee1Hyun Sook Kim2Sulmui Won3Eu Kyoung Lee4Hwan Soo Kim5Kyongwon Bang6Yoon Hong Chun7Jong-Seo Yoon8Hyun Hee Kim9Jin Tack Kim10Joon Sung Lee11Department of Pediatrics, The Catholic University of Korea College of Medicine, Seoul, Korea.Department of Pediatrics, The Catholic University of Korea College of Medicine, Seoul, Korea.Department of Pediatrics, The Catholic University of Korea College of Medicine, Seoul, Korea.Department of Pediatrics, The Catholic University of Korea College of Medicine, Seoul, Korea.Department of Pediatrics, The Catholic University of Korea College of Medicine, Seoul, Korea.Department of Pediatrics, The Catholic University of Korea College of Medicine, Seoul, Korea.Department of Pediatrics, The Catholic University of Korea College of Medicine, Seoul, Korea.Department of Pediatrics, The Catholic University of Korea College of Medicine, Seoul, Korea.Department of Pediatrics, The Catholic University of Korea College of Medicine, Seoul, Korea.Department of Pediatrics, The Catholic University of Korea College of Medicine, Seoul, Korea.Department of Pediatrics, The Catholic University of Korea College of Medicine, Seoul, Korea.Department of Pediatrics, The Catholic University of Korea College of Medicine, Seoul, Korea.PurposeThe aim of the present study was to investigate the differences in lower airway inflammatory immune responses, including cellular responses and responses in terms of inflammatory mediators in bronchoalveolar lavage fluid (BALF) and the airway, to rhinovirus (RV) infection on asthma exacerbation by comparing a control and a murine asthma model, with or without RV infection.MethodsBALB/c mice were intraperitoneally injected with a crude extract of Dermatophagoides farinae (Df) or phosphate buffered saline (PBS) and were subsequently intranasally treated with a crude extract of Df or PBS. Airway responsiveness and cell infiltration, differential cell counts in BALF, and cytokine and chemokine concentrations in BALF were measured 24 hours after intranasal RV1B infection.ResultsRV infection increased the enhanced pause (Penh) in both the Df sensitized and challenged mice (Df mice) and PBS-treated mice (PBS mice) (P<0.05). Airway eosinophil infiltration increased in Df mice after RV infection (P<0.05). The levels of interleukin (IL) 13, tumor necrosis factor alpha, and regulated on activation, normal T cells expressed and secreted (RANTES) increased in response to RV infection in Df mice, but not in PBS mice (P<0.05). The level of IL-10 significantly decreased following RV infection in Df mice (P<0.05).ConclusionOur findings suggest that the augmented induction of proinflammatory cytokines, Th2 cytokines, and chemokines that mediate an eosinophil response and the decreased induction of regulatory cytokines after RV infection may be important manifestations leading to airway inflammation with eosinophil infiltration and changes in airway responsiveness in the asthma model.http://kjp.or.kr/upload/pdf/kjped-56-482.pdfImmune responseRhinovirusAsthmaExacerbation |
| spellingShingle | Eugene Kim Huisu Lee Hyun Sook Kim Sulmui Won Eu Kyoung Lee Hwan Soo Kim Kyongwon Bang Yoon Hong Chun Jong-Seo Yoon Hyun Hee Kim Jin Tack Kim Joon Sung Lee The effect of rhinovirus on airway inflammation in a murine asthma model Korean Journal of Pediatrics Immune response Rhinovirus Asthma Exacerbation |
| title | The effect of rhinovirus on airway inflammation in a murine asthma model |
| title_full | The effect of rhinovirus on airway inflammation in a murine asthma model |
| title_fullStr | The effect of rhinovirus on airway inflammation in a murine asthma model |
| title_full_unstemmed | The effect of rhinovirus on airway inflammation in a murine asthma model |
| title_short | The effect of rhinovirus on airway inflammation in a murine asthma model |
| title_sort | effect of rhinovirus on airway inflammation in a murine asthma model |
| topic | Immune response Rhinovirus Asthma Exacerbation |
| url | http://kjp.or.kr/upload/pdf/kjped-56-482.pdf |
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