Biomimetic In Vitro Model of Cell Infiltration into Skin Scaffolds for Pre-Screening and Testing of Biomaterial-Based Therapies
Due to great clinical need, research where different biomaterials are tested as 3D scaffolds for skin tissue engineering has increased. In vitro studies use a cell suspension that is simply pipetted onto the material and cultured until the cells migrate and proliferate within the 3D scaffold, which...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-08-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/8/8/917 |
| _version_ | 1797761642291789824 |
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| author | Rafael Ballesteros-Cillero Evan Davison-Kotler Nupur Kohli William S. Marshall Elena García-Gareta |
| author_facet | Rafael Ballesteros-Cillero Evan Davison-Kotler Nupur Kohli William S. Marshall Elena García-Gareta |
| author_sort | Rafael Ballesteros-Cillero |
| collection | DOAJ |
| description | Due to great clinical need, research where different biomaterials are tested as 3D scaffolds for skin tissue engineering has increased. In vitro studies use a cell suspension that is simply pipetted onto the material and cultured until the cells migrate and proliferate within the 3D scaffold, which does not mimic the in vivo reality. Our aim was to engineer a novel biomimetic in vitro model that mimics the natural cell infiltration process occurring in wound healing, thus offering a realistic approach when pre-screening and testing new skin substitutes. Our model consists of porous membrane cell culture inserts coated with gelatin and seeded with human dermal fibroblasts, inside which two different commercially available dermal substitutes were placed. Several features relevant to the wound healing process (matrix contraction, cell infiltration and proliferation, integration of the biomaterial with the surrounding tissue, and secretion of exogenous cytokines and growth factors) were evaluated. Our results showed that cells spontaneously infiltrate the materials and that our engineered model is able to induce and detect subtle differences between different biomaterials. The model allows for room for improvements or “adds-on” and miniaturization and can contribute to the development of functional and efficient skin substitutes for burns and chronic wounds. |
| first_indexed | 2024-03-12T19:16:06Z |
| format | Article |
| id | doaj.art-5e1e704874594f5e8955cdcb0dc85e9a |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T19:16:06Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-5e1e704874594f5e8955cdcb0dc85e9a2023-08-02T05:30:53ZengMDPI AGCells2073-44092019-08-018891710.3390/cells8080917cells8080917Biomimetic In Vitro Model of Cell Infiltration into Skin Scaffolds for Pre-Screening and Testing of Biomaterial-Based TherapiesRafael Ballesteros-Cillero0Evan Davison-Kotler1Nupur Kohli2William S. Marshall3Elena García-Gareta4Regenerative Biomaterials Group, RAFT Institute, Mount Vernon Hospital, Northwood HA6 2RN, UKRegenerative Biomaterials Group, RAFT Institute, Mount Vernon Hospital, Northwood HA6 2RN, UKRegenerative Biomaterials Group, RAFT Institute, Mount Vernon Hospital, Northwood HA6 2RN, UKBiology Department, St. Francis Xavier University, Antigonish, NS B2G 2W5, CanadaRegenerative Biomaterials Group, RAFT Institute, Mount Vernon Hospital, Northwood HA6 2RN, UKDue to great clinical need, research where different biomaterials are tested as 3D scaffolds for skin tissue engineering has increased. In vitro studies use a cell suspension that is simply pipetted onto the material and cultured until the cells migrate and proliferate within the 3D scaffold, which does not mimic the in vivo reality. Our aim was to engineer a novel biomimetic in vitro model that mimics the natural cell infiltration process occurring in wound healing, thus offering a realistic approach when pre-screening and testing new skin substitutes. Our model consists of porous membrane cell culture inserts coated with gelatin and seeded with human dermal fibroblasts, inside which two different commercially available dermal substitutes were placed. Several features relevant to the wound healing process (matrix contraction, cell infiltration and proliferation, integration of the biomaterial with the surrounding tissue, and secretion of exogenous cytokines and growth factors) were evaluated. Our results showed that cells spontaneously infiltrate the materials and that our engineered model is able to induce and detect subtle differences between different biomaterials. The model allows for room for improvements or “adds-on” and miniaturization and can contribute to the development of functional and efficient skin substitutes for burns and chronic wounds.https://www.mdpi.com/2073-4409/8/8/917cell infiltrationbiomimeticwound healingtissue engineeringskin substitutebiomaterial |
| spellingShingle | Rafael Ballesteros-Cillero Evan Davison-Kotler Nupur Kohli William S. Marshall Elena García-Gareta Biomimetic In Vitro Model of Cell Infiltration into Skin Scaffolds for Pre-Screening and Testing of Biomaterial-Based Therapies Cells cell infiltration biomimetic wound healing tissue engineering skin substitute biomaterial |
| title | Biomimetic In Vitro Model of Cell Infiltration into Skin Scaffolds for Pre-Screening and Testing of Biomaterial-Based Therapies |
| title_full | Biomimetic In Vitro Model of Cell Infiltration into Skin Scaffolds for Pre-Screening and Testing of Biomaterial-Based Therapies |
| title_fullStr | Biomimetic In Vitro Model of Cell Infiltration into Skin Scaffolds for Pre-Screening and Testing of Biomaterial-Based Therapies |
| title_full_unstemmed | Biomimetic In Vitro Model of Cell Infiltration into Skin Scaffolds for Pre-Screening and Testing of Biomaterial-Based Therapies |
| title_short | Biomimetic In Vitro Model of Cell Infiltration into Skin Scaffolds for Pre-Screening and Testing of Biomaterial-Based Therapies |
| title_sort | biomimetic in vitro model of cell infiltration into skin scaffolds for pre screening and testing of biomaterial based therapies |
| topic | cell infiltration biomimetic wound healing tissue engineering skin substitute biomaterial |
| url | https://www.mdpi.com/2073-4409/8/8/917 |
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