Green Synthesis of Narrow-Size Silver Nanoparticles Using <i>Ginkgo biloba</i> Leaves: Condition Optimization, Characterization, and Antibacterial and Cytotoxic Activities
Natural products derived from medicinal plants offer convenience and therapeutic potential and have inspired the development of antimicrobial agents. Thus, it is worth exploring the combination of nanotechnology and natural products. In this study, silver nanoparticles (AgNPs) were synthesized from...
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MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/3/1913 |
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| author | Qi Ni Ting Zhu Wenjie Wang Dongdong Guo Yixiao Li Tianyu Chen Xiaojun Zhang |
| author_facet | Qi Ni Ting Zhu Wenjie Wang Dongdong Guo Yixiao Li Tianyu Chen Xiaojun Zhang |
| author_sort | Qi Ni |
| collection | DOAJ |
| description | Natural products derived from medicinal plants offer convenience and therapeutic potential and have inspired the development of antimicrobial agents. Thus, it is worth exploring the combination of nanotechnology and natural products. In this study, silver nanoparticles (AgNPs) were synthesized from the leaf extract of <i>Ginkgo biloba</i> (Gb), having abundant flavonoid compounds. The reaction conditions and the colloidal stability were assessed using ultraviolet–visible spectroscopy. X-ray diffraction, transmission electron microscopy, and Fourier transform infrared spectroscopy (FTIR) were used to characterize the AgNPs. AgNPs exhibited a spherical morphology, uniform dispersion, and diameter ranging from ~8 to 9 nm. The FTIR data indicated that phytoconstituents, such as polyphenols, flavonoids, and terpenoids, could potentially serve as reducing and capping agents. The antibacterial activity of the synthesized AgNPs was assessed using broth dilution and agar well diffusion assays. The results demonstrate antibacterial effects against both Gram-positive and Gram-negative strains at low AgNP concentrations. The cytotoxicity of AgNPs was examined in vitro using the CCK-8 method, which showed that low concentrations of AgNPs are noncytotoxic to normal cells and promote cell growth. In conclusion, an environmentally friendly approach for synthesizing AgNPs from Gb leaves yielded antibacterial AgNPs with minimal toxicity, holding promise for future applications in the field of biomedicine. |
| first_indexed | 2024-03-08T03:54:38Z |
| format | Article |
| id | doaj.art-5e203ce13f104f7993a268b51e14519f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T03:54:38Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5e203ce13f104f7993a268b51e14519f2024-02-09T15:15:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01253191310.3390/ijms25031913Green Synthesis of Narrow-Size Silver Nanoparticles Using <i>Ginkgo biloba</i> Leaves: Condition Optimization, Characterization, and Antibacterial and Cytotoxic ActivitiesQi Ni0Ting Zhu1Wenjie Wang2Dongdong Guo3Yixiao Li4Tianyu Chen5Xiaojun Zhang6Key Laboratory of Resource Biology and Biotechnology Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi’an 710069, ChinaKey Laboratory of Resource Biology and Biotechnology Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi’an 710069, ChinaKey Laboratory of Resource Biology and Biotechnology Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi’an 710069, ChinaKey Laboratory of Resource Biology and Biotechnology Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi’an 710069, ChinaSchool of Medicine, Northwest University, 229 Taibai North Road, Xi’an 710069, ChinaKey Laboratory of Resource Biology and Biotechnology Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi’an 710069, ChinaSchool of Medicine, Northwest University, 229 Taibai North Road, Xi’an 710069, ChinaNatural products derived from medicinal plants offer convenience and therapeutic potential and have inspired the development of antimicrobial agents. Thus, it is worth exploring the combination of nanotechnology and natural products. In this study, silver nanoparticles (AgNPs) were synthesized from the leaf extract of <i>Ginkgo biloba</i> (Gb), having abundant flavonoid compounds. The reaction conditions and the colloidal stability were assessed using ultraviolet–visible spectroscopy. X-ray diffraction, transmission electron microscopy, and Fourier transform infrared spectroscopy (FTIR) were used to characterize the AgNPs. AgNPs exhibited a spherical morphology, uniform dispersion, and diameter ranging from ~8 to 9 nm. The FTIR data indicated that phytoconstituents, such as polyphenols, flavonoids, and terpenoids, could potentially serve as reducing and capping agents. The antibacterial activity of the synthesized AgNPs was assessed using broth dilution and agar well diffusion assays. The results demonstrate antibacterial effects against both Gram-positive and Gram-negative strains at low AgNP concentrations. The cytotoxicity of AgNPs was examined in vitro using the CCK-8 method, which showed that low concentrations of AgNPs are noncytotoxic to normal cells and promote cell growth. In conclusion, an environmentally friendly approach for synthesizing AgNPs from Gb leaves yielded antibacterial AgNPs with minimal toxicity, holding promise for future applications in the field of biomedicine.https://www.mdpi.com/1422-0067/25/3/1913green synthesis<i>Ginkgo biloba</i>silver nanomaterialsantimicrobial capacitycytotoxic activity |
| spellingShingle | Qi Ni Ting Zhu Wenjie Wang Dongdong Guo Yixiao Li Tianyu Chen Xiaojun Zhang Green Synthesis of Narrow-Size Silver Nanoparticles Using <i>Ginkgo biloba</i> Leaves: Condition Optimization, Characterization, and Antibacterial and Cytotoxic Activities International Journal of Molecular Sciences green synthesis <i>Ginkgo biloba</i> silver nanomaterials antimicrobial capacity cytotoxic activity |
| title | Green Synthesis of Narrow-Size Silver Nanoparticles Using <i>Ginkgo biloba</i> Leaves: Condition Optimization, Characterization, and Antibacterial and Cytotoxic Activities |
| title_full | Green Synthesis of Narrow-Size Silver Nanoparticles Using <i>Ginkgo biloba</i> Leaves: Condition Optimization, Characterization, and Antibacterial and Cytotoxic Activities |
| title_fullStr | Green Synthesis of Narrow-Size Silver Nanoparticles Using <i>Ginkgo biloba</i> Leaves: Condition Optimization, Characterization, and Antibacterial and Cytotoxic Activities |
| title_full_unstemmed | Green Synthesis of Narrow-Size Silver Nanoparticles Using <i>Ginkgo biloba</i> Leaves: Condition Optimization, Characterization, and Antibacterial and Cytotoxic Activities |
| title_short | Green Synthesis of Narrow-Size Silver Nanoparticles Using <i>Ginkgo biloba</i> Leaves: Condition Optimization, Characterization, and Antibacterial and Cytotoxic Activities |
| title_sort | green synthesis of narrow size silver nanoparticles using i ginkgo biloba i leaves condition optimization characterization and antibacterial and cytotoxic activities |
| topic | green synthesis <i>Ginkgo biloba</i> silver nanomaterials antimicrobial capacity cytotoxic activity |
| url | https://www.mdpi.com/1422-0067/25/3/1913 |
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