Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture
This present work describes the application of liquid chromatograpy-solid phase extraction-nuclear magnetic resonance spectroscopy to analyse Alternaria alternata crude extracts. Altenusin (1), alternariol (2), 3'-hydroxyalternariol monomethyl ether (3), and alternariol monomethyl ether (4)...
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-02-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/18/3/2528 |
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| author | Edson Rodrigues-Filho Cristina Daolio Bernd Schneider Gezimar D. de Souza Axel Mithöfer |
| author_facet | Edson Rodrigues-Filho Cristina Daolio Bernd Schneider Gezimar D. de Souza Axel Mithöfer |
| author_sort | Edson Rodrigues-Filho |
| collection | DOAJ |
| description | This present work describes the application of liquid chromatograpy-solid phase extraction-nuclear magnetic resonance spectroscopy to analyse Alternaria alternata crude extracts. Altenusin (1), alternariol (2), 3'-hydroxyalternariol monomethyl ether (3), and alternariol monomethyl ether (4), were separated and identified. High-resolution mass spectrometry confirmed the proposed structures. The cytotoxic effects of these compounds towards plants were determined using soybean (Glycine max) cell cultures as a model. EC50 values which range from 0.11 (±0.02) to 4.69 (±0.47) μM showed the high cytotoxicity of these compounds. |
| first_indexed | 2024-12-13T05:47:21Z |
| format | Article |
| id | doaj.art-5e22420e941e4b379a0d0359bb927c52 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-13T05:47:21Z |
| publishDate | 2013-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-5e22420e941e4b379a0d0359bb927c522022-12-21T23:57:38ZengMDPI AGMolecules1420-30492013-02-011832528253810.3390/molecules18032528Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension CultureEdson Rodrigues-FilhoCristina DaolioBernd SchneiderGezimar D. de SouzaAxel MithöferThis present work describes the application of liquid chromatograpy-solid phase extraction-nuclear magnetic resonance spectroscopy to analyse Alternaria alternata crude extracts. Altenusin (1), alternariol (2), 3'-hydroxyalternariol monomethyl ether (3), and alternariol monomethyl ether (4), were separated and identified. High-resolution mass spectrometry confirmed the proposed structures. The cytotoxic effects of these compounds towards plants were determined using soybean (Glycine max) cell cultures as a model. EC50 values which range from 0.11 (±0.02) to 4.69 (±0.47) μM showed the high cytotoxicity of these compounds.http://www.mdpi.com/1420-3049/18/3/2528LC-NMRLC-SPE-NMRmass spectrometryalternariolAlternariasoybeanGlycine maxcell culturescytotoxicity |
| spellingShingle | Edson Rodrigues-Filho Cristina Daolio Bernd Schneider Gezimar D. de Souza Axel Mithöfer Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture Molecules LC-NMR LC-SPE-NMR mass spectrometry alternariol Alternaria soybean Glycine max cell cultures cytotoxicity |
| title | Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture |
| title_full | Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture |
| title_fullStr | Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture |
| title_full_unstemmed | Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture |
| title_short | Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture |
| title_sort | identification of alternaria alternata mycotoxins by lc spe nmr and their cytotoxic effects to soybean glycine max cell suspension culture |
| topic | LC-NMR LC-SPE-NMR mass spectrometry alternariol Alternaria soybean Glycine max cell cultures cytotoxicity |
| url | http://www.mdpi.com/1420-3049/18/3/2528 |
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