Expression of adiponectin in the subchondral bone of lumbar facet joints with different degrees of degeneration
Abstract Background Osteoarthritis research has been most commonly performed in the setting of the articular cartilage of the knee. To the best of our knowledge, no studies have evaluated the role of adiponectin in osteoarthritis of the lumbar facet joint (FJOA). Therefore, in this study, we explore...
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| Language: | English |
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BMC
2017-11-01
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| Series: | BMC Musculoskeletal Disorders |
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| Online Access: | http://link.springer.com/article/10.1186/s12891-017-1786-8 |
| _version_ | 1818386074719748096 |
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| author | Qi Lai Yuan Liu Leitao Huang Xuqiang Liu Xionglong Yu Qiang Wang Runsheng Guo Jianghao Zhu Hanxiong Cheng Min Dai Bin Zhang |
| author_facet | Qi Lai Yuan Liu Leitao Huang Xuqiang Liu Xionglong Yu Qiang Wang Runsheng Guo Jianghao Zhu Hanxiong Cheng Min Dai Bin Zhang |
| author_sort | Qi Lai |
| collection | DOAJ |
| description | Abstract Background Osteoarthritis research has been most commonly performed in the setting of the articular cartilage of the knee. To the best of our knowledge, no studies have evaluated the role of adiponectin in osteoarthritis of the lumbar facet joint (FJOA). Therefore, in this study, we explored whether adiponectin was expressed in the lumbar facet joints and evaluated the role of adiponectin in FJOA. Methods We enrolled patients who underwent lumbar computed tomography (CT) and magnetic resonance imaging (MRI) at the Orthopedic Department of the First Affiliated Hospital of Nanchang from May 2015 to June 2016. Lumbar facet joints were obtained from 135 patients at the time of lumbar fusion surgery and divided into three groups according to the Weishaupt grade. Cytokine levels in the subchondral bones were evaluated by enzyme-linked immunosorbent assays (ELISAs), and adiponectin levels were determined by immunohistochemistry, western blotting, and quantitative polymerase chain reaction (qPCR). Results By ELISA, adiponectin levels were examined in the subchondral bone for lumbar facet joint, and adiponectin was found to be negatively correlated with BMI in 52 patients (p < 0.001, r = −0.861). By immunohistochemistry analysis, adiponectin was found to be expressed in the subchondral bone of the lumbar facet, whereas the cartilage area was negative for adiponectin expression. Immunostaining intensity and area was related to the degeneration of the lumbar facet joint, and, in our research, considerably decreased staining intensity and area were observed in more severely degenerated lumbar facet joints. Furthermore, the expression of adiponectin was also reduced in degenerated lumbar facet joints, and the level of decline corresponded to degeneration detected by western blotting and qPCR analysis (n = 27, p < 0.0001). Conclusions Adiponectin expression was observed in the subchondral bone of the lumbar facet joint and decreased as the degree of degeneration increased. Thus, the results of this study provide new insights into the relationship between adiponectin and osteoarthritis. |
| first_indexed | 2024-12-14T03:48:16Z |
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| id | doaj.art-5e283074638d4ab1b6792dd922787874 |
| institution | Directory Open Access Journal |
| issn | 1471-2474 |
| language | English |
| last_indexed | 2024-12-14T03:48:16Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
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| series | BMC Musculoskeletal Disorders |
| spelling | doaj.art-5e283074638d4ab1b6792dd9227878742022-12-21T23:18:17ZengBMCBMC Musculoskeletal Disorders1471-24742017-11-011811810.1186/s12891-017-1786-8Expression of adiponectin in the subchondral bone of lumbar facet joints with different degrees of degenerationQi Lai0Yuan Liu1Leitao Huang2Xuqiang Liu3Xionglong Yu4Qiang Wang5Runsheng Guo6Jianghao Zhu7Hanxiong Cheng8Min Dai9Bin Zhang10Department of Orthopedics, The First Affiliated Hospital of Nanchang UniversityDepartment of Orthopedics, The First Affiliated Hospital of Nanchang UniversityDepartment of Orthopedics, The First Affiliated Hospital of Nanchang UniversityDepartment of Orthopedics, The First Affiliated Hospital of Nanchang UniversityDepartment of Orthopedics, The First Affiliated Hospital of Nanchang UniversityDepartment of Orthopedics, The First Affiliated Hospital of Nanchang UniversityDepartment of Orthopedics, The First Affiliated Hospital of Nanchang UniversityDepartment of Orthopedics, The First Affiliated Hospital of Nanchang UniversityDepartment of Orthopedics, The First Affiliated Hospital of Nanchang UniversityDepartment of Orthopedics, The First Affiliated Hospital of Nanchang UniversityDepartment of Orthopedics, The First Affiliated Hospital of Nanchang UniversityAbstract Background Osteoarthritis research has been most commonly performed in the setting of the articular cartilage of the knee. To the best of our knowledge, no studies have evaluated the role of adiponectin in osteoarthritis of the lumbar facet joint (FJOA). Therefore, in this study, we explored whether adiponectin was expressed in the lumbar facet joints and evaluated the role of adiponectin in FJOA. Methods We enrolled patients who underwent lumbar computed tomography (CT) and magnetic resonance imaging (MRI) at the Orthopedic Department of the First Affiliated Hospital of Nanchang from May 2015 to June 2016. Lumbar facet joints were obtained from 135 patients at the time of lumbar fusion surgery and divided into three groups according to the Weishaupt grade. Cytokine levels in the subchondral bones were evaluated by enzyme-linked immunosorbent assays (ELISAs), and adiponectin levels were determined by immunohistochemistry, western blotting, and quantitative polymerase chain reaction (qPCR). Results By ELISA, adiponectin levels were examined in the subchondral bone for lumbar facet joint, and adiponectin was found to be negatively correlated with BMI in 52 patients (p < 0.001, r = −0.861). By immunohistochemistry analysis, adiponectin was found to be expressed in the subchondral bone of the lumbar facet, whereas the cartilage area was negative for adiponectin expression. Immunostaining intensity and area was related to the degeneration of the lumbar facet joint, and, in our research, considerably decreased staining intensity and area were observed in more severely degenerated lumbar facet joints. Furthermore, the expression of adiponectin was also reduced in degenerated lumbar facet joints, and the level of decline corresponded to degeneration detected by western blotting and qPCR analysis (n = 27, p < 0.0001). Conclusions Adiponectin expression was observed in the subchondral bone of the lumbar facet joint and decreased as the degree of degeneration increased. Thus, the results of this study provide new insights into the relationship between adiponectin and osteoarthritis.http://link.springer.com/article/10.1186/s12891-017-1786-8AdiponectinFacet jointOsteoarthritisSubchondral boneCartilageBone degeneration |
| spellingShingle | Qi Lai Yuan Liu Leitao Huang Xuqiang Liu Xionglong Yu Qiang Wang Runsheng Guo Jianghao Zhu Hanxiong Cheng Min Dai Bin Zhang Expression of adiponectin in the subchondral bone of lumbar facet joints with different degrees of degeneration BMC Musculoskeletal Disorders Adiponectin Facet joint Osteoarthritis Subchondral bone Cartilage Bone degeneration |
| title | Expression of adiponectin in the subchondral bone of lumbar facet joints with different degrees of degeneration |
| title_full | Expression of adiponectin in the subchondral bone of lumbar facet joints with different degrees of degeneration |
| title_fullStr | Expression of adiponectin in the subchondral bone of lumbar facet joints with different degrees of degeneration |
| title_full_unstemmed | Expression of adiponectin in the subchondral bone of lumbar facet joints with different degrees of degeneration |
| title_short | Expression of adiponectin in the subchondral bone of lumbar facet joints with different degrees of degeneration |
| title_sort | expression of adiponectin in the subchondral bone of lumbar facet joints with different degrees of degeneration |
| topic | Adiponectin Facet joint Osteoarthritis Subchondral bone Cartilage Bone degeneration |
| url | http://link.springer.com/article/10.1186/s12891-017-1786-8 |
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