Molecular Characterization of <i>bHLH</i> Transcription Factor Family in Rose (<i>Rosa chinensis</i> Jacq.) under <i>Botrytis cinerea</i> Infection
Rose (<i>Rosa chinensis</i> Jacq.) is an important economic ornamental crop and its yield is affected by different biotic and abiotic stresses. Among the biotic stresses, the gray mold disease caused by <i>Botrytis cinerea</i> is a serious threat to rose production. The <i...
| Autori principali: | , , , , , , |
|---|---|
| Natura: | Articolo |
| Lingua: | English |
| Pubblicazione: |
MDPI AG
2022-10-01
|
| Serie: | Horticulturae |
| Soggetti: | |
| Accesso online: | https://www.mdpi.com/2311-7524/8/11/989 |
| _version_ | 1827646497367261184 |
|---|---|
| author | Ikram Ullah Wenbin Yuan Muhammad Uzair Sisi Li Obaid Ur Rehman Satyabrata Nanda Hongzhi Wu |
| author_facet | Ikram Ullah Wenbin Yuan Muhammad Uzair Sisi Li Obaid Ur Rehman Satyabrata Nanda Hongzhi Wu |
| author_sort | Ikram Ullah |
| collection | DOAJ |
| description | Rose (<i>Rosa chinensis</i> Jacq.) is an important economic ornamental crop and its yield is affected by different biotic and abiotic stresses. Among the biotic stresses, the gray mold disease caused by <i>Botrytis cinerea</i> is a serious threat to rose production. The <i>basic helix-loop-helix</i> (<i>bHLH</i>) is a large transcription factor family involved in several vital plant physiological processes, including growth, development, and stress response. However, no substantial reports exist on <i>bHLH</i> genes in rose. Here, the genome-wide identification, characterization, and expression analysis of the rose <i>bHLH</i> (<i>RcbHLH</i>) genes was carried out. In total, 100 <i>RcbHLH</i>s were identified in the rose genome and mapped onto different rose chromosomes. The gene duplication analysis revealed both tandem and segmental duplications in <i>RcbHLH</i>s. The RcbHLHs among other plant bHLHs were divided into 21 sub-groups on the phylogenetic tree. Additionally, prediction of the different <i>cis</i>-regulatory elements and the gene ontology of the identified <i>RcbHLH</i>s indicated their possible functions in rose plants. The expression analysis of selected <i>RcbHLH</i>s genes in two contrasting rose varieties (A29 = Black Baccara and XS = Sweet Avalanche) under <i>B. cinerea</i> infection provided insights into the involvement of <i>RcbHLH</i>s in rose–<i>B</i>. <i>cinerea</i> interactions. Moreover, this study provided details on the <i>bHLH</i> family genes in rose and their potential roles in rose defense against <i>B.</i> <i>cinerea</i> infection. |
| first_indexed | 2024-03-09T19:02:30Z |
| format | Article |
| id | doaj.art-5e2910e321454bc7a67edfc9d87d6a3d |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T19:02:30Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-5e2910e321454bc7a67edfc9d87d6a3d2023-11-24T04:52:25ZengMDPI AGHorticulturae2311-75242022-10-0181198910.3390/horticulturae8110989Molecular Characterization of <i>bHLH</i> Transcription Factor Family in Rose (<i>Rosa chinensis</i> Jacq.) under <i>Botrytis cinerea</i> InfectionIkram Ullah0Wenbin Yuan1Muhammad Uzair2Sisi Li3Obaid Ur Rehman4Satyabrata Nanda5Hongzhi Wu6College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, ChinaNational Institute for Genomics and Advanced Biotechnology (NIGAB), Park Road, Islamabad 45500, PakistanCollege of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, ChinaNational Institute for Genomics and Advanced Biotechnology (NIGAB), Park Road, Islamabad 45500, PakistanMS Swaminathan School of Agriculture, Centurion University of Technology and Management, Paralakhemundi 761211, IndiaCollege of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, ChinaRose (<i>Rosa chinensis</i> Jacq.) is an important economic ornamental crop and its yield is affected by different biotic and abiotic stresses. Among the biotic stresses, the gray mold disease caused by <i>Botrytis cinerea</i> is a serious threat to rose production. The <i>basic helix-loop-helix</i> (<i>bHLH</i>) is a large transcription factor family involved in several vital plant physiological processes, including growth, development, and stress response. However, no substantial reports exist on <i>bHLH</i> genes in rose. Here, the genome-wide identification, characterization, and expression analysis of the rose <i>bHLH</i> (<i>RcbHLH</i>) genes was carried out. In total, 100 <i>RcbHLH</i>s were identified in the rose genome and mapped onto different rose chromosomes. The gene duplication analysis revealed both tandem and segmental duplications in <i>RcbHLH</i>s. The RcbHLHs among other plant bHLHs were divided into 21 sub-groups on the phylogenetic tree. Additionally, prediction of the different <i>cis</i>-regulatory elements and the gene ontology of the identified <i>RcbHLH</i>s indicated their possible functions in rose plants. The expression analysis of selected <i>RcbHLH</i>s genes in two contrasting rose varieties (A29 = Black Baccara and XS = Sweet Avalanche) under <i>B. cinerea</i> infection provided insights into the involvement of <i>RcbHLH</i>s in rose–<i>B</i>. <i>cinerea</i> interactions. Moreover, this study provided details on the <i>bHLH</i> family genes in rose and their potential roles in rose defense against <i>B.</i> <i>cinerea</i> infection.https://www.mdpi.com/2311-7524/8/11/989rosebiotic stress<i>Botrytis cinerea</i>basic helix-loop-helix (bHLH)expression analysis |
| spellingShingle | Ikram Ullah Wenbin Yuan Muhammad Uzair Sisi Li Obaid Ur Rehman Satyabrata Nanda Hongzhi Wu Molecular Characterization of <i>bHLH</i> Transcription Factor Family in Rose (<i>Rosa chinensis</i> Jacq.) under <i>Botrytis cinerea</i> Infection Horticulturae rose biotic stress <i>Botrytis cinerea</i> basic helix-loop-helix (bHLH) expression analysis |
| title | Molecular Characterization of <i>bHLH</i> Transcription Factor Family in Rose (<i>Rosa chinensis</i> Jacq.) under <i>Botrytis cinerea</i> Infection |
| title_full | Molecular Characterization of <i>bHLH</i> Transcription Factor Family in Rose (<i>Rosa chinensis</i> Jacq.) under <i>Botrytis cinerea</i> Infection |
| title_fullStr | Molecular Characterization of <i>bHLH</i> Transcription Factor Family in Rose (<i>Rosa chinensis</i> Jacq.) under <i>Botrytis cinerea</i> Infection |
| title_full_unstemmed | Molecular Characterization of <i>bHLH</i> Transcription Factor Family in Rose (<i>Rosa chinensis</i> Jacq.) under <i>Botrytis cinerea</i> Infection |
| title_short | Molecular Characterization of <i>bHLH</i> Transcription Factor Family in Rose (<i>Rosa chinensis</i> Jacq.) under <i>Botrytis cinerea</i> Infection |
| title_sort | molecular characterization of i bhlh i transcription factor family in rose i rosa chinensis i jacq under i botrytis cinerea i infection |
| topic | rose biotic stress <i>Botrytis cinerea</i> basic helix-loop-helix (bHLH) expression analysis |
| url | https://www.mdpi.com/2311-7524/8/11/989 |
| work_keys_str_mv | AT ikramullah molecularcharacterizationofibhlhitranscriptionfactorfamilyinroseirosachinensisijacqunderibotrytiscinereaiinfection AT wenbinyuan molecularcharacterizationofibhlhitranscriptionfactorfamilyinroseirosachinensisijacqunderibotrytiscinereaiinfection AT muhammaduzair molecularcharacterizationofibhlhitranscriptionfactorfamilyinroseirosachinensisijacqunderibotrytiscinereaiinfection AT sisili molecularcharacterizationofibhlhitranscriptionfactorfamilyinroseirosachinensisijacqunderibotrytiscinereaiinfection AT obaidurrehman molecularcharacterizationofibhlhitranscriptionfactorfamilyinroseirosachinensisijacqunderibotrytiscinereaiinfection AT satyabratananda molecularcharacterizationofibhlhitranscriptionfactorfamilyinroseirosachinensisijacqunderibotrytiscinereaiinfection AT hongzhiwu molecularcharacterizationofibhlhitranscriptionfactorfamilyinroseirosachinensisijacqunderibotrytiscinereaiinfection |