Emerging technologies for engineering of extracellular vesicles
Extracellular vesicles (EVs) are lipid-bilayer membrane-enclosed vesicles that are secreted by all cell types. Natural EVs contain biological information such as proteins, nucleic acids, and lipids from their parent cells. Therefore, EVs have been extensively studied as diagnostic biomarkers and the...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1298746/full |
| _version_ | 1827763896108187648 |
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| author | Xin Zhou Xin Zhou Qing Huang Yang Jiang Huijing Tang Huijing Tang Luhan Zhang Danyang Li Yunsheng Xu Yunsheng Xu |
| author_facet | Xin Zhou Xin Zhou Qing Huang Yang Jiang Huijing Tang Huijing Tang Luhan Zhang Danyang Li Yunsheng Xu Yunsheng Xu |
| author_sort | Xin Zhou |
| collection | DOAJ |
| description | Extracellular vesicles (EVs) are lipid-bilayer membrane-enclosed vesicles that are secreted by all cell types. Natural EVs contain biological information such as proteins, nucleic acids, and lipids from their parent cells. Therefore, EVs have been extensively studied as diagnostic biomarkers and therapeutic tools under normal and pathological conditions. However, some drawbacks, including low yield, poor therapeutic effects, lack of imaging, and targeting capacity of natural EVs, still need to be improved. Emerging engineering technologies have rendered EVs new properties or functionalities that broadened their applications in the biomedical field. Herein, in this review, we gave a brief overview of advanced strategies for EV engineering. We focused on pre-treatment of parent cells to regulate their released EVs. Meanwhile, we summarized and discussed the direct modification of EVs to achieve drug loading, imaging, and targeting functionalities for downstream applications. |
| first_indexed | 2024-03-11T10:57:13Z |
| format | Article |
| id | doaj.art-5e2eb092d7df438582666bde59444458 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-11T10:57:13Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-5e2eb092d7df438582666bde594444582023-11-13T04:27:56ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-11-011110.3389/fbioe.2023.12987461298746Emerging technologies for engineering of extracellular vesiclesXin Zhou0Xin Zhou1Qing Huang2Yang Jiang3Huijing Tang4Huijing Tang5Luhan Zhang6Danyang Li7Yunsheng Xu8Yunsheng Xu9Research Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaDepartment of Dermatovenereology, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaResearch Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaResearch Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaResearch Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaDepartment of Dermatovenereology, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaResearch Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaResearch Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaResearch Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaDepartment of Dermatovenereology, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaExtracellular vesicles (EVs) are lipid-bilayer membrane-enclosed vesicles that are secreted by all cell types. Natural EVs contain biological information such as proteins, nucleic acids, and lipids from their parent cells. Therefore, EVs have been extensively studied as diagnostic biomarkers and therapeutic tools under normal and pathological conditions. However, some drawbacks, including low yield, poor therapeutic effects, lack of imaging, and targeting capacity of natural EVs, still need to be improved. Emerging engineering technologies have rendered EVs new properties or functionalities that broadened their applications in the biomedical field. Herein, in this review, we gave a brief overview of advanced strategies for EV engineering. We focused on pre-treatment of parent cells to regulate their released EVs. Meanwhile, we summarized and discussed the direct modification of EVs to achieve drug loading, imaging, and targeting functionalities for downstream applications.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1298746/fullextracellular vesicleengineering strategypre-treatmentparent celldirect modification |
| spellingShingle | Xin Zhou Xin Zhou Qing Huang Yang Jiang Huijing Tang Huijing Tang Luhan Zhang Danyang Li Yunsheng Xu Yunsheng Xu Emerging technologies for engineering of extracellular vesicles Frontiers in Bioengineering and Biotechnology extracellular vesicle engineering strategy pre-treatment parent cell direct modification |
| title | Emerging technologies for engineering of extracellular vesicles |
| title_full | Emerging technologies for engineering of extracellular vesicles |
| title_fullStr | Emerging technologies for engineering of extracellular vesicles |
| title_full_unstemmed | Emerging technologies for engineering of extracellular vesicles |
| title_short | Emerging technologies for engineering of extracellular vesicles |
| title_sort | emerging technologies for engineering of extracellular vesicles |
| topic | extracellular vesicle engineering strategy pre-treatment parent cell direct modification |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1298746/full |
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