Comparison of Fibrin and PLGA/fibrin Scaffolds for Chondrogenesis of Human Adipose Derived Stem Cells by Icariin
Background: Recently, cartilage tissue engineering is the best candidate for regeneration of cartilage defects. We evaluated the potential of fibrin and PLGA/fibrin scaffolds in providing a suitable environment for growth and chondrogenic differentiation of human adipose derived stem cells (hADSCs)...
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Kerman University of Medical Sciences
2020-01-01
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| Series: | Journal of Kerman University of Medical Sciences |
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| Online Access: | https://jkmu.kmu.ac.ir/article_89592_6882d2fd389534bffbd5387464c27a9b.pdf |
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| author | Batool Hashemibeni Mohammad Mardani Maryam Bahrami Ali Valiani Mohsen Setayesh Mehr Majid Pourentezari |
| author_facet | Batool Hashemibeni Mohammad Mardani Maryam Bahrami Ali Valiani Mohsen Setayesh Mehr Majid Pourentezari |
| author_sort | Batool Hashemibeni |
| collection | DOAJ |
| description | Background: Recently, cartilage tissue engineering is the best candidate for regeneration of cartilage defects. We evaluated the potential of fibrin and PLGA/fibrin scaffolds in providing a suitable environment for growth and chondrogenic differentiation of human adipose derived stem cells (hADSCs) in the presence of icariin. Method: The Three-dimensional (3-D) PLGA scaffold was prepared using the solvent casting/salt leaching technique and the hybrid scaffold was fabricated by fibrin. hADSCs were isolated from human adipose tissue. 3-D PLGA/fibrin scaffolds were seeded with cultured hADSCs and analyzed 14 days later, Monolayer culture was used for the control group. The viabilities of cells in different groups were assessed by MTT. The expression of chondrogenic related genes, hypertrophic marker and Fibrotic marker were quantified by RT-PCR. Results:MTT results show that viability in the control group was significantly higher than those in the Fibrin and PLGA/Fibrin groups. Also viability in the PLGA/Fibrin group affected by icariin was higher than that in Fibrin group. The results of the real-time PCR showed that SOX9, Agg, Coll 2, and Coll 1 gene expression in the fibrin and PLGA/fibrin groups were significantly higher than those in the control group. Coll 10 gene expression in the fibrin group was higher in comparison to the control group but not significantly. type SOX9, Coll 2 and Coll 1 gene expression in the fibrin group was significantly lower compared to the PLGA/fibrin group. Conclusions: The study reveals that the corporation of PLGA with fibrin is an effective way to potentially enhance articular cartilage regeneration of hADSCs in the presence of icariin. |
| first_indexed | 2024-03-13T02:09:04Z |
| format | Article |
| id | doaj.art-5e2fd84f7b4943d2800c0b953db1de7b |
| institution | Directory Open Access Journal |
| issn | 2008-2843 |
| language | English |
| last_indexed | 2024-03-13T02:09:04Z |
| publishDate | 2020-01-01 |
| publisher | Kerman University of Medical Sciences |
| record_format | Article |
| series | Journal of Kerman University of Medical Sciences |
| spelling | doaj.art-5e2fd84f7b4943d2800c0b953db1de7b2023-07-01T05:14:57ZengKerman University of Medical SciencesJournal of Kerman University of Medical Sciences2008-28432020-01-01271142310.22062/jkmu.2020.8959289592Comparison of Fibrin and PLGA/fibrin Scaffolds for Chondrogenesis of Human Adipose Derived Stem Cells by IcariinBatool Hashemibeni0Mohammad Mardani1Maryam Bahrami2Ali Valiani3Mohsen Setayesh Mehr4Majid Pourentezari5Professor, Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, IranProfessor, Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, IranPh.D. Candidate, Anatomy Department, Medical Faculty, Shaheed Beheshti University of Medical Sciences, Tehran, IranAssociate Professor, Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Nanotechnology and Tissue Engineering Group, Department of Advanced Medical Technology, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor. Department of Anatomical Sciences and Molecular Biology, Shahid Sadoughi University of Medical Sciences, Yazd, IranBackground: Recently, cartilage tissue engineering is the best candidate for regeneration of cartilage defects. We evaluated the potential of fibrin and PLGA/fibrin scaffolds in providing a suitable environment for growth and chondrogenic differentiation of human adipose derived stem cells (hADSCs) in the presence of icariin. Method: The Three-dimensional (3-D) PLGA scaffold was prepared using the solvent casting/salt leaching technique and the hybrid scaffold was fabricated by fibrin. hADSCs were isolated from human adipose tissue. 3-D PLGA/fibrin scaffolds were seeded with cultured hADSCs and analyzed 14 days later, Monolayer culture was used for the control group. The viabilities of cells in different groups were assessed by MTT. The expression of chondrogenic related genes, hypertrophic marker and Fibrotic marker were quantified by RT-PCR. Results:MTT results show that viability in the control group was significantly higher than those in the Fibrin and PLGA/Fibrin groups. Also viability in the PLGA/Fibrin group affected by icariin was higher than that in Fibrin group. The results of the real-time PCR showed that SOX9, Agg, Coll 2, and Coll 1 gene expression in the fibrin and PLGA/fibrin groups were significantly higher than those in the control group. Coll 10 gene expression in the fibrin group was higher in comparison to the control group but not significantly. type SOX9, Coll 2 and Coll 1 gene expression in the fibrin group was significantly lower compared to the PLGA/fibrin group. Conclusions: The study reveals that the corporation of PLGA with fibrin is an effective way to potentially enhance articular cartilage regeneration of hADSCs in the presence of icariin.https://jkmu.kmu.ac.ir/article_89592_6882d2fd389534bffbd5387464c27a9b.pdficariinchondrogenesishuman adipose derived stem cell (hadsc)fibrinpoly (lactic-co-glycolic) acid/fibrinscaffold |
| spellingShingle | Batool Hashemibeni Mohammad Mardani Maryam Bahrami Ali Valiani Mohsen Setayesh Mehr Majid Pourentezari Comparison of Fibrin and PLGA/fibrin Scaffolds for Chondrogenesis of Human Adipose Derived Stem Cells by Icariin Journal of Kerman University of Medical Sciences icariin chondrogenesis human adipose derived stem cell (hadsc) fibrin poly (lactic-co-glycolic) acid/fibrin scaffold |
| title | Comparison of Fibrin and PLGA/fibrin Scaffolds for Chondrogenesis of Human Adipose Derived Stem Cells by Icariin |
| title_full | Comparison of Fibrin and PLGA/fibrin Scaffolds for Chondrogenesis of Human Adipose Derived Stem Cells by Icariin |
| title_fullStr | Comparison of Fibrin and PLGA/fibrin Scaffolds for Chondrogenesis of Human Adipose Derived Stem Cells by Icariin |
| title_full_unstemmed | Comparison of Fibrin and PLGA/fibrin Scaffolds for Chondrogenesis of Human Adipose Derived Stem Cells by Icariin |
| title_short | Comparison of Fibrin and PLGA/fibrin Scaffolds for Chondrogenesis of Human Adipose Derived Stem Cells by Icariin |
| title_sort | comparison of fibrin and plga fibrin scaffolds for chondrogenesis of human adipose derived stem cells by icariin |
| topic | icariin chondrogenesis human adipose derived stem cell (hadsc) fibrin poly (lactic-co-glycolic) acid/fibrin scaffold |
| url | https://jkmu.kmu.ac.ir/article_89592_6882d2fd389534bffbd5387464c27a9b.pdf |
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