Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial Cells
Porcine epidemic diarrhea virus (PEDV), which is the causative agent of porcine epidemic diarrhea in China and other countries, is responsible for serious economic losses in the pork industry. Inactivated PEDV vaccine plays a key role in controlling the prevalence of PEDV. However, consistently low...
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| Format: | Article |
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MDPI AG
2017-01-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/9/1/19 |
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| author | Wen Shi Shuo Jia Haiyuan Zhao Jiyuan Yin Xiaona Wang Meiling Yu Sunting Ma Yang Wu Ying Chen Wenlu Fan Yigang Xu Yijing Li |
| author_facet | Wen Shi Shuo Jia Haiyuan Zhao Jiyuan Yin Xiaona Wang Meiling Yu Sunting Ma Yang Wu Ying Chen Wenlu Fan Yigang Xu Yijing Li |
| author_sort | Wen Shi |
| collection | DOAJ |
| description | Porcine epidemic diarrhea virus (PEDV), which is the causative agent of porcine epidemic diarrhea in China and other countries, is responsible for serious economic losses in the pork industry. Inactivated PEDV vaccine plays a key role in controlling the prevalence of PEDV. However, consistently low viral titers are obtained during the propagation of PEDV in vitro; this represents a challenge to molecular analyses of the virus and vaccine development. In this study, we successfully isolated a PEDV isolate (strain NJ) from clinical samples collected during a recent outbreak of diarrhea in piglets in China, using porcine intestinal epithelial cells (IEC). We found that the isolate was better adapted to growth in IECs than in Vero cells, and the titer of the IEC cultures was 104.5 TCID50/0.1 mL at passage 45. Mutations in the S protein increased with the viral passage and the mutations tended towards attenuation. Viral challenge showed that the survival of IEC-adapted cultures was higher at the 45th passage than at the 5th passage. The use of IECs to isolate and propagate PEDV provides an effective approach for laboratory-based diagnosis of PEDV, as well as studies of the epidemiological characteristics and molecular biology of this virus. |
| first_indexed | 2024-12-22T07:02:45Z |
| format | Article |
| id | doaj.art-5e311f38ab634b349d039dc29c431221 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-22T07:02:45Z |
| publishDate | 2017-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-5e311f38ab634b349d039dc29c4312212022-12-21T18:34:45ZengMDPI AGViruses1999-49152017-01-01911910.3390/v9010019v9010019Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial CellsWen Shi0Shuo Jia1Haiyuan Zhao2Jiyuan Yin3Xiaona Wang4Meiling Yu5Sunting Ma6Yang Wu7Ying Chen8Wenlu Fan9Yigang Xu10Yijing Li11College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaPorcine epidemic diarrhea virus (PEDV), which is the causative agent of porcine epidemic diarrhea in China and other countries, is responsible for serious economic losses in the pork industry. Inactivated PEDV vaccine plays a key role in controlling the prevalence of PEDV. However, consistently low viral titers are obtained during the propagation of PEDV in vitro; this represents a challenge to molecular analyses of the virus and vaccine development. In this study, we successfully isolated a PEDV isolate (strain NJ) from clinical samples collected during a recent outbreak of diarrhea in piglets in China, using porcine intestinal epithelial cells (IEC). We found that the isolate was better adapted to growth in IECs than in Vero cells, and the titer of the IEC cultures was 104.5 TCID50/0.1 mL at passage 45. Mutations in the S protein increased with the viral passage and the mutations tended towards attenuation. Viral challenge showed that the survival of IEC-adapted cultures was higher at the 45th passage than at the 5th passage. The use of IECs to isolate and propagate PEDV provides an effective approach for laboratory-based diagnosis of PEDV, as well as studies of the epidemiological characteristics and molecular biology of this virus.http://www.mdpi.com/1999-4915/9/1/19porcine epidemic diarrhea virus (PEDV) NJ strainporcine intestinal epithelial cellsisolation and identification |
| spellingShingle | Wen Shi Shuo Jia Haiyuan Zhao Jiyuan Yin Xiaona Wang Meiling Yu Sunting Ma Yang Wu Ying Chen Wenlu Fan Yigang Xu Yijing Li Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial Cells Viruses porcine epidemic diarrhea virus (PEDV) NJ strain porcine intestinal epithelial cells isolation and identification |
| title | Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial Cells |
| title_full | Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial Cells |
| title_fullStr | Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial Cells |
| title_full_unstemmed | Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial Cells |
| title_short | Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial Cells |
| title_sort | novel approach for isolation and identification of porcine epidemic diarrhea virus pedv strain nj using porcine intestinal epithelial cells |
| topic | porcine epidemic diarrhea virus (PEDV) NJ strain porcine intestinal epithelial cells isolation and identification |
| url | http://www.mdpi.com/1999-4915/9/1/19 |
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