Metabarcoding Reveals Temporal Patterns of Community Composition and Realized Thermal Niches of <i>Thalassiosira</i> Spp. (Bacillariophyceae) from the Narragansett Bay Long-Term Plankton Time Series
Diatoms generate nearly half of marine primary production and are comprised of a diverse array of species that are often morphologically cryptic or difficult to identify using light microscopy. Here, species composition and realized thermal niches of species in the diatom genus <i>Thalassiosir...
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| Format: | Article |
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MDPI AG
2020-01-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/9/1/19 |
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| author | Tatiana A. Rynearson Sarah A. Flickinger Diana N. Fontaine |
| author_facet | Tatiana A. Rynearson Sarah A. Flickinger Diana N. Fontaine |
| author_sort | Tatiana A. Rynearson |
| collection | DOAJ |
| description | Diatoms generate nearly half of marine primary production and are comprised of a diverse array of species that are often morphologically cryptic or difficult to identify using light microscopy. Here, species composition and realized thermal niches of species in the diatom genus <i>Thalassiosira</i> were examined at the site of the Narragansett Bay (NBay) Long-Term Plankton Time Series using a combination of light microscopy (LM), high-throughput sequencing (HTS) of the 18S rDNA V4 region and historical records. <i>Thalassiosira</i> species were identified over 6 years using a combination of LM and DNA sequences. Sixteen <i>Thalassiosira</i> taxa were identified using HTS: nine were newly identified in NBay. Several newly identified species have small cell diameters and are difficult to identify using LM. However, they appeared frequently and thus may play a significant ecological role in NBay, particularly since their realized niches suggest they are eurythermal and able to tolerate the >25 °C temperature range of NBay. Four distinct species assemblages that grouped by season were best explained by surface water temperature. When compared to historical records, we found that the cold-water species <i>Thalassiosira nordenskioeldii</i> has decreased in persistence over time, suggesting that increasing surface water temperature has influenced the ecology of phytoplankton in NBay. |
| first_indexed | 2024-03-12T19:14:00Z |
| format | Article |
| id | doaj.art-5e326d9b09774108abc394c690cc2573 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-12T19:14:00Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-5e326d9b09774108abc394c690cc25732023-08-02T05:38:15ZengMDPI AGBiology2079-77372020-01-01911910.3390/biology9010019biology9010019Metabarcoding Reveals Temporal Patterns of Community Composition and Realized Thermal Niches of <i>Thalassiosira</i> Spp. (Bacillariophyceae) from the Narragansett Bay Long-Term Plankton Time SeriesTatiana A. Rynearson0Sarah A. Flickinger1Diana N. Fontaine2Graduate School of Oceanography, University of Rhode Island, South Ferry Road, Narragansett, RI 02882, USAGraduate School of Oceanography, University of Rhode Island, South Ferry Road, Narragansett, RI 02882, USAGraduate School of Oceanography, University of Rhode Island, South Ferry Road, Narragansett, RI 02882, USADiatoms generate nearly half of marine primary production and are comprised of a diverse array of species that are often morphologically cryptic or difficult to identify using light microscopy. Here, species composition and realized thermal niches of species in the diatom genus <i>Thalassiosira</i> were examined at the site of the Narragansett Bay (NBay) Long-Term Plankton Time Series using a combination of light microscopy (LM), high-throughput sequencing (HTS) of the 18S rDNA V4 region and historical records. <i>Thalassiosira</i> species were identified over 6 years using a combination of LM and DNA sequences. Sixteen <i>Thalassiosira</i> taxa were identified using HTS: nine were newly identified in NBay. Several newly identified species have small cell diameters and are difficult to identify using LM. However, they appeared frequently and thus may play a significant ecological role in NBay, particularly since their realized niches suggest they are eurythermal and able to tolerate the >25 °C temperature range of NBay. Four distinct species assemblages that grouped by season were best explained by surface water temperature. When compared to historical records, we found that the cold-water species <i>Thalassiosira nordenskioeldii</i> has decreased in persistence over time, suggesting that increasing surface water temperature has influenced the ecology of phytoplankton in NBay.https://www.mdpi.com/2079-7737/9/1/19community compositiondiatomdiversityhigh throughput sequencingmolecular ecologyphytoplanktonmetabarcoding |
| spellingShingle | Tatiana A. Rynearson Sarah A. Flickinger Diana N. Fontaine Metabarcoding Reveals Temporal Patterns of Community Composition and Realized Thermal Niches of <i>Thalassiosira</i> Spp. (Bacillariophyceae) from the Narragansett Bay Long-Term Plankton Time Series Biology community composition diatom diversity high throughput sequencing molecular ecology phytoplankton metabarcoding |
| title | Metabarcoding Reveals Temporal Patterns of Community Composition and Realized Thermal Niches of <i>Thalassiosira</i> Spp. (Bacillariophyceae) from the Narragansett Bay Long-Term Plankton Time Series |
| title_full | Metabarcoding Reveals Temporal Patterns of Community Composition and Realized Thermal Niches of <i>Thalassiosira</i> Spp. (Bacillariophyceae) from the Narragansett Bay Long-Term Plankton Time Series |
| title_fullStr | Metabarcoding Reveals Temporal Patterns of Community Composition and Realized Thermal Niches of <i>Thalassiosira</i> Spp. (Bacillariophyceae) from the Narragansett Bay Long-Term Plankton Time Series |
| title_full_unstemmed | Metabarcoding Reveals Temporal Patterns of Community Composition and Realized Thermal Niches of <i>Thalassiosira</i> Spp. (Bacillariophyceae) from the Narragansett Bay Long-Term Plankton Time Series |
| title_short | Metabarcoding Reveals Temporal Patterns of Community Composition and Realized Thermal Niches of <i>Thalassiosira</i> Spp. (Bacillariophyceae) from the Narragansett Bay Long-Term Plankton Time Series |
| title_sort | metabarcoding reveals temporal patterns of community composition and realized thermal niches of i thalassiosira i spp bacillariophyceae from the narragansett bay long term plankton time series |
| topic | community composition diatom diversity high throughput sequencing molecular ecology phytoplankton metabarcoding |
| url | https://www.mdpi.com/2079-7737/9/1/19 |
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