Improved γ-linolenic acid production in Mucor circinelloides by homologous overexpressing of delta-12 and delta-6 desaturases
Abstract Background γ-Linolenic acid (GLA) is important because of its nutritional value and medicinal applications. Although the biosynthetic pathways of some plant and microbial GLA have been deciphered, current understanding of the correlation between desaturases and GLA synthesis in oleaginous f...
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| Format: | Article |
| Language: | English |
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BMC
2017-06-01
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| Series: | Microbial Cell Factories |
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| Online Access: | http://link.springer.com/article/10.1186/s12934-017-0723-8 |
| _version_ | 1818436340826505216 |
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| author | Yao Zhang Xiao Luan Huaiyuan Zhang Victoriano Garre Yuanda Song Colin Ratledge |
| author_facet | Yao Zhang Xiao Luan Huaiyuan Zhang Victoriano Garre Yuanda Song Colin Ratledge |
| author_sort | Yao Zhang |
| collection | DOAJ |
| description | Abstract Background γ-Linolenic acid (GLA) is important because of its nutritional value and medicinal applications. Although the biosynthetic pathways of some plant and microbial GLA have been deciphered, current understanding of the correlation between desaturases and GLA synthesis in oleaginous fungi is incomplete. In previous work, we found that a large amount of oleic acid (OA) had not been converted to linoleic acid (LA) or GLA in Mucor circinelloides CBS 277.49, which may be due to inadequate activities of the delta-12 or delta-6 desaturases, and thus leading to the accumulation of OA and LA. Thus, it is necessary to explore the main contributing factor during the process of GLA biosynthesis in M. circinelloides. Results To enhance GLA production in M. circinelloides, homologous overexpression of delta-12 and two delta-6 desaturases (named delta-6-1 and delta-6-2, respectively) were analyzed. When delta-6 desaturase were overexpressed in M. circinelloides, up to 43% GLA was produced in the total fatty acids, and the yield of GLA reached 180 mg/l, which were, respectively, 38 and 33% higher than the control strain. Conclusion These findings revealed that delta-6 desaturase (especially for delta-6-1 desaturase) plays an important role in GLA synthesis by M. circinelloides. The strain overexpressing delta-6-1 desaturase may have potential application in microbial GLA production. |
| first_indexed | 2024-12-14T17:07:14Z |
| format | Article |
| id | doaj.art-5e35508de47946329d5e277f7fd0bf10 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-14T17:07:14Z |
| publishDate | 2017-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-5e35508de47946329d5e277f7fd0bf102022-12-21T22:53:41ZengBMCMicrobial Cell Factories1475-28592017-06-011611910.1186/s12934-017-0723-8Improved γ-linolenic acid production in Mucor circinelloides by homologous overexpressing of delta-12 and delta-6 desaturasesYao Zhang0Xiao Luan1Huaiyuan Zhang2Victoriano Garre3Yuanda Song4Colin Ratledge5Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Key Laboratory of Shandong Provincial Universities for Technologies in Functional Agricultural Products, Shandong University of TechnologySchool of Food Science and Technolgy, Jiangnan UniversityColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Key Laboratory of Shandong Provincial Universities for Technologies in Functional Agricultural Products, Shandong University of TechnologyDepartamento de Genéticay Microbiologia (Unidad ASOCIADA al IQFR-CSIC), Facultad de Biologi, Universidad de MurciaColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Key Laboratory of Shandong Provincial Universities for Technologies in Functional Agricultural Products, Shandong University of TechnologyDepartment of Biological Sciences, University of HullAbstract Background γ-Linolenic acid (GLA) is important because of its nutritional value and medicinal applications. Although the biosynthetic pathways of some plant and microbial GLA have been deciphered, current understanding of the correlation between desaturases and GLA synthesis in oleaginous fungi is incomplete. In previous work, we found that a large amount of oleic acid (OA) had not been converted to linoleic acid (LA) or GLA in Mucor circinelloides CBS 277.49, which may be due to inadequate activities of the delta-12 or delta-6 desaturases, and thus leading to the accumulation of OA and LA. Thus, it is necessary to explore the main contributing factor during the process of GLA biosynthesis in M. circinelloides. Results To enhance GLA production in M. circinelloides, homologous overexpression of delta-12 and two delta-6 desaturases (named delta-6-1 and delta-6-2, respectively) were analyzed. When delta-6 desaturase were overexpressed in M. circinelloides, up to 43% GLA was produced in the total fatty acids, and the yield of GLA reached 180 mg/l, which were, respectively, 38 and 33% higher than the control strain. Conclusion These findings revealed that delta-6 desaturase (especially for delta-6-1 desaturase) plays an important role in GLA synthesis by M. circinelloides. The strain overexpressing delta-6-1 desaturase may have potential application in microbial GLA production.http://link.springer.com/article/10.1186/s12934-017-0723-8Delta-6 desaturaseDelta-12 desaturaseGLA productionHomologous overexpressionMucor circinelloides |
| spellingShingle | Yao Zhang Xiao Luan Huaiyuan Zhang Victoriano Garre Yuanda Song Colin Ratledge Improved γ-linolenic acid production in Mucor circinelloides by homologous overexpressing of delta-12 and delta-6 desaturases Microbial Cell Factories Delta-6 desaturase Delta-12 desaturase GLA production Homologous overexpression Mucor circinelloides |
| title | Improved γ-linolenic acid production in Mucor circinelloides by homologous overexpressing of delta-12 and delta-6 desaturases |
| title_full | Improved γ-linolenic acid production in Mucor circinelloides by homologous overexpressing of delta-12 and delta-6 desaturases |
| title_fullStr | Improved γ-linolenic acid production in Mucor circinelloides by homologous overexpressing of delta-12 and delta-6 desaturases |
| title_full_unstemmed | Improved γ-linolenic acid production in Mucor circinelloides by homologous overexpressing of delta-12 and delta-6 desaturases |
| title_short | Improved γ-linolenic acid production in Mucor circinelloides by homologous overexpressing of delta-12 and delta-6 desaturases |
| title_sort | improved γ linolenic acid production in mucor circinelloides by homologous overexpressing of delta 12 and delta 6 desaturases |
| topic | Delta-6 desaturase Delta-12 desaturase GLA production Homologous overexpression Mucor circinelloides |
| url | http://link.springer.com/article/10.1186/s12934-017-0723-8 |
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