The effect of serum-free media on the metabolic yields and growth rates of C2C12 cells in the context of cultivated meat production
Successful commercialization of cultivated meat products as alternative sources of dietary protein for a growing global population will require development of efficient and inexpensive serum-free cell culture media. It is known that serum-free media elicit dissimilar cell growth behavior compared to...
| Main Authors: | , , , , , , |
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| Format: | Article |
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Elsevier
2023-06-01
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| Series: | Future Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666833523000126 |
| _version_ | 1797800973975945216 |
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| author | Edward N. O'Neill Milla Neffling Nick Randall Grace Kwong Joshua Ansel Keith Baar David E. Block |
| author_facet | Edward N. O'Neill Milla Neffling Nick Randall Grace Kwong Joshua Ansel Keith Baar David E. Block |
| author_sort | Edward N. O'Neill |
| collection | DOAJ |
| description | Successful commercialization of cultivated meat products as alternative sources of dietary protein for a growing global population will require development of efficient and inexpensive serum-free cell culture media. It is known that serum-free media elicit dissimilar cell growth behavior compared to serum-based media, but data comprehensively exploring the effects of serum-free culture on the nutrient requirements of cultivated meat-relevant cell types have yet to be reported. We performed spent media analysis of C2C12 cells growing in Essential 8 serum-free media and in conventional serum-containing media. Data indicated that although the cell growth rates were similar in the two media over seven days, there were significant differences in the utilization rates of some key nutrients such as glucose, glutamine, glycine, and cystine. We extrapolated from the data that roughly 250–275 g of amino acids and 1100–1500 g of glucose would be required to produce 1 kg of C2C12 cells, though these requirements may change when using more optimized cells, media, and feed strategies. Our results highlight the influence of serum and serum replacements on cell metabolism, and indicate that previous data from metabolic studies performed using serum-containing media may not directly translate to serum-free systems. |
| first_indexed | 2024-03-13T04:43:22Z |
| format | Article |
| id | doaj.art-5e363e8010284b30b1ce7c443e6e7ea8 |
| institution | Directory Open Access Journal |
| issn | 2666-8335 |
| language | English |
| last_indexed | 2024-03-13T04:43:22Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Future Foods |
| spelling | doaj.art-5e363e8010284b30b1ce7c443e6e7ea82023-06-19T04:29:57ZengElsevierFuture Foods2666-83352023-06-017100226The effect of serum-free media on the metabolic yields and growth rates of C2C12 cells in the context of cultivated meat productionEdward N. O'Neill0Milla Neffling1Nick Randall2Grace Kwong3Joshua Ansel4Keith Baar5David E. Block6Department of Food Science and Technology, University of California, Davis, Davis, CA, United States; Department of Viticulture and Enology, University of California, Davis, 595 Hilgard Lane, Davis, CA 95616, United States908 Devices, Boston, MA, United States908 Devices, Boston, MA, United StatesDepartment of Chemical Engineering, University of California, Davis, Davis, CA, United StatesDepartment of Food Science and Technology, University of California, Davis, Davis, CA, United StatesDepartment of Neurobiology, Physiology, and Behavior, University of California, Davis, One Shields Ave, Davis, CA 95616, United States; Department of Physiology and Membrane Biology, University of California, Davis, Davis, CA, United States; Corresponding author at: Department of Neurobiology, Physiology, and Behavior, University of California, Davis, One Shields Ave, Davis, CA 95616, United States.Department of Viticulture and Enology, University of California, Davis, 595 Hilgard Lane, Davis, CA 95616, United States; Department of Chemical Engineering, University of California, Davis, Davis, CA, United States; Corresponding author at: Department of Viticulture and Enology, University of California, Davis, Davis, CA 95616, United States.Successful commercialization of cultivated meat products as alternative sources of dietary protein for a growing global population will require development of efficient and inexpensive serum-free cell culture media. It is known that serum-free media elicit dissimilar cell growth behavior compared to serum-based media, but data comprehensively exploring the effects of serum-free culture on the nutrient requirements of cultivated meat-relevant cell types have yet to be reported. We performed spent media analysis of C2C12 cells growing in Essential 8 serum-free media and in conventional serum-containing media. Data indicated that although the cell growth rates were similar in the two media over seven days, there were significant differences in the utilization rates of some key nutrients such as glucose, glutamine, glycine, and cystine. We extrapolated from the data that roughly 250–275 g of amino acids and 1100–1500 g of glucose would be required to produce 1 kg of C2C12 cells, though these requirements may change when using more optimized cells, media, and feed strategies. Our results highlight the influence of serum and serum replacements on cell metabolism, and indicate that previous data from metabolic studies performed using serum-containing media may not directly translate to serum-free systems.http://www.sciencedirect.com/science/article/pii/S2666833523000126Alternative proteinsCultured meatSerum-free mediaMedia optimizationSpent media analysisEssential 8 |
| spellingShingle | Edward N. O'Neill Milla Neffling Nick Randall Grace Kwong Joshua Ansel Keith Baar David E. Block The effect of serum-free media on the metabolic yields and growth rates of C2C12 cells in the context of cultivated meat production Future Foods Alternative proteins Cultured meat Serum-free media Media optimization Spent media analysis Essential 8 |
| title | The effect of serum-free media on the metabolic yields and growth rates of C2C12 cells in the context of cultivated meat production |
| title_full | The effect of serum-free media on the metabolic yields and growth rates of C2C12 cells in the context of cultivated meat production |
| title_fullStr | The effect of serum-free media on the metabolic yields and growth rates of C2C12 cells in the context of cultivated meat production |
| title_full_unstemmed | The effect of serum-free media on the metabolic yields and growth rates of C2C12 cells in the context of cultivated meat production |
| title_short | The effect of serum-free media on the metabolic yields and growth rates of C2C12 cells in the context of cultivated meat production |
| title_sort | effect of serum free media on the metabolic yields and growth rates of c2c12 cells in the context of cultivated meat production |
| topic | Alternative proteins Cultured meat Serum-free media Media optimization Spent media analysis Essential 8 |
| url | http://www.sciencedirect.com/science/article/pii/S2666833523000126 |
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