The Expression and Role Analysis of Methylation-Regulated Differentially Expressed Gene UBE2C in Pan-Cancer, Especially for HGSOC
High-grade serous ovarian cancer (HGSOC) is the most fatal gynecological malignant tumor. DNA methylation is associated with the occurrence and development of a variety of tumor types, including HGSOC. However, the signatures regarding DNA methylation changes for HGSOC diagnosis and prognosis are le...
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MDPI AG
2022-06-01
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| Series: | Cancers |
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| author | Jiajia Li Yating Sun Xiuling Zhi Qin Li Liangqing Yao Mo Chen |
| author_facet | Jiajia Li Yating Sun Xiuling Zhi Qin Li Liangqing Yao Mo Chen |
| author_sort | Jiajia Li |
| collection | DOAJ |
| description | High-grade serous ovarian cancer (HGSOC) is the most fatal gynecological malignant tumor. DNA methylation is associated with the occurrence and development of a variety of tumor types, including HGSOC. However, the signatures regarding DNA methylation changes for HGSOC diagnosis and prognosis are less explored. Here, we screened differentially methylated genes and differentially expressed genes in HGSOC through the GEO database. We identified that UBE2C was hypomethylation and overexpression in ovarian cancer, which was associated with more advanced cancer stages and poor prognoses. Additionally, the pan-cancer analysis showed that UBE2C was overexpressed and hypomethylation in almost all cancer types and was related to poor prognoses for various cancers. Next, we established a risk or prognosis model related to UBE2C methylation sites and screened out the three sites (cg03969725, cg02838589, and cg00242976). Furthermore, we experimentally validated the overexpression of UBE2C in HGSOC clinical samples and ovarian cell lines using quantitative real-time PCR, Western blot, and immunohistochemistry. Importantly, we discovered that ovarian cancer cell lines had lower DNA methylation levels of UBE2C than IOSE-80 cells (normal ovarian epithelial cell line) by bisulfite sequencing PCR. Consistently, treatment with 5-Azacytidine (a methylation inhibitor) was able to restore the expression of UBE2C. Taken together, our study may help us to understand the underlying molecular mechanism of UBE2C in pan-cancer tumorigenesis; it may be a useful biomarker for diagnosis, treatment, and monitoring, not only of ovarian cancer but a variety of cancers. |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T22:03:28Z |
| publishDate | 2022-06-01 |
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| spelling | doaj.art-5e3849ca08de4a4cb92d2334614e0d3e2023-11-23T19:44:48ZengMDPI AGCancers2072-66942022-06-011413312110.3390/cancers14133121The Expression and Role Analysis of Methylation-Regulated Differentially Expressed Gene UBE2C in Pan-Cancer, Especially for HGSOCJiajia Li0Yating Sun1Xiuling Zhi2Qin Li3Liangqing Yao4Mo Chen5Department of Gynecology Oncology, Obstetrics & Gynecology Hospital, Fudan University, Shanghai 200011, ChinaDepartment of Gynecology Oncology, Obstetrics & Gynecology Hospital, Fudan University, Shanghai 200011, ChinaDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, ChinaDepartment of Gynecology Oncology, Obstetrics & Gynecology Hospital, Fudan University, Shanghai 200011, ChinaDepartment of Gynecology Oncology, Obstetrics & Gynecology Hospital, Fudan University, Shanghai 200011, ChinaDepartment of Gynecology Oncology, Obstetrics & Gynecology Hospital, Fudan University, Shanghai 200011, ChinaHigh-grade serous ovarian cancer (HGSOC) is the most fatal gynecological malignant tumor. DNA methylation is associated with the occurrence and development of a variety of tumor types, including HGSOC. However, the signatures regarding DNA methylation changes for HGSOC diagnosis and prognosis are less explored. Here, we screened differentially methylated genes and differentially expressed genes in HGSOC through the GEO database. We identified that UBE2C was hypomethylation and overexpression in ovarian cancer, which was associated with more advanced cancer stages and poor prognoses. Additionally, the pan-cancer analysis showed that UBE2C was overexpressed and hypomethylation in almost all cancer types and was related to poor prognoses for various cancers. Next, we established a risk or prognosis model related to UBE2C methylation sites and screened out the three sites (cg03969725, cg02838589, and cg00242976). Furthermore, we experimentally validated the overexpression of UBE2C in HGSOC clinical samples and ovarian cell lines using quantitative real-time PCR, Western blot, and immunohistochemistry. Importantly, we discovered that ovarian cancer cell lines had lower DNA methylation levels of UBE2C than IOSE-80 cells (normal ovarian epithelial cell line) by bisulfite sequencing PCR. Consistently, treatment with 5-Azacytidine (a methylation inhibitor) was able to restore the expression of UBE2C. Taken together, our study may help us to understand the underlying molecular mechanism of UBE2C in pan-cancer tumorigenesis; it may be a useful biomarker for diagnosis, treatment, and monitoring, not only of ovarian cancer but a variety of cancers.https://www.mdpi.com/2072-6694/14/13/3121ovarian cancerDNA methylationUBE2Cbioinformatics |
| spellingShingle | Jiajia Li Yating Sun Xiuling Zhi Qin Li Liangqing Yao Mo Chen The Expression and Role Analysis of Methylation-Regulated Differentially Expressed Gene UBE2C in Pan-Cancer, Especially for HGSOC Cancers ovarian cancer DNA methylation UBE2C bioinformatics |
| title | The Expression and Role Analysis of Methylation-Regulated Differentially Expressed Gene UBE2C in Pan-Cancer, Especially for HGSOC |
| title_full | The Expression and Role Analysis of Methylation-Regulated Differentially Expressed Gene UBE2C in Pan-Cancer, Especially for HGSOC |
| title_fullStr | The Expression and Role Analysis of Methylation-Regulated Differentially Expressed Gene UBE2C in Pan-Cancer, Especially for HGSOC |
| title_full_unstemmed | The Expression and Role Analysis of Methylation-Regulated Differentially Expressed Gene UBE2C in Pan-Cancer, Especially for HGSOC |
| title_short | The Expression and Role Analysis of Methylation-Regulated Differentially Expressed Gene UBE2C in Pan-Cancer, Especially for HGSOC |
| title_sort | expression and role analysis of methylation regulated differentially expressed gene ube2c in pan cancer especially for hgsoc |
| topic | ovarian cancer DNA methylation UBE2C bioinformatics |
| url | https://www.mdpi.com/2072-6694/14/13/3121 |
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