Body Composition QTLs Identified in Intercross Populations Are Reproducible in Consomic Mouse Strains.
Genetic variation contributes to individual differences in obesity, but defining the exact relationships between naturally occurring genotypes and their effects on fatness remains elusive. As a step toward positional cloning of previously identified body composition quantitative trait loci (QTLs) fr...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0141494 |
| _version_ | 1819032657158209536 |
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| author | Cailu Lin Brad D Fesi Michael Marquis Natalia P Bosak Maria L Theodorides Mauricio Avigdor Amanda H McDaniel Fujiko F Duke Anna Lysenko Amin Khoshnevisan Brian R Gantick Charles J Arayata Theodore M Nelson Alexander A Bachmanov Danielle R Reed |
| author_facet | Cailu Lin Brad D Fesi Michael Marquis Natalia P Bosak Maria L Theodorides Mauricio Avigdor Amanda H McDaniel Fujiko F Duke Anna Lysenko Amin Khoshnevisan Brian R Gantick Charles J Arayata Theodore M Nelson Alexander A Bachmanov Danielle R Reed |
| author_sort | Cailu Lin |
| collection | DOAJ |
| description | Genetic variation contributes to individual differences in obesity, but defining the exact relationships between naturally occurring genotypes and their effects on fatness remains elusive. As a step toward positional cloning of previously identified body composition quantitative trait loci (QTLs) from F2 crosses of mice from the C57BL/6ByJ and 129P3/J inbred strains, we sought to recapture them on a homogenous genetic background of consomic (chromosome substitution) strains. Male and female mice from reciprocal consomic strains originating from the C57BL/6ByJ and 129P3/J strains were bred and measured for body weight, length, and adiposity. Chromosomes 2, 7, and 9 were selected for substitution because previous F2 intercross studies revealed body composition QTLs on these chromosomes. We considered a QTL confirmed if one or both sexes of one or both reciprocal consomic strains differed significantly from the host strain in the expected direction after correction for multiple testing. Using these criteria, we confirmed two of two QTLs for body weight (Bwq5-6), three of three QTLs for body length (Bdln3-5), and three of three QTLs for adiposity (Adip20, Adip26 and Adip27). Overall, this study shows that despite the biological complexity of body size and composition, most QTLs for these traits are preserved when transferred to consomic strains; in addition, studying reciprocal consomic strains of both sexes is useful in assessing the robustness of a particular QTL. |
| first_indexed | 2024-12-21T07:05:25Z |
| format | Article |
| id | doaj.art-5e399d678a1e41b589aeb2c3351270e3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T07:05:25Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5e399d678a1e41b589aeb2c3351270e32022-12-21T19:12:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011011e014149410.1371/journal.pone.0141494Body Composition QTLs Identified in Intercross Populations Are Reproducible in Consomic Mouse Strains.Cailu LinBrad D FesiMichael MarquisNatalia P BosakMaria L TheodoridesMauricio AvigdorAmanda H McDanielFujiko F DukeAnna LysenkoAmin KhoshnevisanBrian R GantickCharles J ArayataTheodore M NelsonAlexander A BachmanovDanielle R ReedGenetic variation contributes to individual differences in obesity, but defining the exact relationships between naturally occurring genotypes and their effects on fatness remains elusive. As a step toward positional cloning of previously identified body composition quantitative trait loci (QTLs) from F2 crosses of mice from the C57BL/6ByJ and 129P3/J inbred strains, we sought to recapture them on a homogenous genetic background of consomic (chromosome substitution) strains. Male and female mice from reciprocal consomic strains originating from the C57BL/6ByJ and 129P3/J strains were bred and measured for body weight, length, and adiposity. Chromosomes 2, 7, and 9 were selected for substitution because previous F2 intercross studies revealed body composition QTLs on these chromosomes. We considered a QTL confirmed if one or both sexes of one or both reciprocal consomic strains differed significantly from the host strain in the expected direction after correction for multiple testing. Using these criteria, we confirmed two of two QTLs for body weight (Bwq5-6), three of three QTLs for body length (Bdln3-5), and three of three QTLs for adiposity (Adip20, Adip26 and Adip27). Overall, this study shows that despite the biological complexity of body size and composition, most QTLs for these traits are preserved when transferred to consomic strains; in addition, studying reciprocal consomic strains of both sexes is useful in assessing the robustness of a particular QTL.https://doi.org/10.1371/journal.pone.0141494 |
| spellingShingle | Cailu Lin Brad D Fesi Michael Marquis Natalia P Bosak Maria L Theodorides Mauricio Avigdor Amanda H McDaniel Fujiko F Duke Anna Lysenko Amin Khoshnevisan Brian R Gantick Charles J Arayata Theodore M Nelson Alexander A Bachmanov Danielle R Reed Body Composition QTLs Identified in Intercross Populations Are Reproducible in Consomic Mouse Strains. PLoS ONE |
| title | Body Composition QTLs Identified in Intercross Populations Are Reproducible in Consomic Mouse Strains. |
| title_full | Body Composition QTLs Identified in Intercross Populations Are Reproducible in Consomic Mouse Strains. |
| title_fullStr | Body Composition QTLs Identified in Intercross Populations Are Reproducible in Consomic Mouse Strains. |
| title_full_unstemmed | Body Composition QTLs Identified in Intercross Populations Are Reproducible in Consomic Mouse Strains. |
| title_short | Body Composition QTLs Identified in Intercross Populations Are Reproducible in Consomic Mouse Strains. |
| title_sort | body composition qtls identified in intercross populations are reproducible in consomic mouse strains |
| url | https://doi.org/10.1371/journal.pone.0141494 |
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