Heavy metals mitigation and growth promoting effect of endophytic Agrococcus terreus (MW 979614) in maize plants under zinc and nickel contaminated soil
IntroductionHeavy metals such as iron, copper, manganese, cobalt, silver, zinc, nickel, and arsenic have accumulated in soils for a long time due to the dumping of industrial waste and sewage. Various techniques have been adapted to overcome metal toxicity in agricultural land but utilizing a biolog...
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1255921/full |
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| author | Asim Shahzad Asim Shahzad Anam Siddique Shazia Ferdous Muhammad Ahmar Amin Mingzhou Qin Uzma Aslam Muhammad Naeem Tasmia Bashir Abdul Shakoor |
| author_facet | Asim Shahzad Asim Shahzad Anam Siddique Shazia Ferdous Muhammad Ahmar Amin Mingzhou Qin Uzma Aslam Muhammad Naeem Tasmia Bashir Abdul Shakoor |
| author_sort | Asim Shahzad |
| collection | DOAJ |
| description | IntroductionHeavy metals such as iron, copper, manganese, cobalt, silver, zinc, nickel, and arsenic have accumulated in soils for a long time due to the dumping of industrial waste and sewage. Various techniques have been adapted to overcome metal toxicity in agricultural land but utilizing a biological application using potential microorganisms in heavy metals contaminated soil may be a successful approach to decontaminate heavy metals soil. Therefore, the current study aimed to isolate endophytic bacteria from a medicinal plant (Viburnum grandiflorum) and to investigate the growth-promoting and heavy metal detoxification potential of the isolated endophytic bacteria Agrococus tereus (GenBank accession number MW 979614) under nickel and zinc contamination.MethodsZinc sulfate and nickel sulfate solutions were prepared at the rate of 100 mg/kg and 50 mg/kg in sterilized distilled water. The experiment was conducted using a completely random design (CRD) with three replicates for each treatment.Results and DiscussionInoculation of seeds with A. tereus significantly increased the plant growth, nutrient uptake, and defense system. Treatment T4 (inoculated seeds), T5 (inoculated seeds + Zn100 mg/kg), and T6 (inoculated seeds + Ni 100 mg/kg) were effective, but T5 (inoculated seeds + Zn100 mg/kg) was the most pronounced and increased shoot length, root length, leaf width, plant height, fresh weight, moisture content, and proline by 49%, 38%, 89%, 31%, 113%, and 146%, respectively. Moreover the antioxidant enzymes peroxidase and super oxidase dismutase were accelerated by 211 and 68% in contaminated soil when plants were inoculated by A. tereus respectively. Similarly the inoculation of A. tereus also enhanced maize plants’ absorption of Cu, Mn, Ni, Na, Cr, Fe, Ca, Mg, and K significantly. Results of the findings concluded that 100 mg/kg of Zn and Ni were toxic to maize growth, but seed inoculation with A. tereus helped the plants significantly in reducing zinc and nickel stress. The A. tereus strain may be employed as a potential strain for the detoxification of heavy metals |
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| id | doaj.art-5e3a8ac3d76d44c8b10cc3b212690788 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-11T10:53:05Z |
| publishDate | 2023-11-01 |
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| spelling | doaj.art-5e3a8ac3d76d44c8b10cc3b2126907882023-11-13T12:37:06ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-11-011410.3389/fmicb.2023.12559211255921Heavy metals mitigation and growth promoting effect of endophytic Agrococcus terreus (MW 979614) in maize plants under zinc and nickel contaminated soilAsim Shahzad0Asim Shahzad1Anam Siddique2Shazia Ferdous3Muhammad Ahmar Amin4Mingzhou Qin5Uzma Aslam6Muhammad Naeem7Tasmia Bashir8Abdul Shakoor9The College of Geography and Environment, Henan University, Kaifeng, ChinaDepartment of Botany, Mohi-Ud-Din Islamic University, AJ&K, PakistanDepartment of Botany, Mohi-Ud-Din Islamic University, AJ&K, PakistanDepartment of Botany, Mohi-Ud-Din Islamic University, AJ&K, PakistanRawalpindi Women University, Rawalpindi, PakistanThe College of Geography and Environment, Henan University, Kaifeng, ChinaDepartment of Botany, Mohi-Ud-Din Islamic University, AJ&K, PakistanDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Botany, Rawalpindi Women University Rawalpindi, Rawalpindi, PakistanThe College of Geography and Environment, Henan University, Kaifeng, ChinaIntroductionHeavy metals such as iron, copper, manganese, cobalt, silver, zinc, nickel, and arsenic have accumulated in soils for a long time due to the dumping of industrial waste and sewage. Various techniques have been adapted to overcome metal toxicity in agricultural land but utilizing a biological application using potential microorganisms in heavy metals contaminated soil may be a successful approach to decontaminate heavy metals soil. Therefore, the current study aimed to isolate endophytic bacteria from a medicinal plant (Viburnum grandiflorum) and to investigate the growth-promoting and heavy metal detoxification potential of the isolated endophytic bacteria Agrococus tereus (GenBank accession number MW 979614) under nickel and zinc contamination.MethodsZinc sulfate and nickel sulfate solutions were prepared at the rate of 100 mg/kg and 50 mg/kg in sterilized distilled water. The experiment was conducted using a completely random design (CRD) with three replicates for each treatment.Results and DiscussionInoculation of seeds with A. tereus significantly increased the plant growth, nutrient uptake, and defense system. Treatment T4 (inoculated seeds), T5 (inoculated seeds + Zn100 mg/kg), and T6 (inoculated seeds + Ni 100 mg/kg) were effective, but T5 (inoculated seeds + Zn100 mg/kg) was the most pronounced and increased shoot length, root length, leaf width, plant height, fresh weight, moisture content, and proline by 49%, 38%, 89%, 31%, 113%, and 146%, respectively. Moreover the antioxidant enzymes peroxidase and super oxidase dismutase were accelerated by 211 and 68% in contaminated soil when plants were inoculated by A. tereus respectively. Similarly the inoculation of A. tereus also enhanced maize plants’ absorption of Cu, Mn, Ni, Na, Cr, Fe, Ca, Mg, and K significantly. Results of the findings concluded that 100 mg/kg of Zn and Ni were toxic to maize growth, but seed inoculation with A. tereus helped the plants significantly in reducing zinc and nickel stress. The A. tereus strain may be employed as a potential strain for the detoxification of heavy metalshttps://www.frontiersin.org/articles/10.3389/fmicb.2023.1255921/fullendophytic bacterialAgrococus terreusZea maysheavy metals stresszinc contamination nickel contaminationantioxidant enzymes |
| spellingShingle | Asim Shahzad Asim Shahzad Anam Siddique Shazia Ferdous Muhammad Ahmar Amin Mingzhou Qin Uzma Aslam Muhammad Naeem Tasmia Bashir Abdul Shakoor Heavy metals mitigation and growth promoting effect of endophytic Agrococcus terreus (MW 979614) in maize plants under zinc and nickel contaminated soil Frontiers in Microbiology endophytic bacterial Agrococus terreus Zea mays heavy metals stress zinc contamination nickel contamination antioxidant enzymes |
| title | Heavy metals mitigation and growth promoting effect of endophytic Agrococcus terreus (MW 979614) in maize plants under zinc and nickel contaminated soil |
| title_full | Heavy metals mitigation and growth promoting effect of endophytic Agrococcus terreus (MW 979614) in maize plants under zinc and nickel contaminated soil |
| title_fullStr | Heavy metals mitigation and growth promoting effect of endophytic Agrococcus terreus (MW 979614) in maize plants under zinc and nickel contaminated soil |
| title_full_unstemmed | Heavy metals mitigation and growth promoting effect of endophytic Agrococcus terreus (MW 979614) in maize plants under zinc and nickel contaminated soil |
| title_short | Heavy metals mitigation and growth promoting effect of endophytic Agrococcus terreus (MW 979614) in maize plants under zinc and nickel contaminated soil |
| title_sort | heavy metals mitigation and growth promoting effect of endophytic agrococcus terreus mw 979614 in maize plants under zinc and nickel contaminated soil |
| topic | endophytic bacterial Agrococus terreus Zea mays heavy metals stress zinc contamination nickel contamination antioxidant enzymes |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1255921/full |
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