Effect of Ganoderma lucidum polysaccharides as immunostimulants against Vibrio harveyi in pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus)
Immunostimulants are becoming one of the most effective models for healthy and sustainable development against vibriosis, which is a serious threat to the global aquaculture industry. This study evaluated the effects of different concentrations of Ganoderma lucidum polysaccharides (GLPs) on the immu...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.968838/full |
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| author | Yilin Zhang Jian Zhong Yucong Huang Yucong Huang Jichang Jian Jichang Jian Shuanghu Cai Shuanghu Cai |
| author_facet | Yilin Zhang Jian Zhong Yucong Huang Yucong Huang Jichang Jian Jichang Jian Shuanghu Cai Shuanghu Cai |
| author_sort | Yilin Zhang |
| collection | DOAJ |
| description | Immunostimulants are becoming one of the most effective models for healthy and sustainable development against vibriosis, which is a serious threat to the global aquaculture industry. This study evaluated the effects of different concentrations of Ganoderma lucidum polysaccharides (GLPs) on the immunomodulation and disease resistance of pearl gentian groupers. The relative percent survival of the pearl gentian groupers challenged with Vibrio harveyi ZJ0603 by intraperitoneal injection reached 53.3, 60, 70, and 73.3% in the GLPs2, GLPs4, GLPs6, and GLPs8 groups, respectively, which are significantly higher than that of 33.3% in the control group. Meanwhile, the expression levels of immune-relative genes, including IgM, MHC-Iα, MHC2, IL-1β, and TNF-α, were upregulated in the liver, spleen, head kidney, and thymus within 28 days after GLP injection. The total serum protein and the activities of catalase, superoxide dismutase, and lysozymes in serum were significantly upregulated in all GLP groups compared with those in the control. Moreover, the optimal immunity effect was observed with an injection of GLPs at concentrations of 6 or 8 mg/ml. The results demonstrate that GLPs were an effective immunostimulant, enhancing both specific and non-specific immunity as well as disease resistance in pearl gentian grouper. |
| first_indexed | 2024-12-10T12:58:18Z |
| format | Article |
| id | doaj.art-5e3d2d22d8aa45d89d8434694405db80 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-10T12:58:18Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-5e3d2d22d8aa45d89d8434694405db802022-12-22T01:48:01ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-09-01910.3389/fmars.2022.968838968838Effect of Ganoderma lucidum polysaccharides as immunostimulants against Vibrio harveyi in pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus)Yilin Zhang0Jian Zhong1Yucong Huang2Yucong Huang3Jichang Jian4Jichang Jian5Shuanghu Cai6Shuanghu Cai7Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture and Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Fisheries College of Guangdong Ocean University, Zhanjiang, ChinaZhanjiang Custom District, Zhanjiang Customs, Zhanjiang, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture and Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Fisheries College of Guangdong Ocean University, Zhanjiang, ChinaGuangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen Institute of Guangdong Ocean University, Shenzhen, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture and Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Fisheries College of Guangdong Ocean University, Zhanjiang, ChinaGuangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen Institute of Guangdong Ocean University, Shenzhen, ChinaGuangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture and Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Fisheries College of Guangdong Ocean University, Zhanjiang, ChinaGuangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen Institute of Guangdong Ocean University, Shenzhen, ChinaImmunostimulants are becoming one of the most effective models for healthy and sustainable development against vibriosis, which is a serious threat to the global aquaculture industry. This study evaluated the effects of different concentrations of Ganoderma lucidum polysaccharides (GLPs) on the immunomodulation and disease resistance of pearl gentian groupers. The relative percent survival of the pearl gentian groupers challenged with Vibrio harveyi ZJ0603 by intraperitoneal injection reached 53.3, 60, 70, and 73.3% in the GLPs2, GLPs4, GLPs6, and GLPs8 groups, respectively, which are significantly higher than that of 33.3% in the control group. Meanwhile, the expression levels of immune-relative genes, including IgM, MHC-Iα, MHC2, IL-1β, and TNF-α, were upregulated in the liver, spleen, head kidney, and thymus within 28 days after GLP injection. The total serum protein and the activities of catalase, superoxide dismutase, and lysozymes in serum were significantly upregulated in all GLP groups compared with those in the control. Moreover, the optimal immunity effect was observed with an injection of GLPs at concentrations of 6 or 8 mg/ml. The results demonstrate that GLPs were an effective immunostimulant, enhancing both specific and non-specific immunity as well as disease resistance in pearl gentian grouper.https://www.frontiersin.org/articles/10.3389/fmars.2022.968838/fullVibrio harveyiGanoderma lucidum polysaccharidesimmune enhancerpearl gentian grouperintraperitoneal injection |
| spellingShingle | Yilin Zhang Jian Zhong Yucong Huang Yucong Huang Jichang Jian Jichang Jian Shuanghu Cai Shuanghu Cai Effect of Ganoderma lucidum polysaccharides as immunostimulants against Vibrio harveyi in pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus) Frontiers in Marine Science Vibrio harveyi Ganoderma lucidum polysaccharides immune enhancer pearl gentian grouper intraperitoneal injection |
| title | Effect of Ganoderma lucidum polysaccharides as immunostimulants against Vibrio harveyi in pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus) |
| title_full | Effect of Ganoderma lucidum polysaccharides as immunostimulants against Vibrio harveyi in pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus) |
| title_fullStr | Effect of Ganoderma lucidum polysaccharides as immunostimulants against Vibrio harveyi in pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus) |
| title_full_unstemmed | Effect of Ganoderma lucidum polysaccharides as immunostimulants against Vibrio harveyi in pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus) |
| title_short | Effect of Ganoderma lucidum polysaccharides as immunostimulants against Vibrio harveyi in pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus) |
| title_sort | effect of ganoderma lucidum polysaccharides as immunostimulants against vibrio harveyi in pearl gentian grouper ♀epinephelus fuscoguttatus ♂epinephelus lanceolatus |
| topic | Vibrio harveyi Ganoderma lucidum polysaccharides immune enhancer pearl gentian grouper intraperitoneal injection |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.968838/full |
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