The Association between New World Alphasatellites and Bipartite Begomoviruses: Effects on Infection and Vector Transmission
Begomoviruses can be found in association with alphasatellites, which are capable of autonomous replication but are dependent on the helper begomovirus for systemic infection, encapsidation and vector transmission. Previous studies suggest that the presence of NW alphasatellites (genus <i>Clec...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/10/1244 |
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| author | Angélica M. Nogueira Monique B. Nascimento Tarsiane M. C. Barbosa Ayane F. F. Quadros João Paulo A. Gomes Anelise F. Orílio Danielle R. Barros Francisco Murilo Zerbini |
| author_facet | Angélica M. Nogueira Monique B. Nascimento Tarsiane M. C. Barbosa Ayane F. F. Quadros João Paulo A. Gomes Anelise F. Orílio Danielle R. Barros Francisco Murilo Zerbini |
| author_sort | Angélica M. Nogueira |
| collection | DOAJ |
| description | Begomoviruses can be found in association with alphasatellites, which are capable of autonomous replication but are dependent on the helper begomovirus for systemic infection, encapsidation and vector transmission. Previous studies suggest that the presence of NW alphasatellites (genus <i>Clecrusatellite</i>) is associated with more severe symptoms. To better understand this interaction, we investigated the effects of two alphasatellites on infectivity, symptom development, viral DNA accumulation and vector transmission of three begomoviruses in three hosts. In tomato and <i>Nicotiana benthamiana</i>, all combinations were infectious. In <i>Leonurus sibiricus</i>, only the ToYSV/ToYSA combination was infectious. The presence of EuYMA increased symptom severity of EuYMV and ToYSV in <i>N. benthamiana</i>, and the presence of ToYSA was associated with more severe symptoms of ToYSV in <i>N. benthamiana</i> and <i>L. sibiricus</i>. EuYMA increased the accumulation of ToYSV in <i>N. benthamiana</i> but reduced the accumulation of EuYMV in tomato and of ToSRV in <i>N. benthamiana</i>. The presence of ToYSA decreased the accumulation of ToYSV in <i>N. benthamiana</i> and <i>L. sibiricus</i>. ToYSA negatively affected transmission of ToSRV by <i>Bemisia tabaci</i> MEAM1. Together, our results indicate that NW alphasatellites can interact with different begomoviruses, increasing symptom severity and interfering in the transmission of the helper begomovirus. Understanding this interaction is important as it may affect the emergence of diseases caused by begomovirus–alphasatellite complexes in the field. |
| first_indexed | 2024-03-10T06:17:11Z |
| format | Article |
| id | doaj.art-5e3d987f33674bd09aa6cfad3fa5b010 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T06:17:11Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
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| series | Pathogens |
| spelling | doaj.art-5e3d987f33674bd09aa6cfad3fa5b0102023-11-22T19:32:53ZengMDPI AGPathogens2076-08172021-09-011010124410.3390/pathogens10101244The Association between New World Alphasatellites and Bipartite Begomoviruses: Effects on Infection and Vector TransmissionAngélica M. Nogueira0Monique B. Nascimento1Tarsiane M. C. Barbosa2Ayane F. F. Quadros3João Paulo A. Gomes4Anelise F. Orílio5Danielle R. Barros6Francisco Murilo Zerbini7Departamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa 36570-900, BrazilDepartamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa 36570-900, BrazilDepartamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa 36570-900, BrazilDepartamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa 36570-900, BrazilDepartamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa 36570-900, BrazilDepartamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa 36570-900, BrazilDepartamento de Fitossanidade, Universidade Federal de Pelotas, Capão do Leão 96160-000, BrazilDepartamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa 36570-900, BrazilBegomoviruses can be found in association with alphasatellites, which are capable of autonomous replication but are dependent on the helper begomovirus for systemic infection, encapsidation and vector transmission. Previous studies suggest that the presence of NW alphasatellites (genus <i>Clecrusatellite</i>) is associated with more severe symptoms. To better understand this interaction, we investigated the effects of two alphasatellites on infectivity, symptom development, viral DNA accumulation and vector transmission of three begomoviruses in three hosts. In tomato and <i>Nicotiana benthamiana</i>, all combinations were infectious. In <i>Leonurus sibiricus</i>, only the ToYSV/ToYSA combination was infectious. The presence of EuYMA increased symptom severity of EuYMV and ToYSV in <i>N. benthamiana</i>, and the presence of ToYSA was associated with more severe symptoms of ToYSV in <i>N. benthamiana</i> and <i>L. sibiricus</i>. EuYMA increased the accumulation of ToYSV in <i>N. benthamiana</i> but reduced the accumulation of EuYMV in tomato and of ToSRV in <i>N. benthamiana</i>. The presence of ToYSA decreased the accumulation of ToYSV in <i>N. benthamiana</i> and <i>L. sibiricus</i>. ToYSA negatively affected transmission of ToSRV by <i>Bemisia tabaci</i> MEAM1. Together, our results indicate that NW alphasatellites can interact with different begomoviruses, increasing symptom severity and interfering in the transmission of the helper begomovirus. Understanding this interaction is important as it may affect the emergence of diseases caused by begomovirus–alphasatellite complexes in the field.https://www.mdpi.com/2076-0817/10/10/1244clecrusatellitegeminiviruswhiteflynatural hostexperimental hostfitness |
| spellingShingle | Angélica M. Nogueira Monique B. Nascimento Tarsiane M. C. Barbosa Ayane F. F. Quadros João Paulo A. Gomes Anelise F. Orílio Danielle R. Barros Francisco Murilo Zerbini The Association between New World Alphasatellites and Bipartite Begomoviruses: Effects on Infection and Vector Transmission Pathogens clecrusatellite geminivirus whitefly natural host experimental host fitness |
| title | The Association between New World Alphasatellites and Bipartite Begomoviruses: Effects on Infection and Vector Transmission |
| title_full | The Association between New World Alphasatellites and Bipartite Begomoviruses: Effects on Infection and Vector Transmission |
| title_fullStr | The Association between New World Alphasatellites and Bipartite Begomoviruses: Effects on Infection and Vector Transmission |
| title_full_unstemmed | The Association between New World Alphasatellites and Bipartite Begomoviruses: Effects on Infection and Vector Transmission |
| title_short | The Association between New World Alphasatellites and Bipartite Begomoviruses: Effects on Infection and Vector Transmission |
| title_sort | association between new world alphasatellites and bipartite begomoviruses effects on infection and vector transmission |
| topic | clecrusatellite geminivirus whitefly natural host experimental host fitness |
| url | https://www.mdpi.com/2076-0817/10/10/1244 |
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