Host Transcriptional Response of Sclerotinia sclerotiorum Induced by the Mycoparasite Coniothyrium minitans
Mycoparasite Coniothyrium minitans parasitizes specifically the mycelia or sclerotia of Sclerotinia sclerotiorum, a worldwidely spread plant fungal pathogen causing serious diseases on crops. The interaction of C. minitans with S. sclerotiorum remains reciprocal and complex and little is known, espe...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-02-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.00183/full |
| _version_ | 1818061603653812224 |
|---|---|
| author | Huizhang Zhao Huizhang Zhao Ting Zhou Ting Zhou Jiatao Xie Jiatao Xie Jiasen Cheng Jiasen Cheng Daohong Jiang Daohong Jiang Yanping Fu |
| author_facet | Huizhang Zhao Huizhang Zhao Ting Zhou Ting Zhou Jiatao Xie Jiatao Xie Jiasen Cheng Jiasen Cheng Daohong Jiang Daohong Jiang Yanping Fu |
| author_sort | Huizhang Zhao |
| collection | DOAJ |
| description | Mycoparasite Coniothyrium minitans parasitizes specifically the mycelia or sclerotia of Sclerotinia sclerotiorum, a worldwidely spread plant fungal pathogen causing serious diseases on crops. The interaction of C. minitans with S. sclerotiorum remains reciprocal and complex and little is known, especially on the side of the host (S. sclerotiorum). In this study, the early transcriptional response of S. sclerotiorum to the mycoparasitism by C. minitans was explored and the differentially expressed genes (DEGs) were analyzed. Based on GO ontology, KEGG pathway and fungal categories database, 887 up-regulated DEGs were enriched in the growth related function (i.e., rRNA processing, ribosome biogenesis, binding and transport), while the 546 down-regulated DEGs were enriched in the stress-related functions (i.e., oxidoreductase, response to stress and heat and the chorismate biosynthetic process). The expression of shikimate pathway and the biosynthesis of phenylalanine involving genes was significantly suppressed. Furthermore, 581 unenriched DEGs were explored in the parasitizing process and were mapped on the Pfam domains of redox enzymes, Alpha/Beta hydrolase, haloacid dehalogenase, and other universal conserved domain containing proteins. Thirty-two DEGs encoding candidate effectors, with 16 up-regulated and 16 down-regulated, were observed with diverse function. SS1G_11912 (encoding SsNEP2) was significantly up-regulated and may function in the parasitism. The involving of the shikimate pathway of phenylalanine biosynthesis and effector candidates were discussed. The results provide a basal understand on the interaction of S. sclerotiorum and C. minitans. |
| first_indexed | 2024-12-10T13:50:57Z |
| format | Article |
| id | doaj.art-5e3fb915306e4ce79ba695a1264e4d6b |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T13:50:57Z |
| publishDate | 2020-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5e3fb915306e4ce79ba695a1264e4d6b2022-12-22T01:46:10ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-02-011110.3389/fmicb.2020.00183512040Host Transcriptional Response of Sclerotinia sclerotiorum Induced by the Mycoparasite Coniothyrium minitansHuizhang Zhao0Huizhang Zhao1Ting Zhou2Ting Zhou3Jiatao Xie4Jiatao Xie5Jiasen Cheng6Jiasen Cheng7Daohong Jiang8Daohong Jiang9Yanping Fu10State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaHubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaHubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaHubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaHubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaHubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaHubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaMycoparasite Coniothyrium minitans parasitizes specifically the mycelia or sclerotia of Sclerotinia sclerotiorum, a worldwidely spread plant fungal pathogen causing serious diseases on crops. The interaction of C. minitans with S. sclerotiorum remains reciprocal and complex and little is known, especially on the side of the host (S. sclerotiorum). In this study, the early transcriptional response of S. sclerotiorum to the mycoparasitism by C. minitans was explored and the differentially expressed genes (DEGs) were analyzed. Based on GO ontology, KEGG pathway and fungal categories database, 887 up-regulated DEGs were enriched in the growth related function (i.e., rRNA processing, ribosome biogenesis, binding and transport), while the 546 down-regulated DEGs were enriched in the stress-related functions (i.e., oxidoreductase, response to stress and heat and the chorismate biosynthetic process). The expression of shikimate pathway and the biosynthesis of phenylalanine involving genes was significantly suppressed. Furthermore, 581 unenriched DEGs were explored in the parasitizing process and were mapped on the Pfam domains of redox enzymes, Alpha/Beta hydrolase, haloacid dehalogenase, and other universal conserved domain containing proteins. Thirty-two DEGs encoding candidate effectors, with 16 up-regulated and 16 down-regulated, were observed with diverse function. SS1G_11912 (encoding SsNEP2) was significantly up-regulated and may function in the parasitism. The involving of the shikimate pathway of phenylalanine biosynthesis and effector candidates were discussed. The results provide a basal understand on the interaction of S. sclerotiorum and C. minitans.https://www.frontiersin.org/article/10.3389/fmicb.2020.00183/fullSclerotinia sclerotiorumConiothyrium minitansshikimate pathwayeffectornecrosis- and ethylene-inducing peptide 1mycoparasitism |
| spellingShingle | Huizhang Zhao Huizhang Zhao Ting Zhou Ting Zhou Jiatao Xie Jiatao Xie Jiasen Cheng Jiasen Cheng Daohong Jiang Daohong Jiang Yanping Fu Host Transcriptional Response of Sclerotinia sclerotiorum Induced by the Mycoparasite Coniothyrium minitans Frontiers in Microbiology Sclerotinia sclerotiorum Coniothyrium minitans shikimate pathway effector necrosis- and ethylene-inducing peptide 1 mycoparasitism |
| title | Host Transcriptional Response of Sclerotinia sclerotiorum Induced by the Mycoparasite Coniothyrium minitans |
| title_full | Host Transcriptional Response of Sclerotinia sclerotiorum Induced by the Mycoparasite Coniothyrium minitans |
| title_fullStr | Host Transcriptional Response of Sclerotinia sclerotiorum Induced by the Mycoparasite Coniothyrium minitans |
| title_full_unstemmed | Host Transcriptional Response of Sclerotinia sclerotiorum Induced by the Mycoparasite Coniothyrium minitans |
| title_short | Host Transcriptional Response of Sclerotinia sclerotiorum Induced by the Mycoparasite Coniothyrium minitans |
| title_sort | host transcriptional response of sclerotinia sclerotiorum induced by the mycoparasite coniothyrium minitans |
| topic | Sclerotinia sclerotiorum Coniothyrium minitans shikimate pathway effector necrosis- and ethylene-inducing peptide 1 mycoparasitism |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.00183/full |
| work_keys_str_mv | AT huizhangzhao hosttranscriptionalresponseofsclerotiniasclerotioruminducedbythemycoparasiteconiothyriumminitans AT huizhangzhao hosttranscriptionalresponseofsclerotiniasclerotioruminducedbythemycoparasiteconiothyriumminitans AT tingzhou hosttranscriptionalresponseofsclerotiniasclerotioruminducedbythemycoparasiteconiothyriumminitans AT tingzhou hosttranscriptionalresponseofsclerotiniasclerotioruminducedbythemycoparasiteconiothyriumminitans AT jiataoxie hosttranscriptionalresponseofsclerotiniasclerotioruminducedbythemycoparasiteconiothyriumminitans AT jiataoxie hosttranscriptionalresponseofsclerotiniasclerotioruminducedbythemycoparasiteconiothyriumminitans AT jiasencheng hosttranscriptionalresponseofsclerotiniasclerotioruminducedbythemycoparasiteconiothyriumminitans AT jiasencheng hosttranscriptionalresponseofsclerotiniasclerotioruminducedbythemycoparasiteconiothyriumminitans AT daohongjiang hosttranscriptionalresponseofsclerotiniasclerotioruminducedbythemycoparasiteconiothyriumminitans AT daohongjiang hosttranscriptionalresponseofsclerotiniasclerotioruminducedbythemycoparasiteconiothyriumminitans AT yanpingfu hosttranscriptionalresponseofsclerotiniasclerotioruminducedbythemycoparasiteconiothyriumminitans |