Rubia cordifolia L. ameliorates DSS-induced ulcerative colitis in mice through dual inhibition of NLRP3 inflammasome and IL-6/JAK2/STAT3 pathways
The aerial part of Rubia cordifolia L. has been used as an herbal medicine for a long time with various pharmacological activities, including anti-inflammatory, anticancer, and antibacterial activities. The most notable usage of these was that this herbal medicine had good therapeutic effects on d...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-08-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022016024 |
| _version_ | 1811188145145774080 |
|---|---|
| author | Wei Qin Han Luo Liu Yang Di Hu Su-Ping Jiang Dai-Yin Peng Jiang-Miao Hu Shou-Jin Liu |
| author_facet | Wei Qin Han Luo Liu Yang Di Hu Su-Ping Jiang Dai-Yin Peng Jiang-Miao Hu Shou-Jin Liu |
| author_sort | Wei Qin |
| collection | DOAJ |
| description | The aerial part of Rubia cordifolia L. has been used as an herbal medicine for a long time with various pharmacological activities, including anti-inflammatory, anticancer, and antibacterial activities. The most notable usage of these was that this herbal medicine had good therapeutic effects on diarrhea caused by various factors. However, the mechanism for the ethanolic extract of R. cordifolia L. (RCEE) to treat Ulcerative colitis (UC) effectively is still unclear. In this study, DSS successfully induced UC mice and then intervene using different polar parts of RCEE. The results indicated that RCEE-treatment inhibited colonic combination NLRP3 inflammasome formation and IL-6/JAK2/STAT3 activation in vivo, significantly ameliorating the clinical symptoms, including alleviating colonic mucosal damage and infiltration of macrophages, suppressing the release of inflammatory cytokines, and reducing mortality. Taken together, this study suggests that dual inhibition of NLRP3 inflammasome and IL-6/JAK2/STAT3 pathways activation using RCEE may be a promising therapeutic strategy for preventing the progression of UC. |
| first_indexed | 2024-04-11T14:15:49Z |
| format | Article |
| id | doaj.art-5e4148f171264747bf08cb7db6952c75 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-11T14:15:49Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-5e4148f171264747bf08cb7db6952c752022-12-22T04:19:33ZengElsevierHeliyon2405-84402022-08-0188e10314Rubia cordifolia L. ameliorates DSS-induced ulcerative colitis in mice through dual inhibition of NLRP3 inflammasome and IL-6/JAK2/STAT3 pathwaysWei Qin0Han Luo1Liu Yang2Di Hu3Su-Ping Jiang4Dai-Yin Peng5Jiang-Miao Hu6Shou-Jin Liu7Faculty of Pharmaceutical, Anhui University of Chinese Medicine, Hefei 230012, ChinaFaculty of Pharmaceutical, Anhui University of Chinese Medicine, Hefei 230012, ChinaState Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaFaculty of Pharmaceutical, Yunnan University of Chinese Medicine, Kunming 530100, ChinaFaculty of Pharmaceutical, Anhui University of Chinese Medicine, Hefei 230012, ChinaFaculty of Pharmaceutical, Anhui University of Chinese Medicine, Hefei 230012, China; Institute of Traditional Chinese Medicine Resources Protection and Development, Anhui Academy of Chinese Medicine, Hefei 230012, ChinaState Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; Corresponding author.Faculty of Pharmaceutical, Anhui University of Chinese Medicine, Hefei 230012, China; Institute of Traditional Chinese Medicine Resources Protection and Development, Anhui Academy of Chinese Medicine, Hefei 230012, China; Corresponding author.The aerial part of Rubia cordifolia L. has been used as an herbal medicine for a long time with various pharmacological activities, including anti-inflammatory, anticancer, and antibacterial activities. The most notable usage of these was that this herbal medicine had good therapeutic effects on diarrhea caused by various factors. However, the mechanism for the ethanolic extract of R. cordifolia L. (RCEE) to treat Ulcerative colitis (UC) effectively is still unclear. In this study, DSS successfully induced UC mice and then intervene using different polar parts of RCEE. The results indicated that RCEE-treatment inhibited colonic combination NLRP3 inflammasome formation and IL-6/JAK2/STAT3 activation in vivo, significantly ameliorating the clinical symptoms, including alleviating colonic mucosal damage and infiltration of macrophages, suppressing the release of inflammatory cytokines, and reducing mortality. Taken together, this study suggests that dual inhibition of NLRP3 inflammasome and IL-6/JAK2/STAT3 pathways activation using RCEE may be a promising therapeutic strategy for preventing the progression of UC.http://www.sciencedirect.com/science/article/pii/S2405844022016024Rubia cordifolia L.Ulcerative colitisNLRP3 inflammasomeIL-6/JAK2/STAT3 pathways |
| spellingShingle | Wei Qin Han Luo Liu Yang Di Hu Su-Ping Jiang Dai-Yin Peng Jiang-Miao Hu Shou-Jin Liu Rubia cordifolia L. ameliorates DSS-induced ulcerative colitis in mice through dual inhibition of NLRP3 inflammasome and IL-6/JAK2/STAT3 pathways Heliyon Rubia cordifolia L. Ulcerative colitis NLRP3 inflammasome IL-6/JAK2/STAT3 pathways |
| title | Rubia cordifolia L. ameliorates DSS-induced ulcerative colitis in mice through dual inhibition of NLRP3 inflammasome and IL-6/JAK2/STAT3 pathways |
| title_full | Rubia cordifolia L. ameliorates DSS-induced ulcerative colitis in mice through dual inhibition of NLRP3 inflammasome and IL-6/JAK2/STAT3 pathways |
| title_fullStr | Rubia cordifolia L. ameliorates DSS-induced ulcerative colitis in mice through dual inhibition of NLRP3 inflammasome and IL-6/JAK2/STAT3 pathways |
| title_full_unstemmed | Rubia cordifolia L. ameliorates DSS-induced ulcerative colitis in mice through dual inhibition of NLRP3 inflammasome and IL-6/JAK2/STAT3 pathways |
| title_short | Rubia cordifolia L. ameliorates DSS-induced ulcerative colitis in mice through dual inhibition of NLRP3 inflammasome and IL-6/JAK2/STAT3 pathways |
| title_sort | rubia cordifolia l ameliorates dss induced ulcerative colitis in mice through dual inhibition of nlrp3 inflammasome and il 6 jak2 stat3 pathways |
| topic | Rubia cordifolia L. Ulcerative colitis NLRP3 inflammasome IL-6/JAK2/STAT3 pathways |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022016024 |
| work_keys_str_mv | AT weiqin rubiacordifolialamelioratesdssinducedulcerativecolitisinmicethroughdualinhibitionofnlrp3inflammasomeandil6jak2stat3pathways AT hanluo rubiacordifolialamelioratesdssinducedulcerativecolitisinmicethroughdualinhibitionofnlrp3inflammasomeandil6jak2stat3pathways AT liuyang rubiacordifolialamelioratesdssinducedulcerativecolitisinmicethroughdualinhibitionofnlrp3inflammasomeandil6jak2stat3pathways AT dihu rubiacordifolialamelioratesdssinducedulcerativecolitisinmicethroughdualinhibitionofnlrp3inflammasomeandil6jak2stat3pathways AT supingjiang rubiacordifolialamelioratesdssinducedulcerativecolitisinmicethroughdualinhibitionofnlrp3inflammasomeandil6jak2stat3pathways AT daiyinpeng rubiacordifolialamelioratesdssinducedulcerativecolitisinmicethroughdualinhibitionofnlrp3inflammasomeandil6jak2stat3pathways AT jiangmiaohu rubiacordifolialamelioratesdssinducedulcerativecolitisinmicethroughdualinhibitionofnlrp3inflammasomeandil6jak2stat3pathways AT shoujinliu rubiacordifolialamelioratesdssinducedulcerativecolitisinmicethroughdualinhibitionofnlrp3inflammasomeandil6jak2stat3pathways |