Interaction of <it>Hm</it>C1q with leech microglial cells: involvement of C1qBP-related molecule in the induction of cell chemotaxis
<p>Abstract</p> <p>Background</p> <p>In invertebrates, the medicinal leech is considered to be an interesting and appropriate model to study neuroimmune mechanisms. Indeed, this non-vertebrate animal can restore normal function of its central nervous system (CNS) after...
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BMC
2012-02-01
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Series: | Journal of Neuroinflammation |
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Online Access: | http://www.jneuroinflammation.com/content/9/1/37 |
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author | Tahtouh Muriel Garçon-Bocquet Annelise Croq Françoise Vizioli Jacopo Sautière Pierre-Eric Van Camp Christelle Salzet Michel Nagnan-le Meillour Patricia Pestel Joël Lefebvre Christophe |
author_facet | Tahtouh Muriel Garçon-Bocquet Annelise Croq Françoise Vizioli Jacopo Sautière Pierre-Eric Van Camp Christelle Salzet Michel Nagnan-le Meillour Patricia Pestel Joël Lefebvre Christophe |
author_sort | Tahtouh Muriel |
collection | DOAJ |
description | <p>Abstract</p> <p>Background</p> <p>In invertebrates, the medicinal leech is considered to be an interesting and appropriate model to study neuroimmune mechanisms. Indeed, this non-vertebrate animal can restore normal function of its central nervous system (CNS) after injury. Microglia accumulation at the damage site has been shown to be required for axon sprouting and for efficient regeneration. We characterized <it>Hm</it>C1q as a novel chemotactic factor for leech microglial cell recruitment. In mammals, a C1q-binding protein (C1qBP alias gC1qR), which interacts with the globular head of C1q, has been reported to participate in C1q-mediated chemotaxis of blood immune cells. In this study, we evaluated the chemotactic activities of a recombinant form of <it>Hm</it>C1q and its interaction with a newly characterized leech C1qBP that acts as its potential ligand.</p> <p>Methods</p> <p>Recombinant <it>Hm</it>C1q (r<it>Hm</it>C1q) was produced in the yeast <it>Pichia pastoris</it>. Chemotaxis assays were performed to investigate r<it>Hm</it>C1q-dependent microglia migration. The involvement of a C1qBP-related molecule in this chemotaxis mechanism was assessed by flow cytometry and with affinity purification experiments. The cellular localization of C1qBP mRNA and protein in leech was investigated using immunohistochemistry and <it>in situ </it>hybridization techniques.</p> <p>Results</p> <p>r<it>Hm</it>C1q-stimulated microglia migrate in a dose-dependent manner. This r<it>Hm</it>C1q-induced chemotaxis was reduced when cells were preincubated with either anti-<it>Hm</it>C1q or anti-human C1qBP antibodies. A C1qBP-related molecule was characterized in leech microglia.</p> <p>Conclusions</p> <p>A previous study showed that recruitment of microglia is observed after <it>Hm</it>C1q release at the cut end of axons. Here, we demonstrate that r<it>Hm</it>C1q-dependent chemotaxis might be driven via a <it>Hm</it>C1q-binding protein located on the microglial cell surface. Taken together, these results highlight the importance of the interaction between C1q and C1qBP in microglial activation leading to nerve repair in the medicinal leech.</p> |
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issn | 1742-2094 |
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publishDate | 2012-02-01 |
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series | Journal of Neuroinflammation |
spelling | doaj.art-5e41a0ac75894584b44c2684c1b3a5e52022-12-21T19:07:37ZengBMCJournal of Neuroinflammation1742-20942012-02-01913710.1186/1742-2094-9-37Interaction of <it>Hm</it>C1q with leech microglial cells: involvement of C1qBP-related molecule in the induction of cell chemotaxisTahtouh MurielGarçon-Bocquet AnneliseCroq FrançoiseVizioli JacopoSautière Pierre-EricVan Camp ChristelleSalzet MichelNagnan-le Meillour PatriciaPestel JoëlLefebvre Christophe<p>Abstract</p> <p>Background</p> <p>In invertebrates, the medicinal leech is considered to be an interesting and appropriate model to study neuroimmune mechanisms. Indeed, this non-vertebrate animal can restore normal function of its central nervous system (CNS) after injury. Microglia accumulation at the damage site has been shown to be required for axon sprouting and for efficient regeneration. We characterized <it>Hm</it>C1q as a novel chemotactic factor for leech microglial cell recruitment. In mammals, a C1q-binding protein (C1qBP alias gC1qR), which interacts with the globular head of C1q, has been reported to participate in C1q-mediated chemotaxis of blood immune cells. In this study, we evaluated the chemotactic activities of a recombinant form of <it>Hm</it>C1q and its interaction with a newly characterized leech C1qBP that acts as its potential ligand.</p> <p>Methods</p> <p>Recombinant <it>Hm</it>C1q (r<it>Hm</it>C1q) was produced in the yeast <it>Pichia pastoris</it>. Chemotaxis assays were performed to investigate r<it>Hm</it>C1q-dependent microglia migration. The involvement of a C1qBP-related molecule in this chemotaxis mechanism was assessed by flow cytometry and with affinity purification experiments. The cellular localization of C1qBP mRNA and protein in leech was investigated using immunohistochemistry and <it>in situ </it>hybridization techniques.</p> <p>Results</p> <p>r<it>Hm</it>C1q-stimulated microglia migrate in a dose-dependent manner. This r<it>Hm</it>C1q-induced chemotaxis was reduced when cells were preincubated with either anti-<it>Hm</it>C1q or anti-human C1qBP antibodies. A C1qBP-related molecule was characterized in leech microglia.</p> <p>Conclusions</p> <p>A previous study showed that recruitment of microglia is observed after <it>Hm</it>C1q release at the cut end of axons. Here, we demonstrate that r<it>Hm</it>C1q-dependent chemotaxis might be driven via a <it>Hm</it>C1q-binding protein located on the microglial cell surface. Taken together, these results highlight the importance of the interaction between C1q and C1qBP in microglial activation leading to nerve repair in the medicinal leech.</p>http://www.jneuroinflammation.com/content/9/1/37C1qC1qBP (alias gC1qR)ChemotaxisMedicinal leechMicrogliaNerve repairNeuroinflammation |
spellingShingle | Tahtouh Muriel Garçon-Bocquet Annelise Croq Françoise Vizioli Jacopo Sautière Pierre-Eric Van Camp Christelle Salzet Michel Nagnan-le Meillour Patricia Pestel Joël Lefebvre Christophe Interaction of <it>Hm</it>C1q with leech microglial cells: involvement of C1qBP-related molecule in the induction of cell chemotaxis Journal of Neuroinflammation C1q C1qBP (alias gC1qR) Chemotaxis Medicinal leech Microglia Nerve repair Neuroinflammation |
title | Interaction of <it>Hm</it>C1q with leech microglial cells: involvement of C1qBP-related molecule in the induction of cell chemotaxis |
title_full | Interaction of <it>Hm</it>C1q with leech microglial cells: involvement of C1qBP-related molecule in the induction of cell chemotaxis |
title_fullStr | Interaction of <it>Hm</it>C1q with leech microglial cells: involvement of C1qBP-related molecule in the induction of cell chemotaxis |
title_full_unstemmed | Interaction of <it>Hm</it>C1q with leech microglial cells: involvement of C1qBP-related molecule in the induction of cell chemotaxis |
title_short | Interaction of <it>Hm</it>C1q with leech microglial cells: involvement of C1qBP-related molecule in the induction of cell chemotaxis |
title_sort | interaction of it hm it c1q with leech microglial cells involvement of c1qbp related molecule in the induction of cell chemotaxis |
topic | C1q C1qBP (alias gC1qR) Chemotaxis Medicinal leech Microglia Nerve repair Neuroinflammation |
url | http://www.jneuroinflammation.com/content/9/1/37 |
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